Invitrogen

SLC2A6 Polyclonal Antibody

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SLC2A6 Antibody in Western Blot (WB) — Western blot analysis of SLC2A6 in the following samples: Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat lung tissue lysates. Samples consisting of 50 µg (reducing conditions) of protein was separated with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V, Resolving gel; 2-3 hrs.), transferred to a Nitrocellulose membrane (150mA, 50-90 min) and washed with TBS-0.1% Tween (3 times, 5 minutes/wash) and blocked with 5% Non-fat Milk/TBS (1.5 hrs., room temperature). The membrane was incubated in SLC2A6 polyclonal antibody (Product # PA5-114399) at a dilution of 0.5 µg/mL (overnight, 4°C), followed by goat anti-rabbit IgG-HRP and chemiluminescence (ECL) with a dilution of 1:10,000 (1.5 hours, room temperature).

Product Details

PA5-114399

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-0.5 µg/mL	-
ELISA (ELISA)	0.1-0.5 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E.coli-derived human GLUT6/SLC2A6 recombinant protein (Position: M1-R507). View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	500 µg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 4mg trehalose
Contains	0.05mg sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2884855

Product Specific Information

Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Target Information

Hexose transport into mammalian cells is catalyzed by a family of membrane proteins, including SLC2A6, that contain 12 transmembrane domains and a number of critical conserved residues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Glucose transporter type 6; glucose transporter type 9; GLUT-6; GLUT-9; solute carrier family 2 (facilitated glucose transporter), member 6; Solute carrier family 2, facilitated glucose transporter member 6

Gene Aliases: A330096C23; F630103L12Rik; GLUT6; GLUT9; HSA011372; SLC2A6

UniProt ID: (Human) Q5SXD7, (Mouse) Q3UDF0

Entrez Gene ID: (Human) 11182, (Rat) 296600, (Mouse) 227659

Function(s)

proton transport glucose import glucose transmembrane transport carbohydrate transport transmembrane transport hexose transmembrane transport cation transmembrane transport

Process(es)

sugar:proton symporter activity glucose transmembrane transporter activity D-glucose transmembrane transporter activity substrate-specific transmembrane transporter activity molecular_function