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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide near C-terminus of human SLIT2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is prostate adenocarcinoma or fetal kidney.
Slit homolog 2 proteins (SLIT2) are ligands for the Roundabout (Robo) receptors and act as molecule guidance cues in cell migration. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. Slit2 protein is expressed in fetal lung and kidney, and adult spinal cord. Weak expression is also noticed in adult adrenal gland, thyroid, and trachea. SLIT2 is a target for repression by EZH2 and is up-regulated in prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
SLIL3; slit homolog 2 protein
SLIL3; Slit-2; SLIT2