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Invitrogen
SNF1 Polyclonal Antibody
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FIGURE: 1 / 1
SNF1 Antibody (PA5-144419) in WB
Western blot analysis of SNF1 in recombinant protein using a dilution of 1:2,000. Samples were incubated with SNF1 polyclonal antibody (Product # PA5-144419). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 27 kDa. Observed band size: 36 kDa.
Product Details
PA5-144419
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Yeast
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508/S288c) (Baker's yeast) SNF1 protein (435-633aa)
Conjugate
Form
Liquid
Concentration
3.91 mg/mL
Amount
391 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Serine/threonine protein kinase essential for release from glucose repression (PubMed:3526554, PubMed:6366512, PubMed:3049551, PubMed:1944227, PubMed:8289797, PubMed:8628258, PubMed:25869125). Catalytic subunit of the AMP-activated protein kinase complex also known as the SNF1 kinase complex (Snf1c), a central regulator of cellular energy homeostasis, which, in response to a fall in intracellular ATP levels, activates energy-producing pathways and inhibits energy-consuming processes (PubMed:8289797, PubMed:26667037). The complex phosphorylates histone H3 to form H3S10ph, which promotes H3K14ac formation, leading to transcriptional activation through TBP recruitment to the promoters (PubMed:15719021). The complex also negatively regulates the HOG1 MAPK pathway in ER stress response including unfolded protein response (UPR) (PubMed:25730376, PubMed:26394309). Under nutrient/energy depletion, the complex phosphorylates and activates PAS kinase PSK1 which in turn activates PBS1, leading to the inhibition of the TORC1 signaling pathway (PubMed:25428989). SNF1 also interacts and phosphorylates adenylate cyclase CYR1 and negatively regulates the protein kinase A signaling pathway (PubMed:26309257). Also phosphorylates and regulates the transcriptional activator CAT8 (PubMed:15121831).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Carbon catabolite-derepressing protein kinase; CAT1; CCR1; GLC2; PAS14; Sucrose nonfermentating protein 1
Gene Aliases: CAT1; CCR1; D8035.20; GLC2; HAF3; PAS14; SNF1; YDR477W
UniProt ID: (Baker's yeast) P06782
Entrez Gene ID: (Baker's yeast) 852088
establishment of mitotic spindle orientation
invasive growth in response to glucose limitation
carbohydrate metabolic process
protein phosphorylation
response to unfolded protein
cellular response to nitrogen starvation
cell adhesion
positive regulation of macroautophagy
phosphorylation
negative regulation of translation
response to endoplasmic reticulum stress
intracellular signal transduction
positive regulation of gluconeogenesis
regulation of catalytic activity
fungal-type cell wall assembly
single-species surface biofilm formation
positive regulation of filamentous growth of a population of unicellular organisms in response to starvation
positive regulation of pseudohyphal growth
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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