Immunohistochemistry analysis of SOCS-1 showing staining in the cytoplasm and nucleus of paraffin-embedded human stomach carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- SOCS-1 Polyclonal Antibody (385200) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Raised against the C-term of human SOCS1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
The Suppressor of cytokine signaling (SOCS) and cytokine-inducible SH2 proteins are a family of intracellular proteins which regulate the immune cell responses to cytokines. SOCS1 acts to suppress dendritic cell (DC) as well as T cell hyperactivation following cytokine signaling by inhibiting JAK tyrosine kinase, a kinase necessary for type I and II cytokine receptors to initiate signaling, by directly binding to the catalytic domain of the kinase. SOCS1 also possesses E3 ubiquitin protein ligase activity that results in the polyubiquitination of its target proteins and subsequent degradation by the proteosome. It is through this method that SOCS1 negatively regulates signaling by Toll-like receptors TLR2 and TLR4 by mediating the degradation of the TLR signaling adaptor protein TIRAP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
1,25-Dihydroxyvitamin D promotes negative feedback regulation of TLR signaling via targeting microRNA-155-SOCS1 in macrophages.
38-5200 was used in western blot to demonstrate that vitamin D receptor signaling attenuates TLR-mediated inflammation.
|Chen Y,Liu W,Sun T,Huang Y,Wang Y,Deb DK,Yoon D,Kong J,Thadhani R,Li YC||Journal of immunology (Baltimore, Md. : 1950) (190:3687)||2013|
|Not Applicable||Not Cited||
Stage dependent aberrant regulation of cytokine-STAT signaling in murine systemic lupus erythematosus.
38-5200 was used in western blot to observe how the STAT family of transcription factors is altered during the progression of systemic lupus erythematosus
|Hale MB,Krutzik PO,Samra SS,Crane JM,Nolan GP||PloS one (4:null)||2009|