Western blot of SLAMF8 in HEK293 lysate overexpressing human SLAMF8 with C-terminal MYC tag was performed using 10ug protein in RIPA buffer. Lysates were probed with a SLAMF8 polyclonal antibody (Product# PA5-37955) at a dilution of 1ug/ml (Lane A) and with a MYC Tag antibody at a dilution of 1:1000 (Lane C). Lane B shows mock-transfected HEK293 cells probed with PA5-37955 at a dilution of 1ug/ml. All lysates were incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-TLYHSRFLGRAQ, from the internal region of SLAMF8|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse, rat and cow based on sequence homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.