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Invitrogen
SOD1 Polyclonal Antibody
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FIGURE: 1 / 14
SOD1 Antibody (PA5-27240) in WB
Western blot analysis was performed on whole cell extracts of HEK-293 (Lane 1), HEK-293 treated with H2O2 (10uM, 6hrs) (Lane 2), HeLa (Lane 3), MCF-7 (Lane 4), and SH-SY5Y (Lane 5). The blot was probed with SOD1 Polyclonal Antibody (Product # PA5-27240, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 18kDa band corresponding to SOD1 was observed across the panel and was upregulated upon H2O2 treatmen... View More
Product Details
PA5-27240
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SOD1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.21 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, NT2D1, PC-3, SK-N-SH, Rat brain, U87-MG.
Predicted reactivity: Mouse (100%), Rat (100%), Chimpanzee (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Amyotrophic lateral sclerosis 1 (ALS1); Cu(2+)-Zn2+ superoxide dismutase; Cu/Zn SOD; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HGNC:11179; hSod1; indophenoloxidase A; Indophenoloxidase A (IPOA); SOD, soluble; superoxide dismutase; Superoxide dismutase 1; Superoxide Dismutase 1 (SOD1); Superoxide dismutase [Cu-Zn]; superoxide dismutase, cystolic
Gene Aliases: ALS; ALS1; B430204E11Rik; Cu/Zn-SOD; CuZnSOD; HEL-S-44; homodimer; hSod1; Ipo-1; Ipo1; IPOA; SOD; Sod-1; SOD1; SODC
UniProt ID: (Human) P00441, (Mouse) P08228, (Rat) P07632
Entrez Gene ID: (Human) 6647, (Mouse) 20655, (Rat) 24786
superoxide dismutase activity
copper ion binding
protein binding
zinc ion binding
protein phosphatase 2B binding
identical protein binding
protein homodimerization activity
Rac GTPase binding
chaperone binding
antioxidant activity
oxidoreductase activity
enzyme binding
metal ion binding
oxidoreductase
activation of MAPK activity
response to reactive oxygen species
response to superoxide
ovarian follicle development
positive regulation of cytokine production
placenta development
retina homeostasis
response to amphetamine
myeloid cell homeostasis
platelet degranulation
glutathione metabolic process
superoxide metabolic process
cellular iron ion homeostasis
spermatogenesis
embryo implantation
cell aging
sensory perception of sound
locomotory behavior
anterograde axonal transport
retrograde axonal transport
regulation of blood pressure
response to heat
response to organic substance
transmission of nerve impulse
removal of superoxide radicals
response to nutrient levels
peripheral nervous system myelin maintenance
positive regulation of superoxide anion generation
regulation of T cell differentiation in thymus
response to carbon monoxide
cellular response to potassium ion
regulation of multicellular organism growth
response to drug
response to hydrogen peroxide
superoxide anion generation
positive regulation of apoptotic process
positive regulation of catalytic activity
regulation of GTPase activity
negative regulation of neuron apoptotic process
response to ethanol
negative regulation of cholesterol biosynthetic process
regulation of protein kinase activity
regulation of organ growth
response to antibiotic
response to copper ion
muscle cell cellular homeostasis
thymus development
response to axon injury
hydrogen peroxide biosynthetic process
regulation of mitochondrial membrane potential
oxidation-reduction process
heart contraction
neurofilament cytoskeleton organization
relaxation of vascular smooth muscle
auditory receptor cell stereocilium organization
cellular response to cadmium ion
cellular response to ATP
reactive oxygen species metabolic process
response to antipsychotic drug
positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
response to oxidative stress
aging
negative regulation of apoptotic process
double-strand break repair
apoptotic DNA fragmentation
cellular response to oxidative stress
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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