Proteintech

SIX2 Polyclonal Antibody

View all (5) SIX2 antibodies

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SIX2 Antibody in Immunofluorescence (IF) — IF result from Fogelgren B, PMID:18570229, "Misexpression of Six2 is associated with heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice.".

SIX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 11562-1-AP (SIX2 antibody) at dilution of 1:50 (under 10x lens).

SIX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 11562-1-AP (SIX2 antibody) at dilution of 1:50 (under 40x lens).

SIX2 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 11562-1-AP ( SIX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

SIX2 Antibody in Western Blot (WB) — L02 cells were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SIX2 Antibody in Western Blot (WB) — PC-3 cells were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

SIX2 Antibody in Immunoprecipitation (IP) — IP result of anti-SIX2 (IP:11562-1-AP, 3ug; Detection:11562-1-AP 1:500) with HEK-293 cells lysate 2700ug.

Product Details

Tested Applications

Dilution

ChIP assay (ChIP)

Assay-dependent

Immunofluorescence (IF)

Assay-Dependent

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:1000

Published Applications

Immunohistochemistry (IHC)

See 24 publications below

Immunofluorescence (IF)

See 97 publications below

Western Blot (WB)

See 17 publications below

Miscellaneous PubMed (MISC)

See 2 publications below

Immunoprecipitation (IP)

See 1 publication below

ChIP assay (ChIP)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat, Reptile

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SIX2 Fusion Protein Ag2124

Conjugate

Unconjugated

Form

Liquid

Concentration

0.52 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.

Target Information

This gene is a member of the vertebrate gene family which encode proteins homologous to the Drosophila 'sine oculis' homeobox protein. SIX2 is a transcription factor which, like other members of this gene family, may be involved in limb or eye development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Homeobox protein SIX2; Sine oculis homeobox homolog 2; sine oculis-related homeobox 2 homolog; SIX homeobox 2; SIX2

Gene Aliases: SIX2

UniProt ID: (Human) Q9NPC8, (Mouse) Q62232

Entrez Gene ID: (Human) 10736, (Mouse) 20472, (Rat) 366542

Molecular Function: helix-turn-helix transcription factor homeobox transcription factor transcription factor

kidney development chondrocyte differentiation mesenchymal to epithelial transition involved in metanephros morphogenesis transcription from RNA polymerase II promoter protein import into nucleus mesodermal cell fate specification cell proliferation anatomical structure morphogenesis anterior/posterior axis specification cell migration regulation of ossification regulation of chondrocyte differentiation middle ear morphogenesis negative regulation of cell differentiation positive regulation of transcription from RNA polymerase II promoter embryonic digestive tract morphogenesis embryonic cranial skeleton morphogenesis nephron development nephron morphogenesis mesenchymal stem cell maintenance involved in nephron morphogenesis mesenchymal cell differentiation involved in kidney development regulation of branching involved in ureteric bud morphogenesis mesenchymal stem cell proliferation positive regulation of chondrocyte proliferation metanephros development regulation of transcription, DNA-templated multicellular organism development embryonic skeletal system morphogenesis

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor binding protein complex binding DNA binding protein binding sequence-specific DNA binding