Proteintech

SMYD4 Polyclonal Antibody

View all (7) SMYD4 antibodies

Questions & Answers

Tech Support

Questions & Answers

Tech Support

Antibody Testing Data (8)

SMYD4 Antibody in Immunocytochemistry (ICC/IF)

SMYD4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

SMYD4 Antibody (17594-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 17594-1-AP (SMYD4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SMYD4 Antibody in Immunocytochemistry (ICC/IF)

SMYD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMYD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMYD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMYD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMYD4 Antibody in Western Blot (WB)

SMYD4 Antibody in Western Blot (WB)

SMYD4 Antibody in Immunoprecipitation (IP)

Product Image Gallery

SMYD4 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 17594-1-AP (SMYD4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SMYD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human hepatocirrhosis using 17594-1-AP (SMYD4 antibody) at dilution of 1:100 (under 40x lens).

SMYD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human hepatocirrhosis using 17594-1-AP (SMYD4 antibody) at dilution of 1:100 (under 10x lens).

SMYD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas using 17594-1-AP (SMYD4 antibody) at dilution of 1:50 (under 40x lens).

SMYD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas using 17594-1-AP (SMYD4 antibody) at dilution of 1:50 (under 10x lens).

SMYD4 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 17594-1-AP (SMYD4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

SMYD4 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 17594-1-AP (SMYD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SMYD4 Antibody in Immunoprecipitation (IP) — IP result of anti-SMYD4 (IP:17594-1-AP, 4ug; Detection:17594-1-AP 1:500) with mouse brain tissue lysate 4000ug.

Product Details

17594-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SMYD4 Fusion Protein Ag11552 (201-578 aa encoded by BC035077)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KDSLTESFP AALAKTLEDA ALREENEQLS NASSSIGLCV DPLKGRCLVA TKDILPGELL VQEDAFVSVL NPGELPPPHH GLDSKWDTRV TNGDLYCHRC LKHTLATVPC DGCSYAKYCS QECLQQVWEL YHRTECPLGG LLLTLGVFCH IALRLTLLVG FEDVRKIITK LCDKISNKDI CLPESNNQVK TLNYGLGESE KNGNIVETPI PGCDINGKYE NNYNAVFNLL PHTENHSPEH KFLCALCVSA LCRQLEAASL QAIPTERIVN SSQLKAAVTP ELCPDVTIWG VAMLRHMLQL QCNAQAMTTI QHTGPKGSIV TDSRQVRLAT GIFPVISLLN HSCSPNTSVS FISTVATIRA SQRIRKGQEI LHCYGPHKS (201-578 aa encoded by BC035077)

Target Information

The specific function of the protein remains unknown.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The roles of SMYD4 in epigenetic regulation of cardiac development in zebrafish.

PLoS genetics

Xiao D,Wang H,Hao L,Guo X,Ma X,Qian Y,Chen H,Ma J,Zhang J,Sheng W,Shou W,Huang G,Ma D

Wed Aug 01 00:00:00 EDT 2018

The roles of SMYD4 in epigenetic regulation of cardiac development in zebrafish.

PLoS genetics

Xiao D,Wang H,Hao L,Guo X,Ma X,Qian Y,Chen H,Ma J,Zhang J,Sheng W,Shou W,Huang G,Ma D

Wed Aug 01 00:00:00 EDT 2018

The roles of SMYD4 in epigenetic regulation of cardiac development in zebrafish.

PLoS genetics

Xiao D,Wang H,Hao L,Guo X,Ma X,Qian Y,Chen H,Ma J,Zhang J,Sheng W,Shou W,Huang G,Ma D

Wed Aug 01 00:00:00 EDT 2018

The roles of SMYD4 in epigenetic regulation of cardiac development in zebrafish.

PLoS genetics

Xiao D,Wang H,Hao L,Guo X,Ma X,Qian Y,Chen H,Ma J,Zhang J,Sheng W,Shou W,Huang G,Ma D

Wed Aug 01 00:00:00 EDT 2018

The roles of SMYD4 in epigenetic regulation of cardiac development in zebrafish.

PLoS genetics

Xiao D,Wang H,Hao L,Guo X,Ma X,Qian Y,Chen H,Ma J,Zhang J,Sheng W,Shou W,Huang G,Ma D

Wed Aug 01 00:00:00 EDT 2018

The roles of SMYD4 in epigenetic regulation of cardiac development in zebrafish.

PLoS genetics

Xiao D,Wang H,Hao L,Guo X,Ma X,Qian Y,Chen H,Ma J,Zhang J,Sheng W,Shou W,Huang G,Ma D

Wed Aug 01 00:00:00 EDT 2018

The roles of SMYD4 in epigenetic regulation of cardiac development in zebrafish.

PLoS genetics

Xiao D,Wang H,Hao L,Guo X,Ma X,Qian Y,Chen H,Ma J,Zhang J,Sheng W,Shou W,Huang G,Ma D

Wed Aug 01 00:00:00 EDT 2018

The roles of SMYD4 in epigenetic regulation of cardiac development in zebrafish.

PLoS genetics

Xiao D,Wang H,Hao L,Guo X,Ma X,Qian Y,Chen H,Ma J,Zhang J,Sheng W,Shou W,Huang G,Ma D

Wed Aug 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: SET and MYND domain-containing protein 4

View more View less

Gene Aliases: G430029E23Rik; KIAA1936; SMYD4; ZMYND21

View more View less

UniProt ID: (Human) Q8IYR2, (Mouse) Q8BTK5

View more View less

Entrez Gene ID: (Human) 114826, (Mouse) 319822, (Rat) 287525

View more View less

methyltransferase activity metal ion binding rRNA (adenine-N6,N6-)-dimethyltransferase activity molecular_function protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity

Process(es)

biological_process methylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support