Immunohistochemical analysis of SOCS3 in cardiac myocytes using a SOCS3 polyclonal antibody (Product # PA1-29534). The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain in cardiac myocytes of the heart.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (QLPGPIREFLDQYDAPL)corresponding to amino acids 209-225 of thehuman / rat / mouse SOCS 3.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1 µg/ml|
|Immunoprecipitation (IP)||3-5 µg x 10^7 cells|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29534 detects SOCS3 from rat, mouse, human samples.
PA1-29534 has been successfully used in ELISA, immunoprecipitation, Western blot applications.
The PA1-29534 immunogen is: Synthetic peptide (QLPGPIREFLDQYDAPL)corresponding to amino acids 209-225 of thehuman / rat / mouse SOCS 3.
This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.