Proteintech

SMARCD1 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

SMARCD1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of SH-SY5Y cells, using SMARCD1 antibody 10998-2-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

SMARCD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lymphoma using 10998-2-AP (SMARCD1 antibody) at dilution of 1:50 (under 10x lens).

SMARCD1 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10998-2-AP (SMARCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

SMARCD1 Antibody in Immunoprecipitation (IP) — IP result of anti-SMARCD1 (IP:10998-2-AP, 3ug; Detection:10998-2-AP 1:600) with mouse brain tissue lysate 4000ug.

Product Details

10998-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SMARCD1 Fusion Protein Ag1453 (126-476 aa encoded by BC009368)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: RLDIQEALK RPIKQKRKLR IFISNTFNPA KSDAEDGEGT VASWELRVEG RLLEDSALSK YDATKQKRKF SSFFKSLVIE LDKDLYGPDN HLVEWHRTAT TQETDGFQVK RPGDVNVRCT VLLMLDYQPP QFKLDPRLAR LLGIHTQTRP VIIQALWQYI KTHKLQDPHE REFVICDKYL QQIFESQRMK FSEIPQRLHA LLMPPEPIII NHVISVDPND QKKTACYDID VEVDDTLKTQ MNSFLLSTAS QQEIATLDNK IHETIETINQ LKTQREFMLS FARDPQGFIN DWLQSQCRDL KTMTDVVGNP EEERRAEFYF QPWAQEAVCR YFYSKVQQRR QELEQALGIR NT (126-476 aa encoded by BC009368)

Target Information

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SMARCD1 regulates senescence-associated lipid accumulation in hepatocytes.

NPJ aging and mechanisms of disease

Inoue C,Zhao C,Tsuduki Y,Udono M,Wang L,Nomura M,Katakura Y

Tue Sep 29 00:00:00 EDT 2020

SMARCD1 regulates senescence-associated lipid accumulation in hepatocytes.

NPJ aging and mechanisms of disease

Inoue C,Zhao C,Tsuduki Y,Udono M,Wang L,Nomura M,Katakura Y

Tue Sep 29 00:00:00 EDT 2020

HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.

Nucleic acids research

Zhu X,Lan B,Yi X,He C,Dang L,Zhou X,Lu Y,Sun Y,Liu Z,Bai X,Zhang K,Li B,Li MJ,Chen Y,Zhang L

Thu Jul 09 00:00:00 EDT 2020

BAF60a deficiency uncouples chromatin accessibility and cold sensitivity from white fat browning.

Nature communications

Liu T,Mi L,Xiong J,Orchard P,Yu Q,Yu L,Zhao XY,Meng ZX,Parker SCJ,Lin JD,Li S

Wed May 13 00:00:00 EDT 2020

DLEU1 contributes to ovarian carcinoma tumourigenesis and development by interacting with miR-490-3p and altering CDK1 expression.

Journal of cellular and molecular medicine

Wang LL,Sun KX,Wu DD,Xiu YL,Chen X,Chen S,Zong ZH,Sang XB,Liu Y,Zhao Y

Wed Nov 01 00:00:00 EDT 2017

DLEU1 contributes to ovarian carcinoma tumourigenesis and development by interacting with miR-490-3p and altering CDK1 expression.

Journal of cellular and molecular medicine

Wang LL,Sun KX,Wu DD,Xiu YL,Chen X,Chen S,Zong ZH,Sang XB,Liu Y,Zhao Y

Wed Nov 01 00:00:00 EDT 2017

MicroRNA-490-3P targets CDK1 and inhibits ovarian epithelial carcinoma tumorigenesis and progression.

Cancer letters

Chen S,Chen X,Xiu YL,Sun KX,Zhao Y

Sun Jun 28 00:00:00 EDT 2015

Phosphorylation of Williams syndrome transcription factor by MAPK induces a switching between two distinct chromatin remodeling complexes.

The Journal of biological chemistry

Oya H,Yokoyama A,Yamaoka I,Fujiki R,Yonezawa M,Youn MY,Takada I,Kato S,Kitagawa H

Fri Nov 20 00:00:00 EST 2009

Phosphorylation of Williams syndrome transcription factor by MAPK induces a switching between two distinct chromatin remodeling complexes.

The Journal of biological chemistry

Oya H,Yokoyama A,Yamaoka I,Fujiki R,Yonezawa M,Youn MY,Takada I,Kato S,Kitagawa H

Fri Nov 20 00:00:00 EST 2009

Bioinformatics

Protein Aliases: 60 kDa BRG-1/Brm-associated factor subunit A; BAF60A; BRG1-associated factor 60A; chromatin remodeling complex BAF60A subunit; mammalian chromatin remodeling complex BRG1-associated factor 60A; Protein D15KZ1; SMARCD1; SWI/SNF complex 60 kDa subunit; SWI/SNF complex 60 kDa subunit A; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1; Swp73-like protein

Gene Aliases: AA407987; BAF60A; CRACD1; D15Kz1; Rsc6p; SMARCD1

UniProt ID: (Human) Q96GM5, (Mouse) Q61466

Entrez Gene ID: (Human) 6602, (Mouse) 83797, (Rat) 363002

Molecular Function: chromatin/chromatin-binding, or -regulatory protein