Invitrogen

SLC39A6 Polyclonal Antibody

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SLC39A6 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of SLC39A6 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and SLC39A6 polyclonal antibody (Product # PA5-100155) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

SLC39A6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded SLC39A6 in human pancreas cancer tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with SLC39A6 polyclonal antibody (Product # PA5-100155) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

SLC39A6 Antibody in Western Blot (WB) — Western blot analysis of SLC39A6 in 3T3 cell lysate (left lane: treated with blocking peptide). Samples were incubated with SLC39A6 polyclonal antibody (Product # PA5-100155).

Product Details

PA5-100155

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human SLC39A6(Accession Q13433), corresponding to amino acid residues Y531-H558. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2815685

Target Information

This gene encodes a protein which interacts with the carboxy-terminus of transcription initiation factor TFIIF, a transcription factor which regulates elongation as well as initiation by RNA polymerase II. The protein may also represent a component of an RNA polymerase II holoenzyme complex. Alternative splicing of this gene results in two transcript variants encoding 2 different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: endoplasmic reticulum membrane protein; Endoplasmic reticulum membrane-linked protein; Ermelin; Estrogen-regulated protein LIV-1; LIV-1 protein, estrogen regulated; slc39a6 {ECO:0000312|EMBL:AAH97493.1}; solute carrier family 39 (metal ion transporter), member 6; solute carrier family 39 (zinc transporter), member 6; Solute carrier family 39 member 6; Zinc transporter ZIP6; ZIP-6; Zrt- and Irt-like protein 6

Gene Aliases: Ermelin; LIV-1; LIV1; SLC39A6; ZIP6

UniProt ID: (Human) Q13433, (Mouse) Q8C145, (Rat) Q4V887

Entrez Gene ID: (Human) 25800, (Mouse) 106957, (Rat) 291733

Molecular Function: secondary carrier transporter