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          • Primary Antibodies ›
          • SLC5A7 Antibodies

          Invitrogen

          SLC5A7 Polyclonal Antibody

          View all (5) SLC5A7 antibodies

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          Cite SLC5A7 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          SLC5A7 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          SLC5A7 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          SLC5A7 Antibody (PA5-143934) in WB

          Western blot analysis of SLC5A7 using SLC5A7 antibody (Product # PA5-143934). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human A549 whole cell lysates; Lane 2: human RT4 whole cell lysates; Lane 3: rat C6 whole cell lysates; Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Block... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SLC5A7 Antibody in Western Blot (WB)
          SLC5A7 Antibody in Flow Cytometry (Flow)
          SLC5A7 Polyclonal Antibody

          Product Details

          PA5-143934

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.25-0.5 µg/mL
          -

          Flow Cytometry (Flow)

          1-3 µg/mL/1x10^6 cells
          -

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived human SLC5A7 recombinant protein (Position: R446-Q580).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3075148

          Product Specific Information

          Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

          Positive Control - WB: human A549 whole cell, humna RT4 whle cell, rat C6 whole cell, mouse NIH/3T3 whole cell. Flow: JK cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          Acetylcholine functions as a neurotransmitter in both the central and peripheral nervous systems of all vertebates, and is the principle neurotransmitter used at the neuromuscular junction. Presynaptic synthesis of acetylcholine requires a steady supply of choline, which is acquired by a plasma membrane high affinity choline transporter (CHT).

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: CHT; hCHT; hCHT1; Hemicholinium-3-sensitive choline transporter; High affinity choline transporter 1; high affinity choline transporter; hemicholinium-3-sensitive choline transporter; MGC126299; MGC126300; Motor neuronopathy, distal hereditary, type VIIA; Motor neuronopathy, distal hereditary, with vocal cord paresis; rCHT1; solute carrier family 5 (choline transporter), member 7; solute carrier family 5 (sodium/choline cotransporter), member 7; Solute carrier family 5 member 7; solute carrier family 5, member 7; unnamed protein product

          View more View less

          Gene Aliases: CHT; CHT1; CMS20; DHMNVP; hCHT1; HMN7A; HMND7; SLC5A7

          View more View less

          UniProt ID: (Human) Q9GZV3, (Mouse) Q8BGY9, (Rat) Q9JMD7

          View more View less

          Entrez Gene ID: (Human) 60482, (Mouse) 63993, (Rat) 85426

          View more View less

          Function(s)
          choline:sodium symporter activity choline transmembrane transporter activity symporter activity transmembrane transporter activity choline binding transporter activity protein binding transporter
          Process(es)
          in utero embryonic development ion transport sodium ion transport neurotransmitter transport synaptic transmission, cholinergic neuromuscular synaptic transmission acetylcholine biosynthetic process choline transport transmembrane transport transport organic cation transport sodium ion transmembrane transport neurotransmitter biosynthetic process ammonium transmembrane transport cation transmembrane transport
          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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