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Invitrogen
SNAP29 Polyclonal Antibody
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FIGURE: 1 / 2
SNAP29 Antibody (PA5-96282) in WB
Western blot analysis of SNAP29 in HeLa cells. Samples were incubated with SNAP29 Polyclonal antibody (Product # PA5-96282) using a dilution of 1:700, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-96282
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human SNAP29 (NP_0047731)
Conjugate
Form
Liquid
Concentration
0.93 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MSAYPKSYNP FDDDGEDEGA RPAPWRDARD LPDGPDAPAD RQQYLRQEVL RRAEATAAST SRSLALMYES EKVGVASSEE LARQRGVLER TEKMVDKMDQ DLKISQKHIN SIKSVFGGLV NYFKSKPVET PPEQNGTLTS QPNNRLKEAI STSKEQEAKY QASHPNLRKL DDTDPVPRGA GSAMSTDAYP KNPHLRAYHQ KIDSNLDELS MGLGRLKDIA LGMQTEIEEQ DDILDRLTTK VDKLDVNIKS TERKVRQL; Positive Samples: A-549, HeLa, BxPC-3, HepG2, 293T, U-251MG; Cellular Location: Cell projection, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus membrane, Peripheral membrane protein, autophagosome membrane, cilium membrane
Target Information
SNAP29 encodes a product that is a member of the SNAP25 gene family, encodes a protein involved in multiple membrane trafficking steps. Two other members of this gene family, SNAP23 and SNAP25, encode proteins that bind a syntaxin protein and mediate synaptic vesicle membrane docking and fusion to the plasma membrane. The protein encoded by this gene binds tightly to multiple syntaxins and is localized to intracellular membrane structures rather than to the plasma membrane. While the protein is mostly membrane-bound, a significant fraction of it is found free in the cytoplasm. Use of multiple polyadenylation sites has been noted for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ21051; Golgi SNARE of 32 kDa; Gs32; SNAP-29; Soluble 29 kDa NSF attachment protein; Synaptosomal-associated protein 29; synaptosomal-associated protein, 29kD; synaptosomal-associated protein, 29kDa; synaptosome associated protein 29kDa; Vesicle-membrane fusion protein SNAP-29
Gene Aliases: 1300018G05Rik; AI891940; AU020222; BB131856; CEDNIK; Gs32; SNAP-29; SNAP29
UniProt ID: (Human) O95721, (Mouse) Q9ERB0
Entrez Gene ID: (Human) 9342, (Mouse) 67474
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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