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SMAD7 Antibody (TA890026) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMAD7(RC210400, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-SMAD7 r. Positive lysates LY416991 (100 µg) and LC416991 (20 µg) can be purchased separately from OriGene.
Product Details
TA890026
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein of human SMAD7
Conjugate
Form
Liquid
Concentration
2.57 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Target Information
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ16482; hSMAD7; MAD (mothers against decapentaplegic, Drosophila) homolog 7; MAD homolog 7; MAD homolog 8; Mothers against decapentaplegic homolog 7; Mothers against decapentaplegic homolog 8; Mothers against decapentaplegic, drosophila, homolog of, 7; mothers against DPP homolog 8; SMAD; SMAD 7; SMAD family member 7; SMAD, mothers against DPP homolog 7
Gene Aliases: CRCS3; MADH7; MADH8; SMAD7
UniProt ID: (Human) O15105
Entrez Gene ID: (Human) 4092
transcription factor activity, sequence-specific DNA binding
protein binding
collagen binding
beta-catenin binding
transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity
ubiquitin protein ligase binding
type I transforming growth factor beta receptor binding
transcription regulatory region DNA binding
metal ion binding
activin binding
I-SMAD binding
DNA-binding transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
ureteric bud development
transcription, DNA-templated
transforming growth factor beta receptor signaling pathway
regulation of epithelial to mesenchymal transition
negative regulation of epithelial to mesenchymal transition
negative regulation of peptidyl-threonine phosphorylation
negative regulation of transcription by competitive promoter binding
regulation of transforming growth factor beta receptor signaling pathway
positive regulation of cell-cell adhesion
negative regulation of cell migration
BMP signaling pathway
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of BMP signaling pathway
negative regulation of protein ubiquitination
positive regulation of protein ubiquitination
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
regulation of activin receptor signaling pathway
negative regulation of peptidyl-serine phosphorylation
adherens junction assembly
response to laminar fluid shear stress
cellular protein complex localization
intracellular signal transduction
negative regulation of sequence-specific DNA binding transcription factor activity
positive regulation of transcription from RNA polymerase II promoter
artery morphogenesis
protein stabilization
negative regulation of ubiquitin-protein transferase activity
ventricular cardiac muscle tissue morphogenesis
regulation of cardiac muscle contraction
regulation of ventricular cardiac muscle cell membrane depolarization
pathway-restricted SMAD protein phosphorylation
negative regulation of pathway-restricted SMAD protein phosphorylation
ventricular septum morphogenesis
cellular response to transforming growth factor beta stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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