|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide VLRDYLSSFPFQI, corresponding to amino acids 199-211of human SOCS 1 or 200-212 of the mouse SOCS 1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1 µg/ml|
|Immunoprecipitation (IP)||3-5 µg x 10^7 cells|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29533 detects SOCS1 from human samples.
PA1-29533 has been successfully used in immunoprecipitation, Western blot and ELISA applications. By Western blot, a band of ~24kDa is detedted in GH-treated HEK 293 cell lysates.
The PA1-29533 immunogen is: Synthetic peptide: VLRDYLSSFPFQI, corresponding to amino acids 199-211of human SOCS 1 or 200-212 of the mouse SOCS 1.
Accumulating evidence has demonstrated that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family that have been identified; they are SOCS 1, 2, 3, 4, 5, 6, 7 and CIS. Structurally, the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif named the SOCS box. SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction via a STAT-induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines. Once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS 1 inhibition of signal transduction appears to occur by binding to and inhibiting the catalytic activity of members of JAK family of cytoplasmic kinases (direct JAK2 kinas inhibition). Biochemical characterization as well as gene disruption studies indicate that SOCS 1 is an important negative regulator of JAK-STAT signal pathway. SOCS 1 is also called JAB SSI 1 or TIP3 (Tec-Interacting Protein).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.