Proteintech

SOCS3 Polyclonal Antibody

Product Image Gallery

SOCS3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using SOCS3 antibody 14025-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

SOCS3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart tissue slide using 14025-1-AP (SOCS3 antibody) at dilution of 1:200 (under 40x lens).

SOCS3 Antibody in Western Blot (WB) — WB result of SOCS3 antibody (14025-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOCS3 transfected HeLa cells.

SOCS3 Antibody in Immunoprecipitation (IP) — IP result of anti-SOCS3 (IP:14025-1-AP, 4ug; Detection:14025-1-AP 1:500) with K-562 cells lysate 1720ug.

Product Details

14025-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 17 publications 17 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SOCS3 Fusion Protein Ag5170
Conjugate	Unconjugated
Form	Liquid
Concentration	0.23 mg/mL
Storage conditions	-20°C

Target Information

Accumulating evidence demonstrates that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules Termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have been recognized, they are SOCS-1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT-induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transcriptional factor EB regulates macrophage polarization in the tumor microenvironment.

Oncoimmunology

Fang L,Hodge J,Saaoud F,Wang J,Iwanowycz S,Wang Y,Hui Y,Evans TD,Razani B,Fan D

Tue Jan 12 00:00:00 EST 2021

Porphyromonas gingivalis and its lipopolysaccharide differently modulate epidermal growth factor-dependent signaling in human gingival epithelial cells.

Journal of oral microbiology

Elkaim R,Bugueno-Valdebenito IM,Benkirane-Jessel N,Tenenbaum H

Wed Sep 30 00:00:00 EDT 2020

Neonatal Overnutrition Increases Testicular Size and Expression of Luteinizing Hormone β-Subunit in Peripubertal Male Rats.

Frontiers in endocrinology

Argente-Arizón P,Castro-González D,Díaz F,Fernández-Gómez MJ,Sánchez-Garrido MA,Tena-Sempere M,Argente J,Chowen JA

Wed Nov 20 00:00:00 EST 2019

MBD2-mediated Th17 differentiation in severe asthma is associated with impaired SOCS3 expression.

Experimental cell research

Sun W,Xiao B,Jia A,Qiu L,Zeng Q,Liu D,Yuan Y,Jia J,Zhang X,Xiang X

Mon Oct 01 00:00:00 EDT 2018

Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats.

Behavioural brain research

Lian S,Wang D,Xu B,Guo W,Wang L,Li W,Ji H,Wang J,Kong F,Zhen L,Li S,Zhang L,Guo J,Yang H

Mon Jul 02 00:00:00 EDT 2018

Di-(2-ethylhexyl)-phthalate induces glucose metabolic disorder in adolescent rats.

Environmental science and pollution research international

Xu J,Zhou L,Wang S,Zhu J,Liu T,Jia Y,Sun D,Chen H,Wang Q,Xu F,Zhang Y,Liu H,Zhang T,Ye L

Thu Feb 01 00:00:00 EST 2018

Di-(2-ethylhexyl)-phthalate induces glucose metabolic disorder in adolescent rats.

Environmental science and pollution research international

Xu J,Zhou L,Wang S,Zhu J,Liu T,Jia Y,Sun D,Chen H,Wang Q,Xu F,Zhang Y,Liu H,Zhang T,Ye L

Thu Feb 01 00:00:00 EST 2018

The Hypothalamic Inflammatory/Gliosis Response to Neonatal Overnutrition Is Sex and Age Dependent.

Endocrinology

Argente-Arizón P,Díaz F,Ros P,Barrios V,Tena-Sempere M,García-Segura LM,Argente J,Chowen JA

Mon Jan 01 00:00:00 EST 2018

Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.

Oncotarget

Igbe I,Shen XF,Jiao W,Qiang Z,Deng T,Li S,Liu WL,Liu HW,Zhang GL,Wang F

Tue Dec 26 00:00:00 EST 2017

6-Hydroxy-3-O-methyl-kaempferol 6-O-glucopyranoside potentiates the anti-proliferative effect of interferon α/β by promoting activation of the JAK/STAT signaling by inhibiting SOCS3 in hepatocellular carcinoma cells.

Toxicology and applied pharmacology

Wonganan O,He YJ,Shen XF,Wongkrajang K,Suksamrarn A,Zhang GL,Wang F

Fri Dec 01 00:00:00 EST 2017

MiR-155 Enhances Insulin Sensitivity by Coordinated Regulation of Multiple Genes in Mice.

PLoS genetics

Lin X,Qin Y,Jia J,Lin T,Lin X,Chen L,Zeng H,Han Y,Wu L,Huang S,Wang M,Huang S,Xie R,Liang L,Liu Y,Liu R,Zhang T,Li J,Wang S,Sun P,Huang W,Yao K,Xu K,Du T,Xiao D

Sat Oct 01 00:00:00 EDT 2016

Suppressors of cytokine signaling 3 is essential for FcγR-mediated inflammatory response via enhancing CCAAT/enhancer-binding protein δ transcriptional activity in macrophages.

Experimental cell research

Yan C,Liu Y,Gao H,Wang X

Thu Sep 10 00:00:00 EDT 2015

IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation.

BMC pulmonary medicine

Dong Z,Lu X,Yang Y,Zhang T,Li Y,Chai Y,Lei W,Li C,Ai L,Tai W

Wed Feb 18 00:00:00 EST 2015

Ablation of proximal tubular suppressor of cytokine signaling 3 enhances tubular cell cycling and modifies macrophage phenotype during acute kidney injury.

Kidney international

Susnik N,Sörensen-Zender I,Rong S,von Vietinghoff S,Lu X,Rubera I,Tauc M,Falk CS,Alexander WS,Melk A,Haller H,Schmitt R

Sun Jun 01 00:00:00 EDT 2014

Ablation of proximal tubular suppressor of cytokine signaling 3 enhances tubular cell cycling and modifies macrophage phenotype during acute kidney injury.

Kidney international

Susnik N,Sörensen-Zender I,Rong S,von Vietinghoff S,Lu X,Rubera I,Tauc M,Falk CS,Alexander WS,Melk A,Haller H,Schmitt R

Sun Jun 01 00:00:00 EDT 2014

Kallikrein-binding protein inhibits LPS-induced TNF-α by upregulating SOCS3 expression.

Journal of cellular biochemistry

Dai Z,Lu L,Yang Z,Mao Y,Lu J,Li C,Qi W,Chen Y,Yao Y,Li L,Chen S,Zhang Y,Cai W,Yang X,Gao G

Wed May 01 00:00:00 EDT 2013

Kallikrein-binding protein inhibits LPS-induced TNF-α by upregulating SOCS3 expression.

Journal of cellular biochemistry

Dai Z,Lu L,Yang Z,Mao Y,Lu J,Li C,Qi W,Chen Y,Yao Y,Li L,Chen S,Zhang Y,Cai W,Yang X,Gao G

Wed May 01 00:00:00 EDT 2013

TLR2-dependent selective autophagy regulates NF-κB lysosomal degradation in hepatoma-derived M2 macrophage differentiation.

Cell death and differentiation

Chang CP,Su YC,Hu CW,Lei HY

Fri Mar 01 00:00:00 EST 2013

Role of suppressor of cytokine signaling 3 in lipid metabolism: analysis based on a phage-display human liver cDNA library.

Biochemical and biophysical research communications

Luo B,Zou T,Lu N,Chai F,Ye X,Wang Y,Qi Y

Fri Dec 09 00:00:00 EST 2011

The role of microtubule-associated protein 1S in SOCS3 regulation of IL-6 signaling.

FEBS letters

Zou T,Ouyang L,Chen L,Dong W,Qiao H,Liu Y,Qi Y

Wed Dec 10 00:00:00 EST 2008

The role of microtubule-associated protein 1S in SOCS3 regulation of IL-6 signaling.

FEBS letters

Zou T,Ouyang L,Chen L,Dong W,Qiao H,Liu Y,Qi Y

Wed Dec 10 00:00:00 EST 2008

BCL10 mediates lipopolysaccharide/toll-like receptor-4 signaling through interaction with Pellino2.

The Journal of biological chemistry

Liu Y,Dong W,Chen L,Xiang R,Xiao H,De G,Wang Z,Qi Y

Fri Sep 03 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: CIS-3; cytokine inducible SH2-containing protein 3; Cytokine-inducible SH2 protein 3; E2a-Pbx1 target gene in fibroblasts 10; MGC71791; Protein EF-10; SOCS-3; SSI-3; STAT-induced STAT inhibitor 3; Suppressor of cytokine signaling 3

Gene Aliases: CIS3; Cish3; SOCS3; SSI3