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Product Details
PA5-28915
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 209 of Human SMAD5
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2546391
Product Specific Information
Recommended positive controls: HeLa, Raw264.7, C2C12, Rat2.^M
^M
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (94%), Chicken (98%), Rhesus Monkey (100%), Bovine (100%).^M
^M
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp781C1895; DKFZp781O1323; Dwarfin-C; Dwf-C; hSmad5; JV5-1; MAD homolog 5; MAD, mothers against decapentaplegic homolog 5; Mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; mothers against DPP homolog 5; mSmad5; OTTHUMP00000223331; SMA- and MAD-related protein 5; SMAD; SMAD 5; SMAD family member 5; SMAD, mothers against DPP homolog 5
Gene Aliases: 1110051M15Rik; AI451355; Dwf-C; DWFC; JV5-1; MADH5; Msmad5; MusMLP; SMAD5
UniProt ID: (Human) Q99717, (Mouse) P97454, (Rat) Q9R1V3
Entrez Gene ID: (Human) 4090, (Mouse) 17129, (Rat) 59328
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, sequence-specific DNA binding
receptor signaling protein activity
protein binding
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
ubiquitin protein ligase binding
metal ion binding
DNA binding
DNA-binding transcription factor
gene-specific transcriptional regulator
ureteric bud development
Mullerian duct regression
osteoblast fate commitment
transcription, DNA-templated
protein phosphorylation
signal transduction
transforming growth factor beta receptor signaling pathway
germ cell development
embryonic pattern specification
erythrocyte differentiation
BMP signaling pathway
positive regulation of osteoblast differentiation
positive regulation of transcription, DNA-templated
cartilage development
cardiac muscle contraction
bone development
SMAD protein signal transduction
cellular response to organic cyclic compound
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
angiogenesis
regulation of transcription, DNA-templated
multicellular organism development
cell differentiation
positive regulation of transcription from RNA polymerase II promoter
cellular response to BMP stimulus
ossification
regulation of ossification
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