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Invitrogen
SOD2 Polyclonal Antibody
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FIGURE: 1 / 7
SOD2 Antibody (PA1-31072) in ICC/IF
Immunofluorescence analysis of SOD2 was performed using 70% confluent log phase A549 cells treated with TNF alpha (20 ng/mL for 72h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SOD2 Polyclonal Antibody (Product # PA1-31072) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary A... View More
Product Details
PA1-31072
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Hamster,
Human,
Mouse,
Non-human primate,
Sheep,
Pig,
Rabbit,
Rat,
Xenopus
Published species
Bovine,
C. elegans,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length protein (Human).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.05M sodium phosphate, pH 7.2, with 0.1% BSA, 50% glycerol
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Rat brain tissue extract was used as a positive control.
Target Information
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD-1), mitochondrial manganese SOD (Mn-SOD/SOD-2) and extracellular Cu, Zn-SOD (EC-SOD/SOD-3). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80 kDa tetrameric enzyme SOD2, is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin-binding multimer of disulfide-linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase (CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: indophenoloxidase B; IPO B; manganese SOD; manganese superoxide dismutase; manganese-containing superoxide dismutase; mangano-superoxide dismutase; manganous superoxide dismutase; mitochondrial superoxide dismutase 2; Mn superoxide dismutase; Superoxide dimutase 2, mitochondrial; superoxide dismutase; superoxide dismutase (Mn type); superoxide dismutase 2, mitochondrial; superoxide dismutase 2, mitochondrial L homeolog; Superoxide dismutase [Mn], mitochondrial
Gene Aliases: I79_015272; IPO-B; IPOB; Mn-SOD; MNSOD; MVCD6; Sod-2; SOD2; sodMt
UniProt ID: (Bovine) P41976, (Human) P04179, (Rat) P07895, (Mouse) P09671
age-dependent response to reactive oxygen species
regulation of transcription from RNA polymerase II promoter
superoxide metabolic process
removal of superoxide radicals
oxidation-reduction process
response to reactive oxygen species
response to superoxide
release of cytochrome c from mitochondria
vasodilation by acetylcholine involved in regulation of systemic arterial blood pressure
regulation of blood pressure
negative regulation of cell proliferation
oxygen homeostasis
negative regulation of neuron apoptotic process
protein homotetramerization
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
age-dependent response to oxidative stress
response to hypoxia
liver development
detection of oxygen
glutathione metabolic process
response to oxidative stress
mitochondrion organization
heart development
aging
sensory perception of sound
locomotory behavior
intrinsic apoptotic signaling pathway in response to DNA damage
intrinsic apoptotic signaling pathway in response to oxidative stress
apoptotic mitochondrial changes
response to radiation
response to cold
post-embryonic development
response to manganese ion
response to zinc ion
response to selenium ion
response to gamma radiation
response to activity
respiratory electron transport chain
hemopoiesis
response to nutrient levels
response to lipopolysaccharide
response to L-ascorbic acid
response to silicon dioxide
response to isolation stress
response to immobilization stress
vasodilation
response to drug
response to hydrogen peroxide
superoxide anion generation
hydrogen peroxide metabolic process
negative regulation of apoptotic process
positive regulation of nitric oxide biosynthetic process
negative regulation of fat cell differentiation
response to cadmium ion
negative regulation of fibroblast proliferation
neuron development
response to axon injury
erythrophore differentiation
hydrogen peroxide biosynthetic process
regulation of catalytic activity
protein homooligomerization
response to electrical stimulus
regulation of mitochondrial membrane potential
iron ion homeostasis
response to hyperoxia
response to magnetism
cellular response to ethanol
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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