Proteintech

SNAP23 Polyclonal Antibody

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SNAP23 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using SNAP23 antibody 10825-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

SNAP23 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human placenta tissue slide using 10825-1-AP (SNAP23 Antibody) at dilution of 1:50 (under 40x lens).

SNAP23 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 10825-1-AP (SNAP23 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SNAP23 Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug SNAP23 antibody (10825-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

SNAP23 Antibody in Immunoprecipitation (IP) — IP result of anti-SNAP23 (IP:10825-1-AP, 4ug; Detection:10825-1-AP 1:500) with HeLa cells lysate 880ug.

Product Details

10825-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Goat, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SNAP23 Fusion Protein Ag1269 (1-211 aa encoded by BC000148) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.57 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MDNLSSEEI QQRAHQITDE SLESTRRILG LAIESQDAGI KTITMLDEQK EQLNRIEEGL DQINKDMRET EKTLTELNKC CGLCVCPCNR TKNFESGKAY KTTWGDGGEN SPCNVVSKQP GPVTNGQLQQ PTTGAASGGY IKRITNDARE DEMEENLTQV GSILGNLKDM ALNIGNEIDA QNPQIKRITD KADTNRDRID IANARAKKLI DS (1-211 aa encoded by BC000148)

Target Information

Specificity of vesicular transport is regulated, in part, by the interaction of a vesicle-associated membrane protein termed synaptobrevin/VAMP with a target compartment membrane protein termed syntaxin. These proteins, together with SNAP25 (synaptosome-associated protein of 25 kDa), form a complex which serves as a binding site for the general membrane fusion machinery. Synaptobrevin/VAMP and syntaxin are believed to be involved in vesicular transport in most, if not all cells, while SNAP25 is present almost exclusively in the brain, suggesting that a ubiquitously expressed homolog of SNAP25 exists to facilitate transport vesicle/target membrane fusion in other tissues. The protein encoded by this gene is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion. Two alternative transcript variants encoding different protein isoforms have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Comparison of the Expression Changes after Botulinum Toxin Type A and Minocycline Administration in Lipopolysaccharide-Stimulated Rat Microglial and Astroglial Cultures.

Frontiers in cellular and infection microbiology

Piotrowska A,Popiolek-Barczyk K,Pavone F,Mika J

Wed Dec 27 00:00:00 EST 2017

Ultrastructure and immunohistochemical characterization of proteins concerned with the secretory machinery in goat ceruminous glands.

European journal of histochemistry : EJH

Yasui T,Gomi H,Kitahara T,Tsukise A

Tue Aug 08 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: OTTHUMP00000161263; SNAP-23; Synaptosomal-associated protein 23; synaptosomal-associated protein 23 kD; synaptosomal-associated protein, 23 kD; synaptosomal-associated protein, 23kD; synaptosomal-associated protein, 23kDa; synaptosome associated protein 23kDa; Syndet; Vesicle-membrane fusion protein SNAP-23

Gene Aliases: 23kDa; AA408749; HsT17016; SNAP-23; SNAP23; SNAP23A; SNAP23B; Sndt; Syndet

UniProt ID: (Human) O00161, (Mouse) O09044, (Rat) O70377

Entrez Gene ID: (Human) 8773, (Mouse) 20619, (Rat) 64630

Molecular Function: SNARE protein

Function(s)

histamine secretion by mast cell post-Golgi vesicle-mediated transport vesicle targeting protein transport synaptic vesicle priming synaptic vesicle fusion to presynaptic active zone membrane membrane fusion protein complex assembly transport exocytosis vesicle-mediated transport regulation of exocytosis

Process(es)

SNAP receptor activity protein binding syntaxin binding

SNAP23 Polyclonal Antibody

Product Details

10825-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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