Invitrogen

SMAD6 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure

2 References

View all (9) SMAD6 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

SMAD6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of SMAD6 in immersion fixed paraffin-embedded sections of human lung. Samples were incubated in SMAD6 polyclonal antibody (Product # PA1-41026) using a dilution of 5 µg/mL for 1 hour at room temperature followed by Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Nuclear staining was localized to the pneumocytes.

SMAD6 Antibody in Western Blot (WB) — Western blot analysis of SMAD6 in 0.5 mg/mL HepG2 lysate. Samples were incubated in SMAD6 polyclonal (Product # PA1-41026). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

SMAD6 Antibody — Both, shRNAs or overexpression constructs were introduced by lentiviral transduction followed by selection with puromycin (P) or neomycin (N). Panel A and C represents Taqman-based qPCR data and panel B and D the corresponding protein expression data from the same treatment replicates. SMAD6 antibody from Life Technologies (PA1-41026), SMAD7 antibody from R&D systems (MAB2029).

Product Details

PA1-41026

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

5-10 µg/mL

Immunocytochemistry (ICC/IF)

1-5 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Sheep, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide made to an internal portion of human SMAD6 (between amino acids 30-150) [UniProt O43541].

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2302345

Product Specific Information

This antibody is predicted to react with equine based on sequence homology.

Suggested positive control: HeLa or NIH-3T3 whole cell lysate.

Target Information

SMAD6 (mothers against decapentaplegic homolog 6) is an antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The transcriptomic and epigenetic map of vascular quiescence in the continuous lung endothelium.

eLife

Schlereth K,Weichenhan D,Bauer T,Heumann T,Giannakouri E,Lipka D,Jaeger S,Schlesner M,Aloy P,Eils R,Plass C,Augustin HG

Published figure using SMAD6 polyclonal antibody (Product # PA1-41026) in Western Blot

Fri May 11 00:00:00 EDT 2018

Novel effects of rosiglitazone on SMAD2 and SMAD3 signaling in white adipose tissue of diabetic rats.

Obesity (Silver Spring, Md.)

Beaudoin MS,Snook LA,Arkell AM,Stefanson A,Wan Z,Simpson JA,Holloway GP,Wright DC

PA1-41026 was used in western blot to study the effect of rosiglitazone treatment on the transforming growth factor-beta/SMAD signaling pathway in white adipose tissue of diabetic rats

Tue Jul 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: MAD homolog 6; Mad homolog 7; Mothers against decapentaplegic homolog 6; mothers against decapentaplegic, drosophila, homolog of, 6; mothers against DPP homolog 6; SMAD; SMAD 6; SMAD family member 6; SMAD SMAD; SMAD, mothers against DPP homolog 6

Gene Aliases: AOVD2; b2b390Clo; HsT17432; MADH6; MADH7; Msmad6; SMAD6

UniProt ID: (Human) O43541, (Mouse) O35182

Entrez Gene ID: (Human) 4091, (Sheep) 101103068, (Mouse) 17130, (Rat) 367100

Molecular Function: DNA-binding transcription factor gene-specific transcriptional regulator