Proteintech

SMYD2 Polyclonal Antibody

View all (13) SMYD2 antibodies

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SMYD2 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — Mouse heart tissue were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 21290-1-AP (SMYD2 antibody) at dilution of 1:100 (under 40x lens).

SMYD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 21290-1-AP (SMYD2 antibody) at dilution of 1:100 (under 10x lens).

SMYD2 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Hela cells, using SMYD2 antibody 21290-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

SMYD2 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Hela cells, using SMYD2 antibody 21290-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC)

1:10-1:100

-

Immunofluorescence (IF)

1:10-1:100

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Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SMYD2 Fusion Protein Ag15823

Conjugate

Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

SET domain-containing proteins, such as SMYD2, catalyze lysine methylation..

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Histone methyltransferase SMYD2; HSKM-B; Lysine N-methyltransferase 3C; N-lysine methyltransferase SMYD2; SET and MYND domain-containing protein 2; zinc finger, MYND domain containing 14

Gene Aliases: 1110020E07Rik; 4930402C15; HSKM-B; KMT3C; SMYD2; ZMYND14

UniProt ID: (Human) Q9NRG4, (Mouse) Q8R5A0

Entrez Gene ID: (Human) 56950, (Mouse) 226830

Molecular Function: transcription cofactor transcription factor

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated heart development negative regulation of cell proliferation histone H3-K36 methylation peptidyl-lysine monomethylation peptidyl-lysine dimethylation regulation of DNA damage response, signal transduction by p53 class mediator regulation of signal transduction by p53 class mediator regulation of transcription, DNA-templated chromatin modification histone methylation methylation

Process(es)

RNA polymerase II core binding p53 binding protein binding lysine N-methyltransferase activity protein-lysine N-methyltransferase activity histone-lysine N-methyltransferase activity metal ion binding histone methyltransferase activity (H3-K36 specific) rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity