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Invitrogen
SLIT3 Polyclonal Antibody
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FIGURE: 1 / 6
SLIT3 Antibody (PA5-104142) in ICC/IF
Immunofluorescent analysis of SLIT3 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and SLIT3 polyclonal antibody (Product # PA5-104142) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-104142
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SLIT3(Accession O75094), corresponding to amino acid residues K1260-R1310.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total SLIT3.
Target Information
Slit guidance ligand 3 (SLIT3) is a gene located on chromosome 5q35.1, encoding a member of the SLIT family of proteins that play key roles in axon guidance and neuronal migration during development. SLIT3 functions as a secreted protein, influencing the directional growth and pathfinding of axons by interacting with its receptor, Robo, on the surface of neurons. This interaction is critical for establishing neural network architecture and maintaining proper neural connectivity. Beyond its role in the nervous system, SLIT3 is involved in processes such as angiogenesis and cell migration, impacting tissue development and repair. Dysregulation of SLIT3 expression or function has been associated with developmental abnormalities and various diseases, including cancer, where it may influence tumor cell invasion and metastasis. Research into SLIT3 continues to explore its therapeutic potential in modulating axon guidance and its implications in pathological conditions affecting both the nervous and vascular systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: multiple EGF like domains 5; Multiple EGF-like domains protein 5; multiple epidermal growth factor-like domains 5; Multiple epidermal growth factor-like domains protein 5; SLIL2FLJ10764; slit homolog 3; Slit homolog 3 protein; Slit-3
Gene Aliases: b2b2362.1Clo; KIAA0814; MEGF5; SLIL2; Slit-3; SLIT1; slit2; SLIT3; UNQ691/PRO1336
UniProt ID: (Human) O75094, (Rat) O88280, (Mouse) Q9WVB4
Entrez Gene ID: (Human) 6586, (Rat) 83467, (Mouse) 20564
axon guidance
brain development
negative regulation of cell proliferation
organ morphogenesis
negative regulation of gene expression
spinal cord development
chemorepulsion involved in embryonic olfactory bulb interneuron precursor migration
cell differentiation
negative regulation of cell growth
cellular response to hormone stimulus
Roundabout signaling pathway
axon extension involved in axon guidance
negative chemotaxis
response to cortisol
apoptotic process involved in luteolysis
negative regulation of chemokine-mediated signaling pathway
multicellular organism development
nervous system development
aortic valve morphogenesis
atrioventricular valve morphogenesis
ventricular septum morphogenesis
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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