Invitrogen

SLC26A4 Polyclonal Antibody

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SLC26A4 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of SLC26A4 in HepG2 cells using a SLC26A4 polyclonal antibody (Product # PA5-98158) at a dilution of 1:166. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

SLC26A4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of SLC26A4 in paraffin embedded human melanoma using a SLC26A4 polyclonal antibody (Product # PA5-98158) at a dilution of 1:500. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

SLC26A4 Antibody in Western Blot (WB) — Western Blot analysis of SLC26A4 using a SLC26A4 Polyclonal antibody (Product # PA5-98158) at a concentration of 5.7 µg/mL. Positive WB detected in: U87 whole cell lysate, SH-SY5Y whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, Colo320 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 86 kDa.

Product Details

PA5-98158

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:5,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:500-1:1,000	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
ELISA (ELISA)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant Human Pendrin protein (592-655AA) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	4.31 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.03% Proclin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2812772

Target Information

Mutations in this gene are associated with Pendred syndrome, the most common form of syndromic deafness, an autosomal-recessive disease. It is highly homologous to the SLC26A3 gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Pendrin; Sodium-independent chloride/iodide transporter; solute carrier family 26 (anion exchanger), member 4; Solute carrier family 26 member 4; truncated solute carrier family 26

Gene Aliases: DFNB4; EVA; PDS; SLC26A4; TDH2B

UniProt ID: (Human) O43511

Entrez Gene ID: (Human) 5172

Molecular Function: transporter