Invitrogen

SMYD2 Polyclonal Antibody

3 Published Figures

View all (14) SMYD2 antibodies

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SMYD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of SMYD2 in paraffin-embedded Human liver cancer tissue. Samples were probed using a SMYD2 polyclonal antibody (Product # PA5-51339) at a dilution of 1/35.

SMYD2 Antibody in Western Blot (WB) — Western blot analysis of Smyd2 was performed by loading Mouse heart tissue lysates (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a Smyd2 antibody (Product # PA5-51339) at a 1/350 dilution for 2 minutes, followed by Goat anti rabbit IgG at 1/8000 dilution.

SMYD2 Antibody in Immunohistochemistry (IHC) — Figure 1 Protein expression of clear-cell (CCC)-associated biomarkers (A) , endometrioid (EC)-associated biomarkers (B) , and mucinous ovarian carcinoma (MC)-associated biomarkers (C) . Representative immunohistochemical staining intensities showing protein expression (negative vs. positive) in ovarian tumor cells for histotype-associated biomarkers (400 x magnification).

SMYD2 Antibody in Immunohistochemistry (IHC) — Figure 1 Analysis of SMYD2 expression in patients with PTC. ( A, B ) IHC staining of SMYD2 in adjacent thyroid tissues ( A ) and PTC tissues ( B ). ( C ) Correlation between relative protein level of SMYD2 and different TNM stages. ( D ) RNA levels of SMYD2 were examined in PTC tissues and adjacent thyroid tissues. * P<0.05 by t test. Magnification: 400x.

SMYD2 Antibody in Immunohistochemistry (IHC) — Published figure using SMYD2 polyclonal antibody (Product # PA5-51339) in Immunohistochemistry

Product Details

PA5-51339

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200-1:1,000	-
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide of human SMYD2
Conjugate	Unconjugated
Form	Liquid
Concentration	2.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 40% glycerol
Contains	0.05% sodium azide
Storage conditions	-20°C
RRID	AB_2636786

Product Specific Information

The antibody detects endogenous levels of total SMYD2 protein.

Target Information

SET domain-containing proteins, such as SMYD2, catalyze lysine methylation..

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Validation of Novel Prognostic Biomarkers for Early-Stage Clear-Cell, Endometrioid and Mucinous Ovarian Carcinomas Using Immunohistochemistry.

Frontiers in oncology

Engqvist H,Parris TZ,Kovács A,Rönnerman EW,Sundfeldt K,Karlsson P,Helou K

Published figure using SMYD2 polyclonal antibody (Product # PA5-51339) in Immunohistochemistry

Mon Sep 28 00:00:00 EDT 2020

SMYD2 promoter DNA methylation is associated with abdominal aortic aneurysm (AAA) and SMYD2 expression in vascular smooth muscle cells.

Clinical epigenetics

Toghill BJ,Saratzis A,Freeman PJ,Sylvius N,Bown MJ

Published figure using SMYD2 polyclonal antibody (Product # PA5-51339) in Immunohistochemistry

Thu Feb 28 00:00:00 EST 2019

Overexpression of SET and MYND Domain-Containing Protein 2 (SMYD2) Is Associated with Tumor Progression and Poor Prognosis in Patients with Papillary Thyroid Carcinoma.

Medical science monitor : international medical journal of experimental and clinical research

Xu W,Chen F,Fei X,Yang X,Lu X

Published figure using SMYD2 polyclonal antibody (Product # PA5-51339) in Immunohistochemistry

Mon Oct 15 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Histone methyltransferase SMYD2; HSKM-B; Lysine N-methyltransferase 3C; N-lysine methyltransferase SMYD2; SET and MYND domain-containing protein 2; zinc finger, MYND domain containing 14

Gene Aliases: 1110020E07Rik; 4930402C15; HSKM-B; KMT3C; SMYD2; ZMYND14

UniProt ID: (Human) Q9NRG4, (Rat) Q7M6Z3, (Mouse) Q8R5A0

Entrez Gene ID: (Human) 56950, (Rat) 289372, (Mouse) 226830

Molecular Function: histone modifying enzyme

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated heart development negative regulation of cell proliferation histone H3-K36 methylation peptidyl-lysine monomethylation peptidyl-lysine dimethylation regulation of DNA damage response, signal transduction by p53 class mediator regulation of signal transduction by p53 class mediator histone methylation regulation of transcription, DNA-templated chromatin modification methylation

Process(es)

RNA polymerase II core binding p53 binding protein binding lysine N-methyltransferase activity protein-lysine N-methyltransferase activity histone-lysine N-methyltransferase activity metal ion binding histone methyltransferase activity (H3-K36 specific) molecular_function rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity