Keep as concentrated solution.
Positive Control: HeLa, mouse brain, rat brain, SMAD3-transfected 293T.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
SMAD3 (hMAD-3; hSMAD3; HSPC193; JV15-2; MADH3; MGC60396) is located in gene map locus 15q22. 33 and is a member of SMAD family of proteins, is involved in TGF-beta signaling cascade. A human homolog of Drosophila Mad protein, SMAD3 consists of N-terminal DNA-binding MH1 domain, a linker region and a C-terminal MH2 domain. It is a receptor regulated SMAD (R-SMAD) that functions downstream of TGF-beta and activin receptors and mediates their signaling. It is recruited by SARA (Smad anchor for receptor activation) to the receptor kinase for phosphorylation. Upon phosphorylation, SMAD3 disassociates from SARA, forms a complex with SMAD4 and transmigrates into the nucleus where it complexes with other cofactors and acts as a transcription factor. It has an indispensable role in cell proliferation, differentiation, apoptosis and formation of ECM. Loss of SMAD3 results in Childhood T-cell leukemia.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DKFZp586N0721 antibody; DKFZp686J10186 antibody; hMAD-3; hMAD-3 antibody; hSMAD3; hSMAD3 antibody; HSPC193 antibody; HsT17436 antibody; JV15-2; JV15-2 antibody; MAD; MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 3; MAD homolog 3 antibody; mad homolog JV15-2; mad homolog JV15-2 antibody; mad protein homolog; mad protein homolog antibody; MAD, mothers against decapentaplegic homolog 3; mad3; mad3 antibody; MADH3 antibody; MGC60396 antibody; mMad3; Mothers against decapentaplegic homolog 3; mothers against decapentaplegic homolog 3 antibody; mothers against DPP homolog 3; mothers against DPP homolog 3 antibody; SMA- and MAD-related protein 3; SMA- and MAD-related protein 3 antibody; SMAD; SMAD 3; SMAD family member 3; SMAD family member 3 antibody; SMAD, mothers against DPP homolog 3; SMAD3 antibody
Gene Aliases: AU022421; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; mad3; MADH3; Smad 3; SMAD3
Molecular Function: transcription factor