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Invitrogen
SMC1 Polyclonal Antibody
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FIGURE: 1 / 6
SMC1 Antibody (PA5-29122) in ICC/IF
Immunofluorescence analysis of SMC1 was performed using MOLT-4 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SMC1 Rabbit Polyclonal Antibody (Product # PA5-29122) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2000 ... View More
Product Details
PA5-29122
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human SMC1A. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
Predicted reactivity: Mouse (99%), Rat (98%), Zebrafish (84%), Xenopus laevis (91%), Chicken (94%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromo proteins are conserved in eukaryotes and lead to mitotic chromosome segregatior defects. This suggests a critical function of SMC family proteins in mitotic chromosome dynamics. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. Specifically this SMC1/SMC3 complex is responsible for sister chromatid cohesion during metaphase. Mutations in the gene can lead to Cornelia de Lange syndrome 2.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp686L19178; epididymis secretory sperm binding protein; KIAA0178; MGC138332; mitosis-specific chromosome segregation protein like protein beta; mitosis-specific chromosome segregation protein SMC1 homolog; RP6-29D12.1; Sb1.8; segregation of mitotic chromosomes 1; SM1A; SMC protein 1A; SMC protein 1B; SMC-1-alpha; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC1 structural maintenance of chromosomes 1-like 2; SMC1alpha protein; Structural maintenance of chromosomes protein 1A; Structural maintenance of chromosomes protein 1B; unnamed protein product
Gene Aliases: CDLS2; DEE85; DXS423E; EIEE85; KIAA0178; SB1.8; SMC1; SMC1A; SMC1alpha; SMC1B; SMC1BETA; SMC1L1; SMC1L2; SMCB
UniProt ID: (Human) Q14683, (Human) Q8NDV3
Entrez Gene ID: (Human) 8243, (Human) 27127
sister chromatid cohesion
chromosome organization
meiotic cell cycle
mitotic sister chromatid segregation
DNA repair
cellular response to DNA damage stimulus
mitotic sister chromatid cohesion
response to radiation
establishment of mitotic sister chromatid cohesion
establishment of meiotic sister chromatid cohesion
somatic stem cell population maintenance
cell division
response to DNA damage checkpoint signaling
mitotic spindle assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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