Invitrogen

SMYD3 Polyclonal Antibody

2 Published Figures

1 Reference

View all (14) SMYD3 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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SMYD3 Antibody in Western Blot (WB) — Western Blot analysis of SMYD3 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a SMYD3 Polyclonal Antibody (Product # PA5-31919) at a dilution of 1:1,000.

SMYD3 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of SMYD3 showing staining in the cytoplasm of HCT116 cells. HCT116 cells were fixed in ice-cold MeOH for 5 min and stained using a SMYD3 polyclonal antibody (Product # PA5-31919) diluted at 1:500. Blue: Hoechst 33342 staining.

SMYD3 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of SMYD3 was performed in 293T whole cell extracts using 5 µg of SMYD3 Polyclonal Antibody (Product # PA5-31919). Samples were transferred to a membrane and probed with SMYD3 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.

SMYD3 Antibody in ChIP assay (ChIP) — Cross-linked ChIP analysis of SMYD3 in HepG2 chromatin extract using 5 µg of either normal rabbit IgG or anti-SMYD3 antibody (Product # PA5-31919). The precipitated DNA was detected by PCR with primer set targeting to 5' flanking region of Nkx2.8.

SMYD3 Antibody — Fig 5 Gene ablation techniques show no dependence on SMYD2 or SMYD3 for cancer cell proliferation. Waterfall plot representing LogP RSA scores for sgRNAs targeting A) SMYD2 and B) SMYD3. 313 cell lines were infected with a library of 6500 sgRNAs targeting 600 different genes. LogP RSA scores represent depletion of guides from an infected cell population. Each bar represents a different cell line. Bars are colored by cancer subtype. C) Percent confluency of Hep3B cells infected with CRISPR viruses containing CAS9 and sgRNAs targeting HBE-1, EZH2 (negative controls) or SMYD3. Cell density was evaluated using an Incucyte Zoom. Growth curves were initiated 24 days following virus infection and puromycin selection. Plotted data is the average of three biological replicates. Error bars represent standard deviation (not readily visible on scale). D) SMYD3 western blot of lysates derived from Hep3B cells infected with CAS9 and SMYD3 sgRNA. Parental Hep3Bs and Hep3Bs stably infected with HBE-1, EZH2 (negative controls) or SMYD3 were lysed and probed for SMYD3 levels by western. GAPDH levels were evaluated as a loading control.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3000

View 1 publication 1 publication

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunoprecipitation (IP)

1:100-1:500

ChIP assay (ChIP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 22 and 411 of Human SMYD3

Conjugate

Unconjugated

Form

Liquid

Concentration

1.32 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2549392

Product Specific Information

Recommended positive controls: DDDDK-tagged SMYD3-transfected 293T, 293T, A431, HeLa, HepG2, A549, SKOV3, HCT116.

Predicted reactivity: Human (99%), Mouse (93%), Rat (94%), Pig (95%), Rhesus Monkey (99%), Bovine (83%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

SMYD3 (histone-lysine N-methyltransferase) methylates Lys-4 of histone H3 and Lys-5 of histone H4. It induces di- and tri-methylation. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: bA74P14.1 (novel protein); Histone-lysine N-methyltransferase SMYD3; SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing), 1; zinc finger, MYND domain containing 1

Gene Aliases: 2410008A19Rik; bA74P14.1; KMT3E; SMYD3; ZMYND1; ZNFN3A1

UniProt ID: (Human) Q9H7B4, (Mouse) Q9CWR2

Entrez Gene ID: (Human) 64754, (Mouse) 69726

Molecular Function: transcription cofactor transcription factor