Invitrogen

SLUG Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

3 References

View all (45) SLUG antibodies

Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

Product Image Gallery

SLUG Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of SLUG was performed using 70% confluent log phase MCF-7 cells treated with 10 ng/mL TGF-Beta for 4 days. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled Anti-SLUG Polyclonal Antibody (Product # Anti-SLUG Polyclonal Antibody (Product # PA5-73015) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27034) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents untreated MCF-7 cells having low expression of SLUG. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

SLUG Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of SLUG in gastric cancer. Samples were incubated in SLUG polyclonal antibody (Product # PA5-73015) using a dilution of 1:25. Image objective 400X.

SLUG Antibody in Western Blot (WB) — Western blot was performed using Anti-SLUG Polyclonal Antibody (Product # PA5-73015) and a 29kDa band corresponding to Slug was observed across all the cell lines tested and increased upon TGF-Beta treatment in MCF7. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), MDA-MB-231 (Lane 2), MCF-7 (Lane 3), MCF-7 treated with TGF-Beta (10ng/ml for 4 days) (Lane 4), MCF 10A (Lane 5), U-937 (Lane 6) and Daudi were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

SLUG Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig. 3 Overexpression of SNAI2 in IM cells. (a) GES-1 cells were treated as in Fig. 1b . The protein level of SNAI2 was detected by Western blotting (Left) and the results were normalized to beta-actin (Right). (b) SNAI2 mRNA levels examined in GES-1 cells after DCA treatment by qRT-PCR. (c, d) Primary cultured cells were treated with DCA as for GES-1 cells, after which SNAI2 was detected at protein and mRNA levels, respectively. (e) Representative pictures of IHC staining for SNAI2 in normal and gastric IM tissues. Scale bars: 100 mum and 500 mum (insets). (f) Quantification of IHC staining for SNAI2. (g) Correlation between SNAI2 and FXR expression in IM specimens. (h) SNAI2 mRNA levels in 10 pairs of matched human IM/normal specimens. * p < 0.05; ** p < 0.01

SLUG Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of Slug was observed in U-87 MG, MDA-MB-231, MCF-7 and MCF 10A cell lines in comparison to U-937 and Daudi cell lines which express Slug protein at lower levels using Anti-Slug Polyclonal Antibody (Product # PA5-73015) in Western Blot.

Product Details

PA5-73015

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Dog, Horse, Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Amino acids 82-110 of human SNAIL2 (SNAI2) were used as the immunogen.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2718869

Target Information

Slug, a member of the Snail family of C2H2-type zinc finger transcription factors, was initially identified to be involved in epithelial-mesenchymal transitions as well as the formation of the neural tube during vertebrate embryogenesis. Like Snail, Slug transcription can be induced by growth factors such as FGF, BMP, and TGF-beta. Once expressed, Slug will bind E-box regions of promoters and repress transcription of genes such as E-cadherin and Claudin-1. More recently, its expression in breast, esophogeal, and colorectal carcinomas has been correlated with poor prognosis for survival. Furthermore, Slug can protect hemopoietic progenitor cells from radiation-induced apoptosis by repressing the p53-mediated transcription of Puma, a BH3-only antagonist of the anti-apoptotic members of the Bcl-2 family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Bile acids increase intestinal marker expression via the FXR/SNAI2/miR-1 axis in the stomach.

Cellular oncology (Dordrecht)

Wang N,Wu S,Zhao J,Chen M,Zeng J,Lu G,Wang J,Zhang J,Liu J,Shi Y

PA5-73015 was used in Immunohistochemistry (Paraffin), Immunohistochemistry to investigate the mechanism underlying miR-1 inhibition by bile acid in gastric cells.

Fri Oct 01 00:00:00 EDT 2021

Bile acids increase intestinal marker expression via the FXR/SNAI2/miR-1 axis in the stomach.

Cellular oncology (Dordrecht)

Wang N,Wu S,Zhao J,Chen M,Zeng J,Lu G,Wang J,Zhang J,Liu J,Shi Y

PA5-73015 was used in Immunohistochemistry (Paraffin), Immunohistochemistry to investigate the mechanism underlying miR-1 inhibition by bile acid in gastric cells.

Fri Oct 01 00:00:00 EDT 2021

Bile acids increase intestinal marker expression via the FXR/SNAI2/miR-1 axis in the stomach.

Cellular oncology (Dordrecht)

Wang N,Wu S,Zhao J,Chen M,Zeng J,Lu G,Wang J,Zhang J,Liu J,Shi Y

PA5-73015 was used in Immunohistochemistry (Paraffin), Immunohistochemistry to investigate the mechanism underlying miR-1 inhibition by bile acid in gastric cells.

Fri Oct 01 00:00:00 EDT 2021

The metastasis suppressor, NDRG1, attenuates oncogenic TGF-β and NF-κB signaling to enhance membrane E-cadherin expression in pancreatic cancer cells.

Carcinogenesis

Menezes SV,Fouani L,Huang MLH,Geleta B,Maleki S,Richardson A,Richardson DR,Kovacevic Z

PA5-73015 was used in Immunohistochemistry to study how N-myc downstream-regulated gene-1 inhibits oncogenic effects of transforming growth factor-β in PANC-1 pancreatic cancer cells, promoting expression and co-localization of E-cadherin and β-catenin at the cell membrane.

Sat Jul 06 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: MGC10182; Neural crest transcription factor Slug; Protein snail homolog 2; slug (chicken homolog), zinc finger protein; slug, chicken homolog; snail family zinc finger 2; snail homolog 2; snail-like protein 2; Zinc finger protein SNAI2; zinc finger transcription factor snail 2

Gene Aliases: SLUG; SLUGH; SNAI2

SLUG Polyclonal Antibody

Product Details

PA5-73015

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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