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Invitrogen
SMPD4 Polyclonal Antibody
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FIGURE: 1 / 1
SMPD4 Antibody (PA5-143768) in ICC/IF
Immunocytochemistry analysis of SMPD4 in aldehyde-fixed and NP-40-permeabilized mouse C2C12 cells. The cells were labeled with rabbit SMPD4 polyclonal antibody (Product # PA5-143768). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
Product Details
PA5-143768
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
nSMase3 synthetic peptide corresponds to amino acids in the C-terminal region of mouse nSMase3. This sequence is highly conserved in rat and human nSMase3, and has low homology to other nSMase family members.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody detects an 85-90 kDa* band corresponding to nSMase3 on SDS-PAGE immunoblots of adult mouse heart and mouse C2C12 cells. The antibody works for western blot, ELISA, immunoprecipitation, and immunocytochemistry.
Target Information
The protein encoded by SMPD4 is a sphingomyelinase that catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. This gene is activated by DNA damage, cellular stress, and tumor necrosis factor, but it is downregulated by wild-type p53. The encoded protein localizes to the endoplasmic reticulum and Golgi network.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: neutral membrane (neutral sphingomyelinase-3); Neutral sphingomyelinase 3; Neutral sphingomyelinase III; neutral sphingomyelinase-3; nSMase-3; nSMase3; SKNY protein; Sphingomyelin phosphodiesterase 4; sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3)
Gene Aliases: 4122402O22Rik; KIAA1418; mKIAA1418; NET13; NSMASE-3; NSMASE3; RGD1310674; SKNY; SMPD4
UniProt ID: (Human) Q9NXE4, (Mouse) Q6ZPR5
Entrez Gene ID: (Human) 55627, (Mouse) 77626, (Rat) 303790
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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