Invitrogen

SOCS6 Polyclonal Antibody

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Antibody Testing Data (1)
Published Figures (2)

SOCS6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 3

SOCS6 Antibody (PA5-50263) in IHC (P)

Immunohistochemical analysis of SOCS6 in paraffin-embedded Human liver cancer tissue using SOCS6 Polyclonal Antibody (Product # PA5-50263) at a 1:50 dilution. (Original magnification: x200).

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SOCS6 Antibody in Western Blot (WB) — EV2 Figure MiR-342-3p directly targets SOCS6 to regulate anti-Mtb immunity A Relative expressions of miR-342-2p target genes were analyzed by qRT-PCR. Data are shown as the mean +- SEM of n = 3 biological replicates. B Cell death mechanisms of RAW264.7 cells transfected with siRNA, followed by Mtb infection for 36 h. Representative data (from n = 3 biological replicates) are shown as the mean +- SEM of technical replicates. C, D MiR-342-3p mimic or inhibitor was transfected to RAW264.7 macrophages. After 48 h, cells were collected for qRT-PCR (C, data are shown as the mean +- SEM of n = 3 biological replicates) and Western blotting (D, representative blots from n = 3 biological replicates are shown) to detect the relative levels of SOCS6. E C3H BMDMs were transfected with miR-342-3p mimic or SOCS6-overexpressing lentivirus, followed by Mtb infection. Cell death mechanisms were analyzed, respectively. Representative data (from n = 3 biological replicates) are shown as the mean +- SEM of technical replicates. F B6 BMDMs were transfected with miR-342-3p inhibitor or Socs6 siRNA, followed by Mtb infection. Cell death mechanisms were analyzed, respectively. Representative data (from n = 3 biological replicates) are shown as the mean +- SEM of technical replicates. G, H SOCS6-overexpressing vector (G) or Socs6 siRNA (H) was mixed with polyethylenimine to form a complex, which was used to infect mice by tail vein injection ( N /P ratio=8). Lungs were collected for transfection effic

Product Details

PA5-50263

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Fusion protein corresponding to a region derived from internal residues of human suppressor of cytokine signaling 6

Conjugate

Unconjugated

Form

Liquid

Concentration

0.7 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 40% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

RRID

AB_2635716

Product Specific Information

The antibody detects endogenous levels of total SOCS-6 protein.

Target Information

The eight members of the recently identified Suppressor of Cytokines Signaling (SOCS) family are SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS6, SOCS7, and CIS. Structurally the SOCS proteins are composed of an N- terminal region of variable length and amino acid composition, a central SH2 domain, and a C-terminal motif called the SOCS box. The SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS1 and SOCS6 interact with the insulin receptor (IR) when expressed in human hepatoma cells (HepG2) or in rat hepatoma cells overexpressing human IR. SOCS1 and SOCS6 inhibit insulin-dependent activation of ERK1/2 and protein kinase B in vivo and IR- directed phosphorylation of IRS1 in vitro. These results suggest that SOCS proteins may be inhibitors of IR signalling and could mediate cytokine-induced insulin resistance and contribute to the pathogenesis of type II diabetes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MiR-342 controls Mycobacterium tuberculosis susceptibility by modulating inflammation and cell death.

EMBO reports

Fu B,Lin X,Tan S,Zhang R,Xue W,Zhang H,Zhang S,Zhao Q,Wang Y,Feldman K,Shi L,Zhang S,Nian W,Chaitanya Pavani K,Li Z,Wang X,Wu H

Published figure using SOCS6 polyclonal antibody (Product # PA5-50263) in Western Blot

Mon Sep 06 00:00:00 EDT 2021

A short deletion in the DNA-binding domain of STAT3 suppresses growth and progression of colon cancer cells.

Aging

Xiong YJ,Liu DY,Shen RR,Xiong Y

Published figure using SOCS6 polyclonal antibody (Product # PA5-50263) in Western Blot

Mon Feb 01 00:00:00 EST 2021

Bioinformatics

Protein Aliases: CIS-4; cytokine inducible SH2-containing protein 4; cytokine inducible SH2-containing protein CIS4; Cytokine-inducible SH2 protein 4; SH2 domain containing SOCS box protein SOCS6; SOCS-4; SOCS-6; STAT induced STAT inhibitor-4; Suppressor of cytokine signaling 4; Suppressor of cytokine signaling 6

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Gene Aliases: 1500012M23Rik; 5830401B18Rik; AI447482; CIS-4; CIS4; Cish4; HSPC060; SOCS-4; SOCS-6; SOCS4; SOCS6; SSI4; STAI4; STATI4

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UniProt ID: (Human) O14544, (Mouse) Q9JLY0

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Entrez Gene ID: (Human) 9306, (Mouse) 54607

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Molecular Function: kinase modulator defense/immunity protein

Function(s)

negative regulation of protein kinase activity defense response JAK-STAT cascade proteasomal protein catabolic process protein ubiquitination cytokine-mediated signaling pathway regulation of growth negative regulation of JAK-STAT cascade negative regulation of T cell activation cellular glucose homeostasis negative regulation of signal transduction intracellular signal transduction

Process(es)

protein kinase inhibitor activity protein binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SOCS6 Polyclonal Antibody

SOCS6 Antibody (PA5-50263) in IHC (P)

Product Details

PA5-50263

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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