Immunohistochemical analysis of SOCS2 in cardiac myocytes using a SOCS2 polyclonal antibody (Product # PA1-28393). The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain in cardiac myocytes of the heart.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (PTRLKDYLEEYKFQV) correspondingto amino acids 184-198 of human, rat and mouse SOCS 2.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1 µg/ml|
|Immunoprecipitation (IP)||3-5 µg x 10^7 cells|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28393 detects SOCS2 from mouse, rat, human samples.
PA1-28393 has been successfully used in immunoprecipitation, Western blot applications.
The PA1-28393 immunogen is: Synthetic peptide (PTRLKDYLEEYKFQV) correspondingto amino acids 184-198 of human, rat and mouse SOCS 2.
This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including erythropoietin, GM-CSF, IL10 and interferon (IFN)-gamma. The protein encoded by this gene is found to interact with the cytoplasmic domain of insulin-like growth factor-1 receptor (IGF1R), and thus is thought to be involved in the regulation of IGF1R mediated cell signaling. Knockout studies in mice also suggested a regulatory role of this gene in IGF-1 related growth control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.