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Invitrogen
SOAT2 Polyclonal Antibody
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FIGURE: 1 / 3
SOAT2 Antibody (PA5-100373) in ICC/IF
Immunocytochemistry analysis of SOAT2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SOAT2 polyclonal antibody (Product # PA5-100373) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100373
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SOAT2(Accession O75908), corresponding to amino acid residues V475-T522.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total ACAT2.
Target Information
This gene is a member of a small family of acyl coenzyme A:cholesterol acyltransferases. The gene encodes a membrane-bound enzyme localized in the endoplasmic reticulum that produces intracellular cholesterol esters from long-chain fatty acyl CoA and cholesterol. The cholesterol esters are then stored as cytoplasmic lipid droplets inside the cell. The enzyme is implicated in cholesterol absorption in the intestine and in the assembly and secretion of apolipoprotein B-containing lipoproteins such as very low density lipoprotein (VLDL). Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ACAT-2; ACAT2; acyl Co-A: cholesterol acyltransferase 2; acyl coenzyme A:cholesterol acyltransferase 2; acyl-CoA:cholesterol acyltransferase; acyl-CoA:cholesterol acyltransferase 2; Acyl-coenzyme A:cholesterol acyltransferase 2; Cholesterol acyltransferase 2; Sterol O-acyltransferase 2
Gene Aliases: Acact-2; ACACT2; Acat-2; ACAT2; ARGP2; D15Wsu97e; SOAT2
UniProt ID: (Human) O75908, (Rat) Q7TQM4, (Mouse) O88908
Entrez Gene ID: (Human) 8435, (Rat) 266770, (Mouse) 223920
lipid metabolic process
steroid metabolic process
cholesterol metabolic process
macrophage derived foam cell differentiation
cholesterol storage
intestinal cholesterol absorption
cholesterol efflux
very-low-density lipoprotein particle assembly
low-density lipoprotein particle clearance
cholesterol homeostasis
response to nutrient
cholesterol esterification
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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