|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 909 and 1199 of Human SMC3|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-29131 targets SMC3 in IF, IHC (P), and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-29131 immunogen is recombinant fragment corresponding to a region within amino acids 909 and 1199 of Human SMC3.
This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Identification of SUMO-2/3-modified proteins associated with mitotic chromosomes.
PA5-29131 was used in western blot to identify targets of SUMO2/3 during mitosis
|Cubeñas-Potts C,Srikumar T,Lee C,Osula O,Subramonian D,Zhang XD,Cotter RJ,Raught B,Matunis MJ||Proteomics (15:763)||2015|