Invitrogen
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:3,000 | View 1 publication 1 publication |
Immunocytochemistry (ICC/IF) |
1:100-1:1,000 | - |
Immunoprecipitation (IP) |
1:100-1:500 | - |
ChIP assay (ChIP) |
Assay-dependent | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse |
Published species |
Not Applicable |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant protein fragment corresponding to a region within amino acids 22 and 411 of Human SMYD3 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1.23 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7, with 20% glycerol |
Contains |
0.025% ProClin 300 |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2549392 |
Recommended positive controls: DDDDK-tagged SMYD3-transfected 293T, 293T, A431, HeLa, HepG2, A549, SKOV3, HCT116.
Predicted reactivity: Human (99%), Mouse (93%), Rat (94%), Pig (95%), Rhesus Monkey (99%), Bovine (83%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
SMYD3 (histone-lysine N-methyltransferase) methylates Lys-4 of histone H3 and Lys-5 of histone H4. It induces di- and tri-methylation. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bA74P14.1 (novel protein); Histone-lysine N-methyltransferase SMYD3; SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing), 1; zinc finger, MYND domain containing 1
Gene Aliases: 2410008A19Rik; bA74P14.1; KMT3E; SMYD3; ZMYND1; ZNFN3A1
UniProt ID: (Human) Q9H7B4, (Mouse) Q9CWR2
Entrez Gene ID: (Human) 64754, (Mouse) 69726
Molecular Function:
histone modifying enzyme
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