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SIX1 Polyclonal Antibody

View all (11) SIX1 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (15)

SIX1 Antibody in Immunocytochemistry (ICC/IF)

SIX1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

SIX1 Antibody (10709-1-AP) in ICC/IF

IF result of anti-SIX1 (1:50) in p53-/- mammary epithelial tumor cells. Blue: hoechst nuclear stain, Red: SIX1 stain the mesenchymal cell which have undergone EMT.

SIX1 Antibody in Immunocytochemistry (ICC/IF)

SIX1 Antibody in Western Blot (WB)

SIX1 Antibody in Western Blot (WB)

SIX1 Antibody in Western Blot (WB)

SIX1 Antibody in Western Blot (WB)

SIX1 Antibody in Western Blot (WB)

SIX1 Antibody in Western Blot (WB)

SIX1 Antibody in Western Blot (WB)

SIX1 Antibody in Western Blot (WB)

SIX1 Antibody in Western Blot (WB)

SIX1 Antibody in Western Blot (WB)

SIX1 Antibody in Western Blot (WB)

SIX1 Antibody in Western Blot (WB)

SIX1 Antibody in Western Blot (WB)

SIX1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

SIX1 Antibody in Immunocytochemistry (ICC/IF) — IF result of anti-SIX1 (1:50) in p53-/- mammary epithelial tumor cells. Blue: hoechst nuclear stain, Red: SIX1 stain the mesenchymal cell which have undergone EMT.

SIX1 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — PC-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — PC-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — A2780 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Immunoprecipitation (IP) — IP result of anti-SIX1 (IP:10709-1-AP, 3ug; Detection:10709-1-AP 1:1000) with HEK-293 cells lysate 2560ug.

Product Details

10709-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

Assay-dependent

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

1:20-1:200

View 7 publications 7 publications

Immunoprecipitation (IP)

0.5-4.0 µg

-

Not applicable (N/A)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SIX1 Fusion Protein Ag1044 (1-284 aa encoded by BC008874)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSMLPSFGF TQEQVACVCE VLQQGGNLER LGRFLWSLPA CDHLHKNESV LKAKAVVAFH RGNFRELYKI LESHQFSPHN HPKLQQLWLK AHYVEAEKLC GRPLGAVGKY RVRRKFPLPR TIWDGEETSY CFKEKSRGVL REWYAHNPYP SPREKRELAE ATGLTTTQVS NWFKNRRQRD RAAEAKEREN TENNNSSSNK QNQLSPLEGG KPLMSSSEEE FSPPQSPDQN SVLLLQGNMG HARSSNYSLP GLTASQPSHG LQTHQHQLQD SLLGPLTSSL VDLGS (1-284 aa encoded by BC008874)

Target Information

The protein encoded by this gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identity, lineage and fates of a temporally distinct progenitor population in the embryonic olfactory epithelium.

Developmental biology

Paronett EM,Bryan CA,Maynard TM,LaMantia AS

Wed Mar 01 00:00:00 UTC 2023

DNAAF5 promotes hepatocellular carcinoma malignant progression by recruiting USP39 to improve PFKL protein stability.

Frontiers in oncology

Liu Y,Wu Q,Sun T,Huang J,Han G,Han H

Tue Oct 25 00:00:00 UTC 2022

Tanshinone IIA inhibits cell growth by suppressing SIX1-induced aerobic glycolysis in non-small cell lung cancer cells.

Oncology letters

Qi H,Chen Z,Qin Y,Wang X,Zhang Z,Li Y

Wed Jun 01 00:00:00 UTC 2022

Ginsenoside Rh4 Suppresses Metastasis of Gastric Cancer via SIX1-Dependent TGF-β/Smad2/3 Signaling Pathway.

Nutrients

Jiang H,Ma P,Duan Z,Liu Y,Shen S,Mi Y,Fan D

Sat Apr 09 00:00:00 UTC 2022

Ginsenoside Rh4 Suppresses Metastasis of Gastric Cancer via SIX1-Dependent TGF-β/Smad2/3 Signaling Pathway.

Nutrients

Jiang H,Ma P,Duan Z,Liu Y,Shen S,Mi Y,Fan D

Sat Apr 09 00:00:00 UTC 2022

Ginsenoside Rh4 Suppresses Metastasis of Gastric Cancer via SIX1-Dependent TGF-β/Smad2/3 Signaling Pathway.

Nutrients

Jiang H,Ma P,Duan Z,Liu Y,Shen S,Mi Y,Fan D

Sat Apr 09 00:00:00 UTC 2022

Ginsenoside Rh4 Suppresses Metastasis of Gastric Cancer via SIX1-Dependent TGF-β/Smad2/3 Signaling Pathway.

Nutrients

Jiang H,Ma P,Duan Z,Liu Y,Shen S,Mi Y,Fan D

Sat Apr 09 00:00:00 UTC 2022

Ginsenoside Rh4 Suppresses Metastasis of Gastric Cancer via SIX1-Dependent TGF-β/Smad2/3 Signaling Pathway.

Nutrients

Jiang H,Ma P,Duan Z,Liu Y,Shen S,Mi Y,Fan D

Sat Apr 09 00:00:00 UTC 2022

Ginsenoside Rh4 Suppresses Metastasis of Gastric Cancer via SIX1-Dependent TGF-β/Smad2/3 Signaling Pathway.

Nutrients

Jiang H,Ma P,Duan Z,Liu Y,Shen S,Mi Y,Fan D

Sat Apr 09 00:00:00 UTC 2022

Selective disruption of trigeminal sensory neurogenesis and differentiation in a mouse model of 22q11.2 deletion syndrome.

Disease models & mechanisms

Karpinski BA,Maynard TM,Bryan CA,Yitsege G,Horvath A,Lee NH,Moody SA,LaMantia AS

Tue Feb 01 00:00:00 UTC 2022

Nuclear TEAD4 with SIX1 Overexpression is an Independent Prognostic Marker in the Stage I-III Colorectal Cancer.

Cancer management and research

Yu T,Song J,Zhou H,Wu T,Liang Z,Du P,Liu CY,Wang G,Cui L,Liu Y

Wed Apr 20 00:00:00 UTC 2022

Nuclear TEAD4 with SIX1 Overexpression is an Independent Prognostic Marker in the Stage I-III Colorectal Cancer.

Cancer management and research

Yu T,Song J,Zhou H,Wu T,Liang Z,Du P,Liu CY,Wang G,Cui L,Liu Y

Wed Apr 20 00:00:00 UTC 2022

miR‑106b‑5p targeting SIX1 inhibits TGF‑β1‑induced pulmonary fibrosis and epithelial‑mesenchymal transition in asthma through regulation of E2F1.

International journal of molecular medicine

Liu S,Chen X,Zhang S,Wang X,Du X,Chen J,Zhou G

Mon Mar 01 00:00:00 UTC 2021

miR‑106b‑5p targeting SIX1 inhibits TGF‑β1‑induced pulmonary fibrosis and epithelial‑mesenchymal transition in asthma through regulation of E2F1.

International journal of molecular medicine

Liu S,Chen X,Zhang S,Wang X,Du X,Chen J,Zhou G

Mon Mar 01 00:00:00 UTC 2021

miR‑106b‑5p targeting SIX1 inhibits TGF‑β1‑induced pulmonary fibrosis and epithelial‑mesenchymal transition in asthma through regulation of E2F1.

International journal of molecular medicine

Liu S,Chen X,Zhang S,Wang X,Du X,Chen J,Zhou G

Mon Mar 01 00:00:00 UTC 2021

miR‑106b‑5p targeting SIX1 inhibits TGF‑β1‑induced pulmonary fibrosis and epithelial‑mesenchymal transition in asthma through regulation of E2F1.

International journal of molecular medicine

Liu S,Chen X,Zhang S,Wang X,Du X,Chen J,Zhou G

Mon Mar 01 00:00:00 UTC 2021

SIX1 Activation Is Involved in Cell Proliferation, Migration, and Anti-inflammation of Acute Ischemia/Reperfusion Injury in Mice.

Frontiers in molecular biosciences

Jin Y,Zhang M,Li M,Zhang H,Zhao L,Qian C,Li S,Zhang H,Gao M,Pan B,Li R,Wan X,Cao C

Tue Sep 14 00:00:00 UTC 2021

SIX1 Activation Is Involved in Cell Proliferation, Migration, and Anti-inflammation of Acute Ischemia/Reperfusion Injury in Mice.

Frontiers in molecular biosciences

Jin Y,Zhang M,Li M,Zhang H,Zhao L,Qian C,Li S,Zhang H,Gao M,Pan B,Li R,Wan X,Cao C

Tue Sep 14 00:00:00 UTC 2021

Muscle progenitor specification and myogenic differentiation are associated with changes in chromatin topology.

Nature communications

Zhang N,Mendieta-Esteban J,Magli A,Lilja KC,Perlingeiro RCR,Marti-Renom MA,Tsirigos A,Dynlacht BD

Fri Dec 04 00:00:00 UTC 2020

Transcriptional dysregulation in developing trigeminal sensory neurons in the LgDel mouse model of DiGeorge 22q11.2 deletion syndrome.

Human molecular genetics

Maynard TM,Horvath A,Bernot JP,Karpinski BA,Tavares ALP,Shah A,Zheng Q,Spurr L,Olender J,Moody SA,Fraser CM,LaMantia AS,Lee NH

Wed Apr 15 00:00:00 UTC 2020

O-GlcNAcylation of SIX1 enhances its stability and promotes Hepatocellular Carcinoma Proliferation.

Theranostics

Chu Y,Jiang M,Wu N,Xu B,Li W,Liu H,Su S,Shi Y,Liu H,Gao X,Fu X,Chen D,Li X,Wang W,Liang J,Nie Y,Fan D

Tue May 25 00:00:00 UTC 2021

miR-489-3p/SIX1 Axis Regulates Melanoma Proliferation and Glycolytic Potential.

Molecular therapy oncolytics

Yang X,Zhu X,Yan Z,Li C,Zhao H,Ma L,Zhang D,Liu J,Liu Z,Du N,Ye Q,Xu X

Fri Mar 27 00:00:00 UTC 2020

Aberrant early growth of individual trigeminal sensory and motor axons in a series of mouse genetic models of 22q11.2 deletion syndrome.

Human molecular genetics

Motahari Z,Maynard TM,Popratiloff A,Moody SA,LaMantia AS

Wed Nov 04 00:00:00 UTC 2020

Dynamic enhancers control skeletal muscle identity and reprogramming.

PLoS biology

Ramachandran K,Senagolage MD,Sommars MA,Futtner CR,Omura Y,Allred AL,Barish GD

Tue Oct 01 00:00:00 UTC 2019

Six1 is negatively correlated with poor prognosis and reduces 5-fluorouracil sensitivity via attenuating the stemness of hepatocellular carcinoma cells.

European journal of pharmacology

Chen K,Wei H,Pan J,Chen Z,Pan D,Gao T,Huang J,Huang M,Ou M,Zhong W

Tue Oct 15 00:00:00 UTC 2019

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Cancer cell

Li L,Liang Y,Kang L,Liu Y,Gao S,Chen S,Li Y,You W,Dong Q,Hong T,Yan Z,Jin S,Wang T,Zhao W,Mai H,Huang J,Han X,Ji Q,Song Q,Yang C,Zhao S,Xu X,Ye Q

Mon Mar 12 00:00:00 UTC 2018

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Cancer cell

Li L,Liang Y,Kang L,Liu Y,Gao S,Chen S,Li Y,You W,Dong Q,Hong T,Yan Z,Jin S,Wang T,Zhao W,Mai H,Huang J,Han X,Ji Q,Song Q,Yang C,Zhao S,Xu X,Ye Q

Mon Mar 12 00:00:00 UTC 2018

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Cancer cell

Li L,Liang Y,Kang L,Liu Y,Gao S,Chen S,Li Y,You W,Dong Q,Hong T,Yan Z,Jin S,Wang T,Zhao W,Mai H,Huang J,Han X,Ji Q,Song Q,Yang C,Zhao S,Xu X,Ye Q

Mon Mar 12 00:00:00 UTC 2018

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Cancer cell

Li L,Liang Y,Kang L,Liu Y,Gao S,Chen S,Li Y,You W,Dong Q,Hong T,Yan Z,Jin S,Wang T,Zhao W,Mai H,Huang J,Han X,Ji Q,Song Q,Yang C,Zhao S,Xu X,Ye Q

Mon Mar 12 00:00:00 UTC 2018

miR-204-5p suppresses hepatocellular cancer proliferation by regulating homeoprotein SIX1 expression.

FEBS open bio

Chu Y,Jiang M,Du F,Chen D,Ye T,Xu B,Li X,Wang W,Qiu Z,Liu H,Nie Y,Liang J,Fan D

Thu Feb 01 00:00:00 UTC 2018

miR-204-5p suppresses hepatocellular cancer proliferation by regulating homeoprotein SIX1 expression.

FEBS open bio

Chu Y,Jiang M,Du F,Chen D,Ye T,Xu B,Li X,Wang W,Qiu Z,Liu H,Nie Y,Liang J,Fan D

Thu Feb 01 00:00:00 UTC 2018

miR-204-5p suppresses hepatocellular cancer proliferation by regulating homeoprotein SIX1 expression.

FEBS open bio

Chu Y,Jiang M,Du F,Chen D,Ye T,Xu B,Li X,Wang W,Qiu Z,Liu H,Nie Y,Liang J,Fan D

Thu Feb 01 00:00:00 UTC 2018

miR-204-5p suppresses hepatocellular cancer proliferation by regulating homeoprotein SIX1 expression.

FEBS open bio

Chu Y,Jiang M,Du F,Chen D,Ye T,Xu B,Li X,Wang W,Qiu Z,Liu H,Nie Y,Liang J,Fan D

Thu Feb 01 00:00:00 UTC 2018

EYA4 Promotes Cell Proliferation Through Downregulation of p27Kip1 in Glioma.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Li Z,Qiu R,Qiu X,Tian T

Tue Oct 16 00:00:00 UTC 2018

EYA4 Promotes Cell Proliferation Through Downregulation of p27Kip1 in Glioma.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Li Z,Qiu R,Qiu X,Tian T

Tue Oct 16 00:00:00 UTC 2018

The homeoprotein SIX1 controls cellular senescence through the regulation of p16INK4A and differentiation-related genes.

Oncogene

Adrados I,Larrasa-Alonso J,Galarreta A,López-Antona I,Menéndez C,Abad M,Gil J,Moreno-Bueno G,Palmero I

Thu Jul 07 00:00:00 UTC 2016

The homeoprotein SIX1 controls cellular senescence through the regulation of p16INK4A and differentiation-related genes.

Oncogene

Adrados I,Larrasa-Alonso J,Galarreta A,López-Antona I,Menéndez C,Abad M,Gil J,Moreno-Bueno G,Palmero I

Thu Jul 07 00:00:00 UTC 2016

A cellular and molecular mosaic establishes growth and differentiation states for cranial sensory neurons.

Developmental biology

Karpinski BA,Bryan CA,Paronett EM,Baker JL,Fernandez A,Horvath A,Maynard TM,Moody SA,LaMantia AS

Fri Jul 15 00:00:00 UTC 2016

A cellular and molecular mosaic establishes growth and differentiation states for cranial sensory neurons.

Developmental biology

Karpinski BA,Bryan CA,Paronett EM,Baker JL,Fernandez A,Horvath A,Maynard TM,Moody SA,LaMantia AS

Fri Jul 15 00:00:00 UTC 2016

Sine oculis homeobox homolog 1 promotes α5β1-mediated invasive migration and metastasis of cervical cancer cells.

Biochemical and biophysical research communications

Liu D,Zhang XX,Wan DY,Xi BX,Ma D,Wang H,Gao QL

Fri Apr 04 00:00:00 UTC 2014

Sine oculis homeobox homolog 1 promotes α5β1-mediated invasive migration and metastasis of cervical cancer cells.

Biochemical and biophysical research communications

Liu D,Zhang XX,Wan DY,Xi BX,Ma D,Wang H,Gao QL

Fri Apr 04 00:00:00 UTC 2014

Expression of Six1 and Six4 in mouse taste buds.

Journal of molecular histology

Suzuki Y,Ikeda K,Kawakami K

Fri Oct 01 00:00:00 UTC 2010

Expression of Six1 and Six4 in mouse taste buds.

Journal of molecular histology

Suzuki Y,Ikeda K,Kawakami K

Fri Oct 01 00:00:00 UTC 2010

Expression of Six1 and Six4 in mouse taste buds.

Journal of molecular histology

Suzuki Y,Ikeda K,Kawakami K

Fri Oct 01 00:00:00 UTC 2010

Expression of Six1 and Six4 in mouse taste buds.

Journal of molecular histology

Suzuki Y,Ikeda K,Kawakami K

Fri Oct 01 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: Homeobox protein SIX1; Sine oculis homeobox homolog 1; sine oculis-related homeobox 1 homolog

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Gene Aliases: BB138287; BOS3; DFNA23; SIX1; TIP39

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UniProt ID: (Human) Q15475, (Mouse) Q62231

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Entrez Gene ID: (Human) 6495, (Mouse) 20471

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RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding transcription regulatory region DNA binding homeodomain transcription factor

negative regulation of transcription from RNA polymerase II promoter ureteric bud development branching involved in ureteric bud morphogenesis organ induction kidney development outflow tract morphogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter apoptotic process pattern specification process skeletal muscle tissue development sensory perception of sound cell proliferation regulation of synaptic growth at neuromuscular junction regulation of skeletal muscle satellite cell proliferation facial nerve morphogenesis epithelial cell differentiation thyroid gland development olfactory placode formation regulation of protein localization protein localization to nucleus aorta morphogenesis inner ear morphogenesis middle ear morphogenesis negative regulation of neuron apoptotic process regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter thymus development neuron fate specification generation of neurons embryonic cranial skeleton morphogenesis embryonic skeletal system morphogenesis inner ear development regulation of epithelial cell proliferation myoblast migration pharyngeal system development myotome development fungiform papilla morphogenesis trigeminal ganglion development otic vesicle development metanephric mesenchyme development regulation of branch elongation involved in ureteric bud branching positive regulation of ureteric bud formation mesonephric tubule formation ureter smooth muscle cell differentiation positive regulation of secondary heart field cardioblast proliferation cochlea morphogenesis positive regulation of branching involved in ureteric bud morphogenesis negative regulation of branching involved in ureteric bud morphogenesis positive regulation of mesenchymal cell proliferation involved in ureter development regulation of skeletal muscle cell differentiation transcription, DNA-templated multicellular organism development regulation of gene expression neurogenesis negative regulation of apoptotic process tongue development skeletal system morphogenesis anatomical structure development renal system development

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