Proteintech

SIX1 Polyclonal Antibody

View all (10) SIX1 antibodies

Questions & Answers

Tech Support

Questions & Answers

Tech Support

Product Image Gallery

SIX1 Antibody in Immunocytochemistry (ICC/IF) — IF result of anti-SIX1 (1:50) in p53-/- mammary epithelial tumor cells. Blue: hoechst nuclear stain, Red: SIX1 stain the mesenchymal cell which have undergone EMT.

SIX1 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — PC-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — PC-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — A2780 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Western Blot (WB) — L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SIX1 Antibody in Immunoprecipitation (IP) — IP result of anti-SIX1 (IP:10709-1-AP, 3ug; Detection:10709-1-AP 1:1000) with HEK-293 cells lysate 2560ug.

Product Details

10709-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 5 publications 5 publications
Immunohistochemistry (IHC)	Assay-dependent	View 3 publications 3 publications
Immunocytochemistry (ICC/IF)	1:20-1:200	View 2 publications 2 publications
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SIX1 Fusion Protein Ag1044 (1-284 aa encoded by BC008874) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MSMLPSFGF TQEQVACVCE VLQQGGNLER LGRFLWSLPA CDHLHKNESV LKAKAVVAFH RGNFRELYKI LESHQFSPHN HPKLQQLWLK AHYVEAEKLC GRPLGAVGKY RVRRKFPLPR TIWDGEETSY CFKEKSRGVL REWYAHNPYP SPREKRELAE ATGLTTTQVS NWFKNRRQRD RAAEAKEREN TENNNSSSNK QNQLSPLEGG KPLMSSSEEE FSPPQSPDQN SVLLLQGNMG HARSSNYSLP GLTASQPSHG LQTHQHQLQD SLLGPLTSSL VDLGS (1-284 aa encoded by BC008874)

Target Information

The protein encoded by this gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

EYA4 Promotes Cell Proliferation Through Downregulation of p27Kip1 in Glioma.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Li Z,Qiu R,Qiu X,Tian T

Tue Oct 16 00:00:00 EDT 2018

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Cancer cell

Li L,Liang Y,Kang L,Liu Y,Gao S,Chen S,Li Y,You W,Dong Q,Hong T,Yan Z,Jin S,Wang T,Zhao W,Mai H,Huang J,Han X,Ji Q,Song Q,Yang C,Zhao S,Xu X,Ye Q

Mon Mar 12 00:00:00 EDT 2018

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Cancer cell

Li L,Liang Y,Kang L,Liu Y,Gao S,Chen S,Li Y,You W,Dong Q,Hong T,Yan Z,Jin S,Wang T,Zhao W,Mai H,Huang J,Han X,Ji Q,Song Q,Yang C,Zhao S,Xu X,Ye Q

Mon Mar 12 00:00:00 EDT 2018

miR-204-5p suppresses hepatocellular cancer proliferation by regulating homeoprotein SIX1 expression.

FEBS open bio

Chu Y,Jiang M,Du F,Chen D,Ye T,Xu B,Li X,Wang W,Qiu Z,Liu H,Nie Y,Liang J,Fan D

Thu Feb 01 00:00:00 EST 2018

miR-204-5p suppresses hepatocellular cancer proliferation by regulating homeoprotein SIX1 expression.

FEBS open bio

Chu Y,Jiang M,Du F,Chen D,Ye T,Xu B,Li X,Wang W,Qiu Z,Liu H,Nie Y,Liang J,Fan D

Thu Feb 01 00:00:00 EST 2018

A cellular and molecular mosaic establishes growth and differentiation states for cranial sensory neurons.

Developmental biology

Karpinski BA,Bryan CA,Paronett EM,Baker JL,Fernandez A,Horvath A,Maynard TM,Moody SA,LaMantia AS

Fri Jul 15 00:00:00 EDT 2016

The homeoprotein SIX1 controls cellular senescence through the regulation of p16INK4A and differentiation-related genes.

Oncogene

Adrados I,Larrasa-Alonso J,Galarreta A,López-Antona I,Menéndez C,Abad M,Gil J,Moreno-Bueno G,Palmero I

Thu Jul 07 00:00:00 EDT 2016

Sine oculis homeobox homolog 1 promotes α5β1-mediated invasive migration and metastasis of cervical cancer cells.

Biochemical and biophysical research communications

Liu D,Zhang XX,Wan DY,Xi BX,Ma D,Wang H,Gao QL

Fri Apr 04 00:00:00 EDT 2014

Expression of Six1 and Six4 in mouse taste buds.

Journal of molecular histology

Suzuki Y,Ikeda K,Kawakami K

Fri Oct 01 00:00:00 EDT 2010

Expression of Six1 and Six4 in mouse taste buds.

Journal of molecular histology

Suzuki Y,Ikeda K,Kawakami K

Fri Oct 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Homeobox protein SIX1; Sine oculis homeobox homolog 1; sine oculis-related homeobox 1 homolog

Gene Aliases: BB138287; BOS3; DFNA23; SIX1; TIP39

UniProt ID: (Human) Q15475, (Mouse) Q62231

Entrez Gene ID: (Human) 6495, (Mouse) 20471

Molecular Function: homeodomain transcription factor

negative regulation of transcription from RNA polymerase II promoter ureteric bud development branching involved in ureteric bud morphogenesis organ induction kidney development outflow tract morphogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter apoptotic process pattern specification process skeletal muscle tissue development sensory perception of sound cell proliferation regulation of synaptic growth at neuromuscular junction regulation of skeletal muscle satellite cell proliferation facial nerve morphogenesis epithelial cell differentiation thyroid gland development olfactory placode formation regulation of protein localization protein localization to nucleus aorta morphogenesis inner ear morphogenesis middle ear morphogenesis negative regulation of neuron apoptotic process regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter thymus development neuron fate specification generation of neurons embryonic cranial skeleton morphogenesis embryonic skeletal system morphogenesis inner ear development regulation of epithelial cell proliferation myoblast migration pharyngeal system development myotome development fungiform papilla morphogenesis trigeminal ganglion development otic vesicle development metanephric mesenchyme development regulation of branch elongation involved in ureteric bud branching positive regulation of ureteric bud formation mesonephric tubule formation ureter smooth muscle cell differentiation positive regulation of secondary heart field cardioblast proliferation cochlea morphogenesis positive regulation of branching involved in ureteric bud morphogenesis negative regulation of branching involved in ureteric bud morphogenesis positive regulation of mesenchymal cell proliferation involved in ureter development regulation of skeletal muscle cell differentiation transcription, DNA-templated multicellular organism development regulation of gene expression neurogenesis negative regulation of apoptotic process tongue development skeletal system morphogenesis anatomical structure development renal system development

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding transcription regulatory region DNA binding