Proteintech

SMYD2 Polyclonal Antibody

View all (14) SMYD2 antibodies

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Questions & Answers

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SMYD2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using SMYD2 antibody 21290-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

SMYD2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using SMYD2 antibody 21290-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

SMYD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 21290-1-AP (SMYD2 antibody) at dilution of 1:100 (under 40x lens).

SMYD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 21290-1-AP (SMYD2 antibody) at dilution of 1:100 (under 10x lens).

SMYD2 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — Mouse heart tissue were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Product Details

21290-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SMYD2 Fusion Protein Ag15823 (1-272 aa encoded by BC098305) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.21 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MRAEGLGGL ERFCSPGKGR GLRALQPFQV GDLLFSCPAY AYVLTVNERG NHCEYCFTRK EGLSKCGRCK QAFYCNVECQ KEDWPMHKLE CSPMVVFGEN WNPSETVRLT ARILAKQKIH PERTPSEKLL AVKEFESHLD KLDNEKKDLI QSDIAALHHF YSKHLGFPDN DSLVVLFAQV NCNGFTIEDE ELSHLGSAIF PDVALMNHSC CPNVIVTYKG TLAEVRAVQE IKPGEEVFTS YIDLLYPTED RNDRLRDSYF FTCECQECTT KDK (1-272 aa encoded by BC098305)

Target Information

SET domain-containing proteins, such as SMYD2, catalyze lysine methylation..

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Positive Expression of SMYD2 is Associated with Poor Prognosis in Patients with Primary Hepatocellular Carcinoma.

Journal of Cancer

Zuo SR,Zuo XC,He Y,Fang WJ,Wang CJ,Zou H,Chen P,Huang LF,Huang LH,Xiang H,Liu SK

Fri Apr 08 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: Histone methyltransferase SMYD2; HSKM-B; Lysine N-methyltransferase 3C; N-lysine methyltransferase SMYD2; SET and MYND domain-containing protein 2; zinc finger, MYND domain containing 14

Gene Aliases: 1110020E07Rik; 4930402C15; HSKM-B; KMT3C; SMYD2; ZMYND14

UniProt ID: (Human) Q9NRG4, (Mouse) Q8R5A0

Entrez Gene ID: (Human) 56950, (Mouse) 226830

Molecular Function: histone modifying enzyme

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated heart development negative regulation of cell proliferation histone H3-K36 methylation peptidyl-lysine monomethylation peptidyl-lysine dimethylation regulation of DNA damage response, signal transduction by p53 class mediator regulation of signal transduction by p53 class mediator regulation of transcription, DNA-templated chromatin modification histone methylation methylation

Process(es)

RNA polymerase II core binding p53 binding protein binding lysine N-methyltransferase activity protein-lysine N-methyltransferase activity histone-lysine N-methyltransferase activity metal ion binding histone methyltransferase activity (H3-K36 specific) rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity