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Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
10-50 µg/mL | - |
Immunohistochemistry (IHC) |
10-50 µg/mL | - |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
A synthetic peptide from cytoplasmic domain of Sodium / Hydrogen Exchanger 1 (SLC9A1, NHE1) conjugated to an immunogenic carrier protein was used as the antigen |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Concentration |
Conc. Not Determined |
Purification |
Ammonium sulfate precipitation |
Storage buffer |
PBS |
Contains |
no preservative |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: SL9A1.
The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: APNH; Na(+)/H(+) antiporter, amiloride-sensitive; Na(+)/H(+) exchanger 1; Na-Li countertransporter; NHE-1; protein phosphatase 1, regulatory subunit 143; Sodium/hydrogen exchanger 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter, Na+/H+, amiloride sensitive); solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive); Solute carrier family 9 member 1; solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1
Gene Aliases: APNH; APNH1; LIKNS; NHE-1; NHE1; PPP1R143; SLC9A1
UniProt ID: (Human) P19634
Entrez Gene ID: (Human) 6548
Molecular Function:
transporter
secondary carrier transporter
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