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Immunofluorescent analysis of HeLa cells using a SMAD4 polyclonal antibody (Product # PA5-14845) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Actin filaments were stained with dye-conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 255-284 amino acids from human SMAD4|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse and rat based on sequence homology.
SMAD4 is the common SMAD (co-SMAD)mediator of signal transduction by TGF-beta (transforming growth factor). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; DPC4; hSMAD4; MAD homolog 4; MADH4; mothers against decapentaplegic, Drosophila, homolog of, 4; Mothers against DPP homolog 4; SMAD 4; SMAD, mothers against DPP homolog 4; SMAD4
DPC4; JIP; MADH4; MYHRS; SMAD4