Invitrogen

SIRT7 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

SIRT7 Antibody in Western Blot (WB) — Western Blot analysis of SIRT7 in extracts of various cell lines using SIRT7 Polyclonal Antibody (Product # PA5-87543) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Details

PA5-87543

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 201-400 of human SIRT7 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.43 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.05% ProClin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2804240

Product Specific Information

Immunogen sequence: NREYVRVFDV TERTALHRHQ TGRTCHKCGT QLRDTIVHFG ERGTLGQPLN WEAATEAASR ADTILCLGSS LKVLKKYPRL WCMTKPPSRR PKLYIVNLQW TPKDDWAALK LHGKCDDVMR LLMAELGLEI PAYSRWQDPI FSLATPLRAG EEGSHSRKSL CRSREEAPPG DRGAPLSSAP ILGGWFGRGC TKRTKRKKVT; Positive Samples: HepG2, SW480; Cellular Location: Cytoplasm, Nucleus, nucleolus

Target Information

SIRT7 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT7 is included in class IV. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. SIRT7 is included in class IV of the sirtuin family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sirtuin 7 Regulates Nitric Oxide Production and Apoptosis to Promote Mycobacterial Clearance in Macrophages.

Frontiers in immunology

Zhang S,Liu Y,Zhou X,Ou M,Xiao G,Li F,Wang Z,Wang Z,Liu L,Zhang G

PA5-87543 was used in Western Blot to show that SIRT7 has potential to be exploited as a novel effective target for HDT of TB.

Wed Feb 16 00:00:00 EST 2022

Sirtuin 7 Regulates Nitric Oxide Production and Apoptosis to Promote Mycobacterial Clearance in Macrophages.

Frontiers in immunology

Zhang S,Liu Y,Zhou X,Ou M,Xiao G,Li F,Wang Z,Wang Z,Liu L,Zhang G

PA5-87543 was used in Western Blot to show that SIRT7 has potential to be exploited as a novel effective target for HDT of TB.

Wed Feb 16 00:00:00 EST 2022

Bioinformatics

Protein Aliases: MGC126840; MGC126842; NAD-dependent deacetylase sirtuin-7; NAD-dependent protein deacetylase sirtuin-7; NAD-dependent protein deacylase sirtuin-7; Regulatory protein SIR2 homolog 7; silent mating type information regulation 2, S.cerevisiae, homolog 7; SIR2-like protein 7; sir2-related protein type 7; sirtuin type 7

Gene Aliases: SIR2L7; SIRT7

UniProt ID: (Human) Q9NRC8, (Mouse) Q8BKJ9, (Rat) B2RZ55

Entrez Gene ID: (Human) 51547, (Mouse) 209011, (Rat) 303745

Function(s)

negative regulation of transcription from RNA polymerase II promoter positive regulation of transcription involved in exit from mitosis rRNA transcription histone H3 deacetylation histone H4 deacetylation transcription, DNA-templated regulation of transcription, DNA-templated chromatin modification biological_process

Process(es)

SIRT7 Polyclonal Antibody

Product Details

PA5-87543

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!