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SMYD2 Polyclonal Antibody

View all (15) SMYD2 antibodies

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SMYD2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using SMYD2 antibody 21290-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

SMYD2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using SMYD2 antibody 21290-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

SMYD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 21290-1-AP (SMYD2 antibody) at dilution of 1:100 (under 40x lens).

SMYD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 21290-1-AP (SMYD2 antibody) at dilution of 1:100 (under 10x lens).

SMYD2 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — Mouse heart tissue were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SMYD2 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Product Details

21290-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

-

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SMYD2 Fusion Protein Ag15823 (1-272 aa encoded by BC098305)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MRAEGLGGL ERFCSPGKGR GLRALQPFQV GDLLFSCPAY AYVLTVNERG NHCEYCFTRK EGLSKCGRCK QAFYCNVECQ KEDWPMHKLE CSPMVVFGEN WNPSETVRLT ARILAKQKIH PERTPSEKLL AVKEFESHLD KLDNEKKDLI QSDIAALHHF YSKHLGFPDN DSLVVLFAQV NCNGFTIEDE ELSHLGSAIF PDVALMNHSC CPNVIVTYKG TLAEVRAVQE IKPGEEVFTS YIDLLYPTED RNDRLRDSYF FTCECQECTT KDK (1-272 aa encoded by BC098305)

Target Information

SET domain-containing proteins, such as SMYD2, catalyze lysine methylation..

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting SMYD2 inhibits prostate cancer cell growth by regulating c-Myc signaling.

Molecular carcinogenesis

Li J,Wan F,Zhang J,Zheng S,Yang Y,Hong Z,Dai B

Sat Jul 01 00:00:00 EDT 2023

Targeting SMYD2 inhibits prostate cancer cell growth by regulating c-Myc signaling.

Molecular carcinogenesis

Li J,Wan F,Zhang J,Zheng S,Yang Y,Hong Z,Dai B

Sat Jul 01 00:00:00 EDT 2023

Targeting SMYD2 inhibits prostate cancer cell growth by regulating c-Myc signaling.

Molecular carcinogenesis

Li J,Wan F,Zhang J,Zheng S,Yang Y,Hong Z,Dai B

Sat Jul 01 00:00:00 EDT 2023

Targeting SMYD2 inhibits prostate cancer cell growth by regulating c-Myc signaling.

Molecular carcinogenesis

Li J,Wan F,Zhang J,Zheng S,Yang Y,Hong Z,Dai B

Sat Jul 01 00:00:00 EDT 2023

Histone methyltransferase Smyd2 drives vascular aging by its enhancer-dependent activity.

Aging

Su Z,Su H,Xu J,Wei G,Qu L,Ni T,Yang D,Zhu Y

Wed Dec 28 00:00:00 EST 2022

Histone methyltransferase Smyd2 drives vascular aging by its enhancer-dependent activity.

Aging

Su Z,Su H,Xu J,Wei G,Qu L,Ni T,Yang D,Zhu Y

Wed Dec 28 00:00:00 EST 2022

Histone methyltransferase Smyd2 drives vascular aging by its enhancer-dependent activity.

Aging

Su Z,Su H,Xu J,Wei G,Qu L,Ni T,Yang D,Zhu Y

Wed Dec 28 00:00:00 EST 2022

Histone methyltransferase Smyd2 drives vascular aging by its enhancer-dependent activity.

Aging

Su Z,Su H,Xu J,Wei G,Qu L,Ni T,Yang D,Zhu Y

Wed Dec 28 00:00:00 EST 2022

Histone methyltransferase Smyd2 drives vascular aging by its enhancer-dependent activity.

Aging

Su Z,Su H,Xu J,Wei G,Qu L,Ni T,Yang D,Zhu Y

Wed Dec 28 00:00:00 EST 2022

Histone methyltransferase Smyd2 drives vascular aging by its enhancer-dependent activity.

Aging

Su Z,Su H,Xu J,Wei G,Qu L,Ni T,Yang D,Zhu Y

Wed Dec 28 00:00:00 EST 2022

Histone methyltransferase Smyd2 drives adipogenesis via regulating STAT3 phosphorylation.

Cell death & disease

Su H,Meng C,Xu J,Su Z,Xiao C,Yang D

Fri Oct 21 00:00:00 EDT 2022

STUB1-SMYD2 Axis Regulates Drug Resistance in Glioma cells.

Journal of molecular neuroscience : MN

Pan K,Hu B,Wang L,Yuan J,Xu W

Thu Sep 01 00:00:00 EDT 2022

STUB1-SMYD2 Axis Regulates Drug Resistance in Glioma cells.

Journal of molecular neuroscience : MN

Pan K,Hu B,Wang L,Yuan J,Xu W

Thu Sep 01 00:00:00 EDT 2022

Oleocanthal Attenuates Metastatic Castration-Resistant Prostate Cancer Progression and Recurrence by Targeting SMYD2.

Cancers

Siddique AB,Ebrahim HY,Tajmim A,King JA,Abdelwahed KS,Abd Elmageed ZY,El Sayed KA

Thu Jul 21 00:00:00 EDT 2022

Positive Expression of SMYD2 is Associated with Poor Prognosis in Patients with Primary Hepatocellular Carcinoma.

Journal of Cancer

Zuo SR,Zuo XC,He Y,Fang WJ,Wang CJ,Zou H,Chen P,Huang LF,Huang LH,Xiang H,Liu SK

Fri Apr 08 00:00:00 EDT 2022

Positive Expression of SMYD2 is Associated with Poor Prognosis in Patients with Primary Hepatocellular Carcinoma.

Journal of Cancer

Zuo SR,Zuo XC,He Y,Fang WJ,Wang CJ,Zou H,Chen P,Huang LF,Huang LH,Xiang H,Liu SK

Fri Apr 08 00:00:00 EDT 2022

SMYD2 targets RIPK1 and restricts TNF-induced apoptosis and necroptosis to support colon tumor growth.

Cell death & disease

Yu YQ,Thonn V,Patankar JV,Thoma OM,Waldner M,Zielinska M,Bao LL,Gonzalez-Acera M,Wallmüller S,Engel FB,Stürzl M,Neurath MF,Liebing E,Becker C

Wed Jan 12 00:00:00 EST 2022

SMYD2 suppresses p53 activity to promote glucose metabolism in cervical cancer.

Experimental cell research

Wang Y,Jin G,Guo Y,Cao Y,Niu S,Fan X,Zhang J

Thu Jul 15 00:00:00 EDT 2021

SMYD2 suppresses APC2 expression to activate the Wnt/β-catenin pathway and promotes epithelial-mesenchymal transition in colorectal cancer.

American journal of cancer research

Meng F,Liu X,Lin C,Xu L,Liu J,Zhang P,Zhang X,Song J,Yan Y,Ren Z,Zhang Y

Mon Sep 28 00:00:00 EDT 2020

SMYD2 suppresses APC2 expression to activate the Wnt/β-catenin pathway and promotes epithelial-mesenchymal transition in colorectal cancer.

American journal of cancer research

Meng F,Liu X,Lin C,Xu L,Liu J,Zhang P,Zhang X,Song J,Yan Y,Ren Z,Zhang Y

Mon Sep 28 00:00:00 EDT 2020

Inhibition of SMYD2 suppresses tumor progression by down-regulating microRNA-125b and attenuates multi-drug resistance in renal cell carcinoma.

Theranostics

Yan L,Ding B,Liu H,Zhang Y,Zeng J,Hu J,Yao W,Yu G,An R,Chen Z,Ye Z,Xing J,Xiao K,Wu L,Xu H

Mon Sep 14 00:00:00 EDT 2020

Regulation of EZH2 by SMYD2-Mediated Lysine Methylation Is Implicated in Tumorigenesis.

Cell reports

Zeng Y,Qiu R,Yang Y,Gao T,Zheng Y,Huang W,Gao J,Zhang K,Liu R,Wang S,Hou Y,Yu W,Leng S,Feng D,Liu W,Zhang X,Wang Y

Tue Nov 05 00:00:00 EST 2019

SMYD2 Drives Mesendodermal Differentiation of Human Embryonic Stem Cells Through Mediating the Transcriptional Activation of Key Mesendodermal Genes.

Stem cells (Dayton, Ohio)

Bai HJ,Zhang P,Ma L,Liang H,Wei G,Yang HT

Fri Nov 01 00:00:00 EDT 2019

Proteomic analysis of seminal extracellular vesicle proteins involved in asthenozoospermia by iTRAQ.

Molecular reproduction and development

Lin Y,Liang A,He Y,Li Z,Li Z,Wang G,Sun F

Sun Sep 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: Histone methyltransferase SMYD2; HSKM-B; Lysine N-methyltransferase 3C; N-lysine methyltransferase SMYD2; SET and MYND domain-containing protein 2; zinc finger, MYND domain containing 14

Gene Aliases: 1110020E07Rik; 4930402C15; HSKM-B; KMT3C; SMYD2; ZMYND14

UniProt ID: (Human) Q9NRG4, (Mouse) Q8R5A0

Entrez Gene ID: (Human) 56950, (Mouse) 226830

Molecular Function: histone modifying enzyme

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated heart development negative regulation of cell proliferation histone H3-K36 methylation peptidyl-lysine monomethylation peptidyl-lysine dimethylation regulation of DNA damage response, signal transduction by p53 class mediator regulation of signal transduction by p53 class mediator regulation of transcription, DNA-templated chromatin modification histone methylation methylation

Process(es)

RNA polymerase II core binding p53 binding protein binding lysine N-methyltransferase activity protein-lysine N-methyltransferase activity histone-lysine N-methyltransferase activity metal ion binding histone methyltransferase activity (H3-K36 specific) rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity