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Invitrogen
SMC3 Polyclonal Antibody
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FIGURE: 1 / 13
SMC3 Antibody (PA5-29131) in ICC/IF
Immunofluorescence analysis of SMC3 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SMC3 Polyclonal Antibody (Product # PA5-29131) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More
Product Details
PA5-29131
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human SMC3. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HepG2, NIH-3T3, JC, BCL-1, PC-12.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (95%), Xenopus laevis (98%), Chicken (99%), Bovine (91%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Bamacan; basement membrane chondroitin sulfate proteoglycan; Basement membrane-associated chondroitin proteoglycan; Chondroitin sulfate proteoglycan 6; chondroitin sulfate proteoglycan 6 (bamacan); Chromosome segregation protein SmcD; Chromosome-associated polypeptide; hCAP; Mad member-interacting protein 1; SMC family; SMC protein 3; structural maintenace of chromosomes 3; Structural maintenance of chromosomes protein 3; unnamed protein product
Gene Aliases: BAM; Bamacan; BMH; CDLS3; CSPG6; HCAP; Mmip1; SMC-3; SMC3; SMC3L1; SmcD
UniProt ID: (Human) Q9UQE7, (Mouse) Q9CW03
Entrez Gene ID: (Human) 9126, (Mouse) 13006, (Rat) 29486
nucleotide binding
core promoter proximal region sequence-specific DNA binding
chromatin binding
double-stranded DNA binding
microtubule motor activity
protein binding
ATP binding
ATPase activity
mediator complex binding
protein heterodimerization activity
beta-tubulin binding
dynein complex binding
dynein binding
molecular_function
regulation of DNA replication
DNA repair
cellular response to DNA damage stimulus
cell cycle
sister chromatid cohesion
mitotic sister chromatid cohesion
mitotic nuclear division
meiotic nuclear division
signal transduction
stem cell population maintenance
negative regulation of DNA endoreduplication
chromosome organization
cell division
meiotic cell cycle
mitotic cell cycle
establishment of mitotic sister chromatid cohesion
establishment of meiotic sister chromatid cohesion
mitotic spindle assembly
mitotic spindle organization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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