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          • Primary Antibodies ›
          • SMAD3 Antibodies

          Rockland

          SMAD3 Polyclonal Antibody

          View all (71) SMAD3 antibodies

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          Cite SMAD3 Polyclonal Antibody

          Additional Information:

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          • Antibody Testing Data (2)
          SMAD3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          SMAD3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          SMAD3 Antibody (600-401-920) in IHC (P)

          Rocklands Affinity Purified anti-Smad3 antibody shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
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          SMAD3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SMAD3 Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          SMAD3 Polyclonal Antibody

          Product Details

          600-401-920

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:500-1:3,000
          -

          ELISA (ELISA)

          1:10,000-1:40,000
          -
          Product Specifications

          Species Reactivity

          Bovine, Chicken, Human, Mouse, Pig, Rat, Xenopus, Zebrafish

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of human SMAD3 protein.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.2 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

          Contains

          0.01% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

          SMAD3 affinity-purified antibody is directed against human Smad3 protein. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat, swine, and chicken sources based on 100% homology for the immunogen sequence. ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated human SMAD3.

          Target Information

          SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: DKFZP586N0721; DKFZp686J10186; MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 3; MAD homolog 3a; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; MAD-3; mad3; MGC60396; mMad3; mothers against decapentaplegic homolog 3; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD; SMAD 3; SMAD, mothers against DPP homolog 3; TGF-beta response effector Smad3; transcription factor; unnamed protein product

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          Gene Aliases: hMAD-3; hSMAD3; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; mad3; MADH3; madh3-A; madh3a; Smad 3; smad3; wu:fa99e03; XELAEV_18018454mg; XenMLP; Xmad3; XSmad3

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          UniProt ID: (Pig) P84024, (Chicken) P84023, (Human) P84022, (Mouse) Q8BUN5, (Rat) P84025

          View more View less

          Entrez Gene ID: (Pig) 397260, (Chicken) 395132, (Bovine) 515125, (Human) 4088, (Mouse) 17127, (Rat) 25631, (Xenopus) 378633, (Zebrafish) 58092

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          Function(s)
          core promoter proximal region sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding metal ion binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription cofactor activity transforming growth factor beta receptor binding collagen binding transcription factor binding zinc ion binding DEAD/H-box RNA helicase binding protein kinase binding phosphatase binding chromatin DNA binding ubiquitin protein ligase binding mineralocorticoid receptor binding glucocorticoid receptor binding protein homodimerization activity ubiquitin binding bHLH transcription factor binding co-SMAD binding I-SMAD binding R-SMAD binding primary miRNA binding transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding transcription corepressor binding DNA binding chromatin binding double-stranded DNA binding protein binding beta-catenin binding ligand-dependent nuclear receptor binding enzyme binding sterol response element binding identical protein binding sequence-specific DNA binding RNA polymerase II sequence-specific DNA binding transcription factor binding DNA-binding transcription factor binding promoter-specific chromatin binding DNA-binding transcription factor gene-specific transcriptional regulator
          Process(es)
          regulation of transcription, DNA-templated transforming growth factor beta receptor signaling pathway negative regulation of transcription from RNA polymerase II promoter ureteric bud development response to hypoxia mesoderm formation somitogenesis liver development heart looping osteoblast development immune system development activation of cysteine-type endopeptidase activity involved in apoptotic process immune response cell cycle arrest SMAD protein complex assembly endoderm development anatomical structure morphogenesis embryonic pattern specification positive regulation of epithelial to mesenchymal transition regulation of striated muscle tissue development regulation of transforming growth factor beta receptor signaling pathway cell differentiation negative regulation of cell growth BMP signaling pathway thyroid gland development positive regulation of chondrocyte differentiation regulation of transforming growth factor beta2 production negative regulation of osteoblast proliferation nodal signaling pathway T cell activation cell-cell junction organization positive regulation of nitric oxide biosynthetic process negative regulation of fat cell differentiation negative regulation of osteoblast differentiation negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter paraxial mesoderm morphogenesis developmental growth embryonic foregut morphogenesis embryonic cranial skeleton morphogenesis regulation of epithelial cell proliferation negative regulation of inflammatory response regulation of immune response regulation of binding negative regulation of cytosolic calcium ion concentration pericardium development SMAD protein signal transduction negative regulation of wound healing lens fiber cell differentiation extrinsic apoptotic signaling pathway positive regulation of extracellular matrix assembly positive regulation of pri-miRNA transcription from RNA polymerase II promoter MAPK cascade osteoblast differentiation release of cytochrome c from mitochondria regulation of transcription from RNA polymerase II promoter JNK cascade negative regulation of cell proliferation response to gamma radiation gene expression positive regulation of gene expression negative regulation of gene expression signal transduction involved in regulation of gene expression negative regulation of ossification adrenal gland development positive regulation of cell migration positive regulation of bone mineralization primary miRNA processing negative regulation of lipopolysaccharide-mediated signaling pathway developmental process positive regulation of interleukin-1 beta production positive regulation of transforming growth factor beta3 production activin receptor signaling pathway cellular response to platelet-derived growth factor stimulus wound healing negative regulation of protein catabolic process response to cocaine positive regulation of protein import into nucleus negative regulation of apoptotic process negative regulation of cell differentiation positive regulation of transcription, DNA-templated protein stabilization positive regulation of positive chemotaxis positive regulation of stress fiber assembly regulation of mitochondrial membrane potential positive regulation of focal adhesion assembly transdifferentiation positive regulation of SMAD protein import into nucleus regulation of dendritic spine morphogenesis trophoblast cell migration negative regulation of lung blood pressure cellular response to glucose stimulus cellular response to growth factor stimulus cellular response to transforming growth factor beta stimulus negative regulation of transforming growth factor beta production positive regulation of canonical Wnt signaling pathway apoptotic signaling pathway response to alcohol cellular response to virus transforming growth factor beta receptor superfamily signaling pathway regulation of miRNA transcription negative regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of cardiac muscle hypertrophy in response to stress response to angiotensin skeletal system development in utero embryonic development apoptotic process gastrulation cell proliferation positive regulation of alkaline phosphatase activity proteasome-mediated ubiquitin-dependent protein catabolic process response to transforming growth factor beta mesoderm development primitive hemopoiesis
          It has to be done as per old AB suggested Products section.

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