Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
SMAD1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 2
SMAD1 Antibody (PA5-120107) in WB
Western blot analysis of SMAD1 in extracts of Jurkat cells. Samples were incubated with SMAD1 Polyclonal antibody (Product # PA5-120107) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Product Details
PA5-120107
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human Smad1 (NP_001003688.1).
Conjugate
Form
Liquid
Concentration
0.28 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: Jurkat, C6. The target is usually found in the following locations: Cytoplasm, Nucleus.
Immunogen sequence: WKQGDEEEKW AEKAVDALVK KLKKKKGAME ELEKALSCPG QPSNCVTIPR SLDGRLQVSH RKGLPHVIYC RVWRWPDLQS HHELKPLECC EFPFGSKQKE VCINPYHYKR VESPVLPPVL VPRHSEYNPQ HSLLAQFRNL GQNEPHMPLN ATFPDSFQQP NSHPFPHSPN SSYPNSPGSS SSTYPHSPTS SDPGSPFQMP ADTPPPAYLP PEDPMTQDGS Q
Target Information
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: BSP-1; DWFC; hSMAD1; JV4-1; JV5-1; MAD (mothers against decapentaplegic, Drosophila) homolog 1; MAD homolog 1; MAD homolog1 (mothers against decapentaplegic, Drosophila); MAD, mothers against decapentaplegic homolog 1; Mad-related protein 1; MADH5; Mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; Mothers-against-DPP-related-1; OTTHUMP00000220456; SMAD; SMAD 1; SMAD family member 1; SMAD, mothers against DPP homolog 1; SMAD5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1
Gene Aliases: BSP-1; BSP1; JV4-1; JV41; Mad1; MADH1; MADR1; SMAD1
UniProt ID: (Human) Q15797, (Rat) P97588
Entrez Gene ID: (Human) 4086, (Rat) 25671
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
receptor signaling protein activity
protein binding
protein kinase binding
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
identical protein binding
protein homodimerization activity
metal ion binding
protein heterodimerization activity
co-SMAD binding
I-SMAD binding
DNA-binding transcription factor
gene-specific transcriptional regulator
MAPK cascade
ureteric bud development
mesodermal cell fate commitment
osteoblast fate commitment
transcription from RNA polymerase II promoter
inflammatory response
signal transduction
transforming growth factor beta receptor signaling pathway
SMAD protein complex assembly
gamete generation
negative regulation of cell proliferation
embryonic pattern specification
positive regulation of gene expression
BMP signaling pathway
midbrain development
hindbrain development
primary miRNA processing
homeostatic process
positive regulation of osteoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
cartilage development
cardiac muscle cell proliferation
bone development
SMAD protein signal transduction
positive regulation of cartilage development
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
kidney development
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein phosphorylation
response to organonitrogen compound
negative regulation of muscle cell apoptotic process
wound healing
response to drug
positive regulation of cell differentiation
positive regulation of dendrite morphogenesis
cellular response to organic cyclic compound
cellular response to BMP stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support