Proteintech

SNX6 Polyclonal Antibody

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SNX6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 40x lens).

SNX6 Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

SNX6 Antibody in Flow Cytometry (Flow) — 1X10^6 RAW 264.7 cells were stained with 0.2ug SNX6 antibody (10114-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

SNX6 Antibody in Immunoprecipitation (IP) — IP result of anti-SNX6 (IP:10114-1-AP, 4ug; Detection:10114-1-AP 1:600) with HepG2 cells lysate 4000ug.

Product Details

10114-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SNX6 Fusion Protein Ag0168 (19-290 aa encoded by BC001798)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: AIFKKTVAM HEVFLCRVAA HPILRRDLNF HVFLEYNQDL SVRGKNKKEK LEDFFKNMVK SADGVIVSGV KDVDDFFEHE RTFLLEYHNR VKDASAKSDR MTRSHKSAAD DYNRIGSSLY ALGTQDSTDI CKFFLKVSEL FDKTRKIEAR VSADEDLKLS DLLKYYLRES QAAKDLLYRR SRSLVDYENA NKALDKARAK NKDVLQAETS QQLCCQKFEK ISESAKQELI DFKTRRVAAF RKNLVELAEL ELKHAKGNLQ LLQNCLAVLN GDT (19-290 aa encoded by BC001798)

Target Information

SNX6 interacts with members of the transforming growth factor-beta family of receptor serine-threonine kinases. These receptors belong to two classes: type II receptors that bind ligand, and type I receptors that are subsequently recruited to transduce the signal. Of the type II receptors, SNX6 was found to interact strongly with ActRIIB and more moderately with wild type and kinase-defective mutants of TbetaRII. Of the type I receptors, SNX6 was found to interact only with inactivated TbetaRI. SNXs 1-4 also interacted with the transforming growth factor-beta receptor family, showing different receptor preferences. Conversely, SNX6 behaved similarly to the other SNX proteins in its interactions with receptor tyrosine kinases. Strong heteromeric interactions were also seen among SNX1, -2, -4, and -6, suggesting the formation in vivo of oligomeric complexes. These findings are the first evidence for the association of the SNX family of molecules with receptor serine-threonine kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Recycling of autophagosomal components from autolysosomes by the recycler complex.

Nature cell biology

Zhou C,Wu Z,Du W,Que H,Wang Y,Ouyang Q,Jian F,Yuan W,Zhao Y,Tian R,Li Y,Chen Y,Gao S,Wong CCL,Rong Y

Fri Apr 01 00:00:00 EDT 2022

SNX6 predicts poor prognosis and contributes to the metastasis of pancreatic cancer cells via activating epithelial-mesenchymal transition.

Acta biochimica et biophysica Sinica

Hu P,Liang Y,Hu Q,Wang H,Cai Z,He J,Cai J,Liu M,Qin Y,Yu X,Jiang C,Zhang B,Wang W

Thu Nov 01 00:00:00 EDT 2018

SNX6 predicts poor prognosis and contributes to the metastasis of pancreatic cancer cells via activating epithelial-mesenchymal transition.

Acta biochimica et biophysica Sinica

Hu P,Liang Y,Hu Q,Wang H,Cai Z,He J,Cai J,Liu M,Qin Y,Yu X,Jiang C,Zhang B,Wang W

Thu Nov 01 00:00:00 EDT 2018

SNX6 predicts poor prognosis and contributes to the metastasis of pancreatic cancer cells via activating epithelial-mesenchymal transition.

Acta biochimica et biophysica Sinica

Hu P,Liang Y,Hu Q,Wang H,Cai Z,He J,Cai J,Liu M,Qin Y,Yu X,Jiang C,Zhang B,Wang W

Thu Nov 01 00:00:00 EDT 2018

SNX6 predicts poor prognosis and contributes to the metastasis of pancreatic cancer cells via activating epithelial-mesenchymal transition.

Acta biochimica et biophysica Sinica

Hu P,Liang Y,Hu Q,Wang H,Cai Z,He J,Cai J,Liu M,Qin Y,Yu X,Jiang C,Zhang B,Wang W

Thu Nov 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Sorting nexin-6; Sorting nexin-6, N-terminally processed; TRAF4-associated factor 2; tumor necrosis factor receptor-associated factor 4(TRAF4)-associated factor 2

Gene Aliases: 2010006G21Rik; 2610032J07Rik; 2810425K19Rik; AU018928; C85963; MSTP010; SNX6; TFAF2

UniProt ID: (Human) Q9Y449, (Mouse) Q6P8X1

Entrez Gene ID: (Human) 58533, (Mouse) 72183, (Rat) 362738

Molecular Function: scaffold/adaptor protein