Immunohistochemistry analysis of SMAD1 showing staining in the cytoplasm of paraffin-embedded mouse heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a SMAD1 Rabbit Polyclonal Antibody (385400) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Non-human primate|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from an internal region of the human Smad1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
SMAD1 belongs to the SMAD family of proteins, which are downstream effectors of the TGF beta/BMP signaling pathway. Members of the SMAD family transmit TGF- beta signals from the cell surface into the nucleus. Once in the nucleus, SMADs can target a variety of DNA binding proteins to regulate transcriptional responses. SMAD1 is a receptor-regulated SMAD in the cell to regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Pyruvate Dehydrogenase Kinase 4 Promotes Vascular Calcification via SMAD1/5/8 Phosphorylation.
38-5400 was used in immunocytochemistry to study how PDK4 contributes to vascular calcification
|Lee SJ,Jeong JY,Oh CJ,Park S,Kim JY,Kim HJ,Doo Kim N,Choi YK,Do JY,Go Y,Ha CM,Ha CM,Choi JY,Huh S,Ho Jeoung N,Lee KU,Choi HS,Wang Y,Park KG,Harris RA,Lee IK||Scientific reports (5:null)||2015|
Recurrent somatic mutations in ACVR1 in pediatric midline high-grade astrocytoma.
38-5400 was used in immunohistochemistry - paraffin section to identify therapeutic targets for pediatric midline high-grade astrocytomas.
|Fontebasso AM,Papillon-Cavanagh S,Schwartzentruber J,Nikbakht H,Gerges N,Fiset PO,Bechet D,Faury D,De Jay N,Ramkissoon LA,Corcoran A,Jones DT,Sturm D,Johann P,Tomita T,Goldman S,Nagib M,Bendel A,Goumnerova L,Bowers DC,Leonard JR,Rubin JB,Alden T,Browd S,Geyer JR,Leary S,Jallo G,Cohen K,Gupta N,Prados MD,Carret AS,Ellezam B,Crevier L,Klekner A,Bognar L,Hauser P,Garami M,Myseros J,Dong Z,Siegel PM,Malkin H,Ligon AH,Albrecht S,Pfister SM,Ligon KL,Majewski J,Jabado N,Kieran MW||Nature genetics (46:462)||2014|
Expression of GATA3 in MDA-MB-231 triple-negative breast cancer cells induces a growth inhibitory response to TGFß.
38-5400 was used in western blot to examine the relation of GATA3 and TGF beta in breast cancer cells.
|Chu IM,Lai WC,Aprelikova O,El Touny LH,Kouros-Mehr H,Green JE||PloS one (8:null)||2013|
Transforming growth factor ß inhibits bone morphogenetic protein-induced transcription through novel phosphorylated Smad1/5-Smad3 complexes.
38-5400 was used in western blot to study the relationship between bone morphogenetic protein and TGF-β.
|Grönroos E,Kingston IJ,Ramachandran A,Randall RA,Vizán P,Hill CS||Molecular and cellular biology (32:2904)||2012|
|Distinct modes of inhibition by sclerostin on bone morphogenetic protein and Wnt signaling pathways.||Krause C,Korchynskyi O,de Rooij K,Weidauer SE,de Gorter DJ,van Bezooijen RL,Hatsell S,Economides AN,Mueller TD,Löwik CW,ten Dijke P||The Journal of biological chemistry (285:41614)||2010|
|Not Applicable||Not Cited||
Growth/differentiation factor-15 inhibits differentiation into osteoclasts--a novel factor involved in control of osteoclast differentiation.
38-5400 was used in western blot to discuss the role of GDF-15 in modulation of osteoclast differentiation
|Vanhara P,Lincová E,Kozubík A,Jurdic P,Soucek K,Smarda J||Differentiation; research in biological diversity (78:213)||2009|
|Mouse||Not Cited||BMP7 and SHH regulate Pax2 in mouse retinal astrocytes by relieving TLX repression.||Sehgal R,Sheibani N,Rhodes SJ,Belecky Adams TL||Developmental biology (332:429)||2009|