|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptides corresponding to residues R(12) L W R S R A P G G E D E E E G A G(29) and E(36) L R G E G A T D S R A H G A(50) of human SMAD7.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||4-6 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with chimpanzee, canine, pufferfish, xenopus and zebrafish based on sequence homology.
Suggested positive control: A549, HepG2.
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Novel effects of rosiglitazone on SMAD2 and SMAD3 signaling in white adipose tissue of diabetic rats.
PA1-41506 was used in western blot to study the effect of rosiglitazone treatment on the transforming growth factor-beta/SMAD signaling pathway in white adipose tissue of diabetic rats
|Beaudoin MS,Snook LA,Arkell AM,Stefanson A,Wan Z,Simpson JA,Holloway GP,Wright DC||Obesity (Silver Spring, Md.) (22:1632)||2014|
Smad7 regulates terminal maturation of chondrocytes in the growth plate.
PA1-41506 was used in immunohistochemistry to study the role of Smad7 in modulating chondrocyte terminal maturation at the growth plate using knock out mice
|Estrada KD,Wang W,Retting KN,Chien CT,Elkhoury FF,Heuchel R,Lyons KM||Developmental biology (382:375)||2013|