Rockland

SLIT2 Polyclonal Antibody

View all (12) SLIT2 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

600-401-846

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500- 1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

2 µg/mL
-

ELISA (ELISA)

1:5,000-1:50,000
-

Immunoprecipitation (IP)

1:500-1:2,000
-
Product Specifications

Species Reactivity

Chicken, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 475-500 of Human SLIT-2 protein.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against human SLIT-2 protein. A BLAST analysis was used to suggest reactivity with this protein from human and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-2 from Tetraodon nigroviridis , as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). The core 13 amino acids of the immunogen are also 100% homologous to SLIT-2 from mouse, rat, bovine and Xenopus laevis. Cross reactivity with SLIT-2 homologues from other sources has not been determined.

Target Information

SLIT2 are ligands for Robo receptors and play a vital role for axonal migration/navigation during neural development. A number of cleavage products through alternate splicing are reported in the literature for SLIT2 proteins. The C-terminal cleavage proteins are more diffusible than the larger N-terminal protein that is more tightly cell associated. SLIT2 protein is expressed in tissues such as fetal lung, kidney, and adult spinal cord.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: Slit homolog 2 protein; Slit-2

View more View less

Gene Aliases: SLIL3; Slit; Slit-2; SLIT2

View more View less

UniProt ID: (Human) O94813

View more View less

Entrez Gene ID: (Human) 9353, (Chicken) 373967

View more View less

Function(s)
GTPase inhibitor activity calcium ion binding protein binding heparin binding identical protein binding protein homodimerization activity laminin-1 binding proteoglycan binding heparan sulfate proteoglycan binding Roundabout binding immunoglobulin superfamily cell adhesion molecule
Process(es)
ureteric bud development negative regulation of protein phosphorylation cell migration involved in sprouting angiogenesis negative regulation of leukocyte chemotaxis axon guidance motor neuron axon guidance negative regulation of lamellipodium assembly negative regulation of endothelial cell migration negative regulation of smooth muscle cell migration spinal cord development chemorepulsion involved in embryonic olfactory bulb interneuron precursor migration chemorepulsion involved in postnatal olfactory bulb interneuron migration corticospinal neuron axon guidance through spinal cord negative regulation of cell growth negative regulation of cell migration negative regulation of actin filament polymerization retinal ganglion cell axon guidance response to nutrient levels cellular response to hormone stimulus negative regulation of GTPase activity Roundabout signaling pathway positive regulation of apoptotic process negative regulation of vascular permeability branching morphogenesis of an epithelial tube axon extension involved in axon guidance negative regulation of inflammatory response positive regulation of axonogenesis negative chemotaxis induction of negative chemotaxis negative regulation of small GTPase mediated signal transduction response to cortisol apoptotic process involved in luteolysis negative regulation of chemokine-mediated signaling pathway cellular response to heparin negative regulation of smooth muscle cell chemotaxis negative regulation of mononuclear cell migration negative regulation of neutrophil chemotaxis negative regulation of monocyte chemotaxis negative regulation of retinal ganglion cell axon guidance negative regulation of cellular response to growth factor stimulus
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support