Invitrogen

SLUG Polyclonal Antibody

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SLUG Antibody — Figure 3 Calreticulin Absence Reduces S9 Phosphorylation of GSK3beta and Affects SNAIL2/SLUG Nuclear Translocation (A) qPCR analysis shows the mRNA expression of TGF-beta receptor markers TgfbR1 , TgfbR2 , and TgfbR3 in WT and CRT-KO cells during differentiation. (B) N-cadherin, E-cadherin, pAKT (S473), and pGSK3beta (S9) versus total GSK3beta and total AKT were examined in WT, CRT-KO, and CN cells at D14 of cardiac differentiation from total cell lysates by western blot analysis. Compared with calreticulin-containing WT cells, in KO cells E-cadherin is more abundant, while in contrast N-cadherin is more abundant in WT cells than in calreticulin-deficient KO cells. CN cells express less E-cadherin and more N-cadherin. AKT phosphorylation at serine 473 and GSK3beta phosphorylation at S9 is low in CRT-KO cells. The bar graphs show the quantification of E-cad/N-cad, pAKT, and pGSK3beta band density versus GAPDH from three independent experiments; * p <= 0.05, ** p <= 0.01, *** p <= 0.001, **** p <= 0.0001. (C) Western blot detection of SNAIL2/SLUG in nuclear fractions of in WT, CRT-KO, and CN cells during cardiac differentiation in D10 and D14.

SLUG Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of human kidney using a SLUG polyclonal antibody (Product # PA1-86737).

SLUG Antibody in Western Blot (WB) — Western blot analysis of whole cell lysate from HEK293 human epithelial kidney cells probed with a SLUG polyclonal antibody (Product # PA1-86737) at 0.5 (A), and 1 (B) and 2 (C) µg/mL

Product Details

Tested Applications

Dilution

Immunofluorescence (IF)

Assay-dependent

Western Blot (WB)

0.5-2 µg/mL

Published Applications

Western Blot (WB)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

13 amino acid peptide sequence near the centre of human Slug

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4°C or -20°C if preferred

RRID

AB_2302174

Product Specific Information

In Western blot, this antibody detects a band at ~30kDa in 293 cell lysate.

Target Information

This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: MGC10182; Neural crest transcription factor Slug; Protein snail homolog 2; SLUG; slug (chicken homolog), zinc finger protein; slug, chicken homolog; SLUGH1; snail family zinc finger 2; snail homolog 2; WS2D; Zinc finger protein SNAI2

Gene Aliases: SLUG; SLUGH; SLUGH1; SNAI2; SNAIL2; WS2D

UniProt ID: (Human) O43623, (Mouse) P97469

Entrez Gene ID: (Human) 6591, (Mouse) 20583

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation epithelial to mesenchymal transition epithelial to mesenchymal transition involved in endocardial cushion formation cell migration involved in endocardial cushion formation transcription, DNA-templated negative regulation of cell adhesion involved in substrate-bound cell migration Notch signaling pathway sensory perception of sound negative regulation of keratinocyte proliferation negative regulation of vitamin D biosynthetic process neural crest cell development positive regulation of cell migration negative regulation of chondrocyte differentiation regulation of chemokine production negative regulation of cell adhesion mediated by integrin positive regulation of histone acetylation negative regulation of catenin import into nucleus desmosome disassembly pigmentation negative regulation of DNA damage response, signal transduction by p53 class mediator cellular response to fibroblast growth factor stimulus positive regulation of fat cell differentiation regulation of osteoblast differentiation white fat cell differentiation palate development canonical Wnt signaling pathway epithelium development cartilage morphogenesis regulation of branching involved in salivary gland morphogenesis negative regulation of vitamin D receptor signaling pathway cellular response to epidermal growth factor stimulus cellular response to ionizing radiation negative regulation of canonical Wnt signaling pathway negative regulation of cell-cell adhesion by negative regulation of transcription from RNA polymerase II promoter negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of stem cell proliferation regulation of bicellular tight junction assembly negative regulation of anoikis negative regulation of extrinsic apoptotic signaling pathway in absence of ligand regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development response to radiation cell migration cellular response to platelet-derived growth factor stimulus regulation of apoptotic process negative regulation of apoptotic process wound healing, spreading of cells positive regulation of endothelial cell chemotaxis

Process(es)

transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding sequence-specific DNA binding metal ion binding nucleic acid binding DNA binding transcription factor activity, sequence-specific DNA binding

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