Invitrogen

SMYD2 Polyclonal Antibody

2 Published Figures

View all (9) SMYD2 antibodies

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SMYD2 Antibody — Figure 1 Analysis of SMYD2 expression in patients with PTC. ( A, B ) IHC staining of SMYD2 in adjacent thyroid tissues ( A ) and PTC tissues ( B ). ( C ) Correlation between relative protein level of SMYD2 and different TNM stages. ( D ) RNA levels of SMYD2 were examined in PTC tissues and adjacent thyroid tissues. * P<0.05 by t test. Magnification: 400x.

SMYD2 Antibody — Published figure using SMYD2 polyclonal antibody (Product # PA5-51339)

SMYD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of SMYD2 in paraffin-embedded Human liver cancer tissue. Samples were probed using a SMYD2 polyclonal antibody (Product # PA5-51339) at a dilution of 1/35.

SMYD2 Antibody in Western Blot (WB) — Western blot analysis of Smyd2 was performed by loading Mouse heart tissue lysates (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a Smyd2 antibody (Product # PA5-51339) at a 1/350 dilution for 2 minutes, followed by Goat anti rabbit IgG at 1/8000 dilution.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

Western Blot (WB)

1:200-1:1000

Published Applications

Immunohistochemistry (IHC)

See 2 publications below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide of human SMYD2

Conjugate

Unconjugated

Form

Liquid

Concentration

2.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 40% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

RRID

AB_2636786

Product Specific Information

The antibody detects endogenous levels of total SMYD2 protein.

Target Information

SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Histone methyltransferase SMYD2; HSKM-B; KMT3C; Lysine N-methyltransferase 3C; N-lysine methyltransferase SMYD2; SET and MYND domain-containing protein 2; zinc finger, MYND domain containing 14

Gene Aliases: 1110020E07Rik; 4930402C15; HSKM-B; KMT3C; SMYD2; ZMYND14

UniProt ID: (Human) Q9NRG4, (Rat) Q7M6Z3, (Mouse) Q8R5A0

Entrez Gene ID: (Human) 56950, (Rat) 289372, (Mouse) 226830

Molecular Function: transcription cofactor transcription factor

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated heart development negative regulation of cell proliferation histone H3-K36 methylation peptidyl-lysine monomethylation peptidyl-lysine dimethylation regulation of DNA damage response, signal transduction by p53 class mediator regulation of signal transduction by p53 class mediator histone methylation regulation of transcription, DNA-templated chromatin modification methylation

Process(es)

RNA polymerase II core binding p53 binding protein binding lysine N-methyltransferase activity protein-lysine N-methyltransferase activity histone-lysine N-methyltransferase activity metal ion binding histone methyltransferase activity (H3-K36 specific) molecular_function rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity