Hamburger Menu Button
Thermo Fisher Scientific Logo
登入
還沒有帳戶嗎 ? 立即註冊
  • 產品
    • Antibodies
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Cell Culture Media
    • Chemicals
    • Western Blot Products
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Greener Products
    • 查看所有產品類別
  • 應用
    • 生物製程
    • 細胞培養和轉染
    • 食品和飲料
    • 實驗室解決方案
    • 製藥和生物製藥
    • 即時定量PCR
    • 半導體分析
    • 臨床和診斷
    • 數位解決方案
    • 分子光譜、同位素與元素分析
    • 查看所有應用與技術
  • 服務
    • 360° CDMO 和 CRO 服務
    • CDMO 服务
    • 临床试验 CRO 服务
    • 客製化服務
    • 金融與租賃服務
    • 儀器服務
    • 實驗室資訊學
    • 授權與商業供應
    • 培訓服務
    • 查看所有服務
  • 支援
    • 線上訂購協助
    • 數位解決方案
    • 產品支持
    • 技術相關資訊
    • 培訓與教育
    • 查看所有協助與支援
  • 熱門商品
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • 我們的服務對象
    • 生物技術
    • 生物製藥
    • CDMO
    • 實驗室診斷
    • 工業與應用科學
  • 特價商品
  • 聯絡我們
  • 快速訂購
  • 訂單狀態與貨物追蹤
  • 文件和證書
Thermo Fisher Scientific Logo

Search

Search All
Search button
          • 訂單查詢
          • 快速訂購
          • 登入
            登入
            還沒有帳戶嗎 ? 立即註冊
            • 帳戶
            • 訂單
            • Connect: Lab, Data, Apps
            • 客製化產品與項目
            • 儀器管理
          • Primary Antibodies ›
          • SMAD3 Antibodies

          Rockland

          SMAD3 Polyclonal Antibody

          View all (70) SMAD3 antibodies

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support

          Cite SMAD3 Polyclonal Antibody

          Additional Information:
          {{banner.description}}
          {{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
          • Antibody Testing Data (2)
          SMAD3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          SMAD3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          SMAD3 Antibody (600-401-920) in IHC (P)

          Rocklands Affinity Purified anti-Smad3 antibody shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          SMAD3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SMAD3 Antibody in Western Blot (WB)
          SMAD3 Polyclonal Antibody

          Product Details

          600-401-920

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:500-1:3,000
          -

          ELISA (ELISA)

          1:10,000-1:40,000
          -
          Product Specifications

          Species Reactivity

          Bovine, Chicken, Human, Mouse, Pig, Rat, Xenopus, Zebrafish

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of human SMAD3 protein.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.2 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

          Contains

          0.01% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

          SMAD3 affinity-purified antibody is directed against human Smad3 protein. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat, swine, and chicken sources based on 100% homology for the immunogen sequence. ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated human SMAD3.

          Target Information

          SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

          Have you cited this product in a publication?
          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: DKFZP586N0721; DKFZp686J10186; JV15-2; MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 3; MAD homolog 3a; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; MAD-3; mad3; MGC60396; mMad3; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD; SMAD 3; SMAD family member 3; SMAD, mothers against DPP homolog 3; TGF-beta response effector Smad3; transcription factor; unnamed protein product

          View more View less

          Gene Aliases: AU022421; hMAD-3; hSMAD3; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; mad3; MADH3; madh3-A; madh3a; Smad 3; SMAD3; wu:fa99e03; XELAEV_18018454mg; XenMLP; Xmad3; XSmad3

          View more View less

          UniProt ID: (Pig) P84024, (Chicken) P84023, (Human) P84022, (Mouse) Q8BUN5, (Rat) P84025

          View more View less

          Entrez Gene ID: (Pig) 397260, (Chicken) 395132, (Bovine) 515125, (Human) 4088, (Mouse) 17127, (Rat) 25631, (Xenopus) 378633, (Zebrafish) 58092

          View more View less

          Function(s)
          core promoter proximal region sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding metal ion binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription cofactor activity transforming growth factor beta receptor binding collagen binding transcription factor binding zinc ion binding DEAD/H-box RNA helicase binding protein kinase binding phosphatase binding chromatin DNA binding ubiquitin protein ligase binding mineralocorticoid receptor binding glucocorticoid receptor binding protein homodimerization activity ubiquitin binding bHLH transcription factor binding co-SMAD binding I-SMAD binding R-SMAD binding primary miRNA binding transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding transcription corepressor binding DNA binding chromatin binding double-stranded DNA binding protein binding beta-catenin binding ligand-dependent nuclear receptor binding enzyme binding sterol response element binding identical protein binding sequence-specific DNA binding RNA polymerase II sequence-specific DNA binding transcription factor binding DNA-binding transcription factor binding promoter-specific chromatin binding transcription factor activity, RNA polymerase II core promoter sequence-specific transcription factor activity, protein binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity transcription regulatory region DNA binding SMAD binding protein heterodimerization activity DNA-binding transcription factor gene-specific transcriptional regulator
          Process(es)
          regulation of transcription, DNA-templated transforming growth factor beta receptor signaling pathway negative regulation of transcription from RNA polymerase II promoter ureteric bud development response to hypoxia mesoderm formation somitogenesis liver development heart looping osteoblast development immune system development activation of cysteine-type endopeptidase activity involved in apoptotic process immune response cell cycle arrest SMAD protein complex assembly endoderm development anatomical structure morphogenesis embryonic pattern specification positive regulation of epithelial to mesenchymal transition regulation of striated muscle tissue development regulation of transforming growth factor beta receptor signaling pathway cell differentiation negative regulation of cell growth BMP signaling pathway thyroid gland development positive regulation of chondrocyte differentiation regulation of transforming growth factor beta2 production negative regulation of osteoblast proliferation nodal signaling pathway T cell activation cell-cell junction organization positive regulation of nitric oxide biosynthetic process negative regulation of fat cell differentiation negative regulation of osteoblast differentiation negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter paraxial mesoderm morphogenesis developmental growth embryonic foregut morphogenesis embryonic cranial skeleton morphogenesis regulation of epithelial cell proliferation negative regulation of inflammatory response regulation of immune response regulation of binding negative regulation of cytosolic calcium ion concentration pericardium development SMAD protein signal transduction negative regulation of wound healing lens fiber cell differentiation extrinsic apoptotic signaling pathway positive regulation of extracellular matrix assembly positive regulation of pri-miRNA transcription from RNA polymerase II promoter MAPK cascade skeletal system development osteoblast differentiation in utero embryonic development release of cytochrome c from mitochondria regulation of transcription from RNA polymerase II promoter apoptotic process JNK cascade gastrulation cell proliferation negative regulation of cell proliferation positive regulation of gene expression negative regulation of gene expression signal transduction involved in regulation of gene expression negative regulation of ossification adrenal gland development positive regulation of cell migration positive regulation of bone mineralization primary miRNA processing developmental process positive regulation of interleukin-1 beta production positive regulation of transforming growth factor beta3 production activin receptor signaling pathway wound healing negative regulation of protein catabolic process positive regulation of protein import into nucleus negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of cell differentiation positive regulation of transcription, DNA-templated protein stabilization positive regulation of positive chemotaxis positive regulation of stress fiber assembly regulation of mitochondrial membrane potential positive regulation of focal adhesion assembly transdifferentiation positive regulation of SMAD protein import into nucleus trophoblast cell migration negative regulation of lung blood pressure cellular response to glucose stimulus cellular response to growth factor stimulus response to transforming growth factor beta cellular response to transforming growth factor beta stimulus positive regulation of canonical Wnt signaling pathway apoptotic signaling pathway cellular response to virus transforming growth factor beta receptor superfamily signaling pathway regulation of miRNA transcription negative regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of cardiac muscle hypertrophy in response to stress response to angiotensin negative regulation of protein phosphorylation transcription, DNA-templated transcription from RNA polymerase II promoter transport positive regulation of alkaline phosphatase activity evasion or tolerance of host defenses by virus positive regulation of catenin import into nucleus intracellular signal transduction positive regulation of transcription factor import into nucleus activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway mesoderm development primitive hemopoiesis
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          產品訂購 Plus Icon Minus Icon
          • 訂單狀態
          • 訂購協助
          • 快速訂購
          • 現貨供應中心
          • 電子採購
          支持 Plus Icon Minus Icon
          • 協助與支持
          • 聯絡我們
          • 技術支持中心
          • 搜尋文件和證書
          • 回報網站問題
          資源 Plus Icon Minus Icon
          • 學習中心
          • 促銷活動
          • 活動和網路研討會
          • 社群媒體
          關於 Thermo Fisher Plus Icon Minus Icon
          • 關於我們 關於我們
          • 人才招募 人才招募
          • 投資者資訊 投資者資訊
          • 新聞 新聞
          • 企業社會責任 企業社會責任
          • 商標
          • 政策和聲明
          品牌 Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Policy
          • Price & Freight Policy
          © 2006-2026 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          Taiwan flag icon
          Taiwan

          Your items have has been added!


          Host server : magellan-search-green-b49b87d85-pgj68:80/100.66.79.163:80.
          git-commit: 5b8c860b7cdb41e9cfe07630520f6b51e109d38e
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.47.0-Offline