GenScript

SNAP-tag Polyclonal Antibody

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Antibody Testing Data (1)

FIGURE: 1 / 1

SNAP-tag Antibody (A00684-40) in WB

Lane 1-2: SNAP fusion protein in 293 cell lysate (~ 28 kD). Lane 3: Negative 293 cell lysate. Primary antibody: 1µg/mL Rabbit Anti-SNAP-tag Polyclonal Antibody (GenScript, A00684). Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000). The signal was developed with LumiSensor TM HRP Substrate Kit (GenScript, L00221500).

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Product Details

A00684-40

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1.0 µg/mL

ELISA (ELISA)

0.05-0.2 µg/mL

Miscellaneous PubMed (Misc)

View 6 publications 6 publications

Product Specifications

Species Reactivity

Tag

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified recombinant SNAP-tag fusion protein

Conjugate

Unconjugated

Form

Lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized antibody with deionized water to the final concentration of 0.5 mg/ml.

Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems. The SNAP-tag is a self-labeling protein tag that forms a highly stable, covalent thioether bond with fluorophores and other substituted groups on its benzyl guanine substrate. The expressed SNAP-tag fusion proteins can be used in many different assay formats depending upon the labeling substrate employed. GenScript Rabbit Anti-SNAP-tag Polyclonal Antibody is developed in rabbit hosts using purified recombinant SNAP-tag fusion protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

How GPCR Phosphorylation Patterns Orchestrate Arrestin-Mediated Signaling.

Cell

Latorraca NR,Masureel M,Hollingsworth SA,Heydenreich FM,Suomivuori CM,Brinton C,Townshend RJL,Bouvier M,Kobilka BK,Dror RO

Wed Dec 23 00:00:00 EST 2020

Differentiation syndrome during ivosidenib treatment with immunohistochemistry showing isocitrate dehydrogenase R132H mutation.

Journal of cutaneous pathology

Tabata MM,Chase M,Kwong BY,Novoa RA,Fernandez-Pol S

We report a case of differentiation syndrome in a patient receiving the IDH1 inhibitor ivosidenib, with skin biopsy showing isocitrate dehydrogenase (IDH) R132H-mutated leukemia cutis. A 72-year-old man with IDH1-mutated acute myeloid leukemia (AML), status-post allogeneic cell transplantation, on ivosidenib for six months, was admitted for culture-negative neutropenic fever, pink and purpuric plaques and patches on the legs, abdomen and back, edema, hypotension, and shortness of breath. Skin bi

Sun Nov 01 00:00:00 EDT 2020

Chromogranin B regulates early-stage insulin granule trafficking from the Golgi in pancreatic islet β-cells.

Journal of cell science

Bearrows SC,Bauchle CJ,Becker M,Haldeman JM,Swaminathan S,Stephens SB

Chromogranin B (CgB also known as CHGB) is abundantly expressed in dense core secretory granules of multiple endocrine tissues and has been suggested to regulate granule biogenesis in some cell types including the pancreatic islet β-cell though the mechanisms are poorly understood. Here we demonstrate a critical role for CgB in regulating secretory granule trafficking in the β-cell. Loss of CgB impairs glucose-stimulated insulin secretion impedes proinsulin processing to yield increase

Mon Jul 01 00:00:00 EDT 2019

A new cell line for high throughput HIV-specific antibody-dependent cellular cytotoxicity (ADCC) and cell-to-cell virus transmission studies.

Journal of immunological methods

Orlandi C,Flinko R,Lewis GK

Several lines of evidence indicate that antibody-dependent cellular cytotoxicity (Wren et al., 2013) is important in the pathogenesis of HIV-1 infection. Namely, ADCC is induced during natural HIV-1 infection or in HIV-1 vaccine studies, the latter demonstrated by the RV144 vaccine trial. To expedite the assessment of ADCC in studies of HIV, we have developed a high throughput assay. We have optimized the rapid fluorometric antibody-mediated cytotoxicity assay (RFADCC) by transfecting the EGFP-C

Wed Jun 01 00:00:00 EDT 2016

Phage display-based generation of novel internalizing antibody fragments for immunotoxin-based treatment of acute myeloid leukemia.

mAbs

Fitting J,Blume T,Ten Haaf A,Blau W,Gattenlöhner S,Tur MK,Barth S

The current standard treatment for acute myeloid leukemia (AML) is chemotherapy based on cytarabine and daunorubicine (7 + 3), but it discriminates poorly between malignant and benign cells. Dose-limiting off target effects and intrinsic drug resistance result in the inefficient eradication of leukemic blast cells and their survival beyond remission. This minimal residual disease is the major cause of relapse and is responsible for a 5-year survival rate of only 24%. More specific and efficient

Mon Nov 30 00:00:00 EST 2015

Recombinant human cytoplasmic dynein heavy chain 1 and 2: observation of dynein-2 motor activity in vitro.

FEBS letters

Ichikawa M,Watanabe Y,Murayama T,Toyoshima YY

Cytoplasmic dynein is a microtubule (MT) motor protein comprising two classes: dynein-1 and dynein-2. We purified recombinant human dynein-1 and dynein-2 from HEK-293 cells by expressing the streptavidin-binding peptide-tagged human cytoplasmic dynein-1 and dynein-2 heavy chains (HCs), respectively. Electron microscopy of the purified molecules revealed a two-headed structure composed of characteristic dynein motor domains. In an in vitro MT gliding assay, both dynein-1 and dynein-2 showed minus

Thu Aug 04 00:00:00 EDT 2011

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