Invitrogen

SLUG Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Reference

View all (45) SLUG antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

SLUG Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of SLUG was performed using 70% confluent log phase MCF-7 cells treated with 10 ng/mL TGF-Beta for 4 days. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled Anti-SLUG Polyclonal Antibody (Product # PA5-20289) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27034) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents untreated MCF-7 cells having low expression of SLUG. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

SLUG Antibody in Western Blot (WB) — Western blot was performed using Anti-SLUG Polyclonal Antibody (Product # PA5-20289) and a 29kDa band corresponding to Slug was observed across all the cell lines tested and increased upon TGF-Beta treatment in MCF7. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), MDA-MB-231 (Lane 2), MCF-7 (Lane 3), MCF-7 treated with TGF-Beta (10ng/ml for 4 days (Lane 4), MCF 10A (Lane 5), U-937 (Lane 6) and Daudi were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

SLUG Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of Slug was observed in U-87 MG, MDA-MB-231, MCF-7 and MCF 10A cell lines in comparison to U-937 and Daudi cell lines which express Slug protein at lower levels using Anti-SLUG Polyclonal Antibody (Product # PA5-20289) in Western Blot

Product Details

PA5-20289

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 14 amino acid peptide from near the center of human Slug.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

RRID

AB_11156388

Product Specific Information

A suggested positive control is human kidney tissue lysate.

PA5-20289 can be used with blocking peptide PEP-0419.

Target Information

Slug, a member of the Snail family of C2H2-type zinc finger transcription factors, was initially identified to be involved in epithelial-mesenchymal transitions as well as the formation of the neural tube during vertebrate embryogenesis. Like Snail, Slug transcription can be induced by growth factors such as FGF, BMP, and TGF-beta. Once expressed, Slug will bind E-box regions of promoters and repress transcription of genes such as E-cadherin and Claudin-1. More recently, its expression in breast, esophogeal, and colorectal carcinomas has been correlated with poor prognosis for survival. Furthermore, Slug can protect hemopoietic progenitor cells from radiation-induced apoptosis by repressing the p53-mediated transcription of Puma, a BH3-only antagonist of the anti-apoptotic members of the Bcl-2 family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Parathyroid Hormone-Related Protein Inhibition Blocks Triple-Negative Breast Cancer Expansion in Bone Through Epithelial to Mesenchymal Transition Reversal.

JBMR plus

Li J,Camirand A,Zakikhani M,Sellin K,Guo Y,Luan X,Mihalcioiu C,Kremer R

PA5-20289 was used in Immunocytochemistry-immunoflourescence to conclude that PTHrP is a targetable EMT molecular driver and suggest that its pharmacological blockade can provide a potential therapeutic approach against established TNBC-derived skeletal lesions.

Wed Jun 01 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: MGC10182; Neural crest transcription factor Slug; Protein snail homolog 2; slug (chicken homolog), zinc finger protein; slug, chicken homolog; snail family zinc finger 2; snail homolog 2; Zinc finger protein SNAI2

Gene Aliases: SLUG; SLUGH; SLUGH1; SNAI2; SNAIL2; WS2D

UniProt ID: (Human) O43623, (Mouse) P97469

Entrez Gene ID: (Human) 6591, (Mouse) 20583

Molecular Function: C2H2 zinc finger transcription factor