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Invitrogen
SMAD6 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
SMAD6 Antibody (PA1-41026) in IHC (P)
Immunohistochemical analysis of SMAD6 in immersion fixed paraffin-embedded sections of human lung. Samples were incubated in SMAD6 polyclonal antibody (Product # PA1-41026) using a dilution of 5 µg/mL for 1 hour at room temperature followed by Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Nuclear staining was localized to the... View More
Product Details
PA1-41026
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Sheep,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide made to an internal portion of human SMAD6 (between amino acids 30-150) [UniProt O43541].
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2302345
Product Specific Information
This antibody is predicted to react with equine based on sequence homology.
Suggested positive control: HeLa or NIH-3T3 whole cell lysate.
Target Information
SMAD6 (mothers against decapentaplegic homolog 6) is an antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MAD homolog 6; Mad homolog 7; Mothers against decapentaplegic homolog 6; mothers against decapentaplegic, drosophila, homolog of, 6; mothers against DPP homolog 6; SMAD; SMAD 6; SMAD family member 6; SMAD SMAD; SMAD, mothers against DPP homolog 6
Gene Aliases: AOVD2; b2b390Clo; HsT17432; MADH6; MADH7; Msmad6; SMAD6
UniProt ID: (Human) O43541, (Mouse) O35182
Entrez Gene ID: (Human) 4091, (Sheep) 101103068, (Mouse) 17130, (Rat) 367100
RNA polymerase II core promoter proximal region sequence-specific DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity
ubiquitin protein ligase binding
type I transforming growth factor beta receptor binding
identical protein binding
transcription regulatory region DNA binding
metal ion binding
co-SMAD binding
I-SMAD binding
R-SMAD binding
type I activin receptor binding
DNA binding
molecular_function
DNA-binding transcription factor
gene-specific transcriptional regulator
ureteric bud development
outflow tract septum morphogenesis
mitral valve morphogenesis
pulmonary valve morphogenesis
ventricular septum development
transcription, DNA-templated
immune response
transforming growth factor beta receptor signaling pathway
zygotic specification of dorsal/ventral axis
negative regulation of cell proliferation
negative regulation of SMAD protein complex assembly
negative regulation of ossification
BMP signaling pathway
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of BMP signaling pathway
cell-substrate adhesion
response to laminar fluid shear stress
aorta development
negative regulation of apoptotic process
response to estrogen
fat cell differentiation
negative regulation of pathway-restricted SMAD protein phosphorylation
coronary vasculature development
regulation of transcription, DNA-templated
intracellular signal transduction
positive regulation of vasoconstriction
negative regulation of vasodilation
cardiac vascular smooth muscle cell development
coronary vasculature morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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