Proteintech

SOCS3 Polyclonal Antibody

Product Image Gallery

SOCS3 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of HepG2 cells, using SOCS3 antibody 14025-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

SOCS3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart tissue slide using 14025-1-AP (SOCS3 antibody) at dilution of 1:200 (under 10x lens).

SOCS3 Antibody in Western Blot (WB) — WB result of SOCS3 antibody (14025-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOCS3 transfected HeLa cells.

SOCS3 Antibody in Immunoprecipitation (IP) — IP result of anti-SOCS3 (IP:14025-1-AP, 4ug; Detection:14025-1-AP 1:500) with K-562 cells lysate 1720ug.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:10-1:100

Immunofluorescence (IF)

1:10-1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:1000

Published Applications

Western Blot (WB)

See 17 publications below

Immunohistochemistry (IHC)

See 3 publications below

Immunofluorescence (IF)

See 1 publication below

Miscellaneous PubMed (MISC)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SOCS3 Fusion Protein Ag5170

Conjugate

Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

Accumulating evidence demonstrates that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules Termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have been recognized, they are SOCS-1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT-induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ATOD4; CIS 3; CIS-3; CIS3; Cish3; cytokine inducible SH2-containing protein 3; Cytokine-inducible SH2 protein 3; E2a-Pbx1 target gene in fibroblasts 10; Protein EF-10; SOCS 3; SOCS-3; SOCS3; SSI 3; SSI-3; SSI3; STAT induced STAT inhibitor 3; STAT-induced STAT inhibitor 3; Suppressor of cytokine signaling 3

Gene Aliases: ATOD4; CIS3; Cish3; EF-10; Ef10; SOCS-3; SOCS3; SSI-3; SSI3

UniProt ID: (Human) O14543, (Mouse) O35718

Entrez Gene ID: (Human) 9021, (Mouse) 12702

Molecular Function: cytokine signaling molecule