Invitrogen

SLUG Polyclonal Antibody

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SLUG Antibody in Immunohistochemistry (IHC) — Immunohistochemistry was performed on liver cancer tissue using a Slug polyclonal primary antibody (Product # PA5-73015) at a dilution of 1:25. Images were taken at 400x magnification.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Western Blot (WB)

1:2000

Product Specifications

Species Reactivity

Dog, Horse, Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Amino acids 82-110 of human SNAIL2 (SNAI2) were used as the immunogen.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2718869

Target Information

Slug, a member of the Snail family of C2H2-type zinc finger transcription factors, was initially identified to be involved in epithelial-mesenchymal transitions as well as the formation of the neural tube during vertebrate embryogenesis. Like Snail, Slug transcription can be induced by growth factors such as FGF, BMP, and TGF-beta. Once expressed, Slug will bind E-box regions of promoters and repress transcription of genes such as E-cadherin and Claudin-1. More recently, its expression in breast, esophogeal, and colorectal carcinomas has been correlated with poor prognosis for survival. Furthermore, Slug can protect hemapoietic progenitor cells from radiation-induced apoptosis by repressing the p53-mediated transcription of Puma, a BH3-only antagonist of the anti-apoptotic members of the Bcl-2 family. Slug antibody has no cross-reactivity to Snail protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Neural crest transcription factor Slug; Protein snail homolog 2; slug (chicken homolog), zinc finger protein; slug, chicken homolog; SLUG, SLUGH1, SNAIL2, WS2D; snail family zinc finger 2; snail homolog 2; Zinc finger protein SNAI2; zinc finger transcription factor snail 2

Gene Aliases: SLUG; SLUGH; SLUGH1; SNAI2; SNAIL2; WS2D

UniProt ID: (Human) O43623, (Mouse) P97469

Entrez Gene ID: (Human) 6591, (Dog) 486940, (Mouse) 20583

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation epithelial to mesenchymal transition epithelial to mesenchymal transition involved in endocardial cushion formation cell migration involved in endocardial cushion formation transcription, DNA-templated negative regulation of cell adhesion involved in substrate-bound cell migration Notch signaling pathway sensory perception of sound negative regulation of keratinocyte proliferation negative regulation of vitamin D biosynthetic process neural crest cell development positive regulation of cell migration negative regulation of chondrocyte differentiation regulation of chemokine production negative regulation of cell adhesion mediated by integrin positive regulation of histone acetylation negative regulation of catenin import into nucleus desmosome disassembly pigmentation negative regulation of DNA damage response, signal transduction by p53 class mediator cellular response to fibroblast growth factor stimulus positive regulation of fat cell differentiation regulation of osteoblast differentiation white fat cell differentiation palate development canonical Wnt signaling pathway epithelium development cartilage morphogenesis regulation of branching involved in salivary gland morphogenesis negative regulation of vitamin D receptor signaling pathway cellular response to epidermal growth factor stimulus cellular response to ionizing radiation negative regulation of canonical Wnt signaling pathway negative regulation of cell-cell adhesion by negative regulation of transcription from RNA polymerase II promoter negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of stem cell proliferation regulation of bicellular tight junction assembly negative regulation of anoikis negative regulation of extrinsic apoptotic signaling pathway in absence of ligand regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development response to radiation cell migration cellular response to platelet-derived growth factor stimulus regulation of apoptotic process negative regulation of apoptotic process wound healing, spreading of cells positive regulation of endothelial cell chemotaxis

Process(es)

transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding sequence-specific DNA binding metal ion binding nucleic acid binding DNA binding transcription factor activity, sequence-specific DNA binding