Invitrogen

SLC26A4 Polyclonal Antibody

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SLC26A4 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of SLC26A4 in HepG2 cells using a SLC26A4 polyclonal antibody (Product # PA5-98158) at a dilution of 1:166. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

SLC26A4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of SLC26A4 in paraffin embedded human melanoma using a SLC26A4 polyclonal antibody (Product # PA5-98158) at a dilution of 1:500. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

SLC26A4 Antibody in Western Blot (WB) — Western Blot analysis of SLC26A4 using a SLC26A4 Polyclonal antibody (Product # PA5-98158) at a concentration of 5.7 µg/mL. Positive WB detected in: U87 whole cell lysate, SH-SY5Y whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, Colo320 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 86 kDa.

Product Details

Tested Applications

Dilution

ELISA (ELISA)

Assay-Dependent

Immunocytochemistry (ICC)

1:50-1:200

Immunofluorescence (IF)

1:50-1:200

Immunohistochemistry (IHC)

1:500-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Western Blot (WB)

1:500-1:5000

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human Pendrin protein (592-655AA)

Conjugate

Unconjugated

Form

Liquid

Concentration

4.3 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.03% Proclin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Target Information

Mutations in this gene are associated with Pendred syndrome, the most common form of syndromic deafness, an autosomal-recessive disease. It is highly homologous to the SLC26A3 gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Pendrin; Sodium-independent chloride/iodide transporter; solute carrier family 26 (anion exchanger), member 4; Solute carrier family 26 member 4; truncated solute carrier family 26

Gene Aliases: DFNB4; EVA; PDS; SLC26A4; TDH2B

UniProt ID: (Human) O43511

Entrez Gene ID: (Human) 5172

Function(s)