Invitrogen

SON Polyclonal Antibody

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Antibody Testing Data (2)
Published Figures (1)

SON Antibody in Immunocytochemistry (ICC/IF)

SON Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

SON Antibody (PA5-65107) in ICC/IF

Immunofluorescent staining of SON in human cell line A-431 shows localization to nuclear speckles. Samples were probed using a SON Polyclonal Antibody (Product # PA5-65107).

SON Antibody in Immunocytochemistry (ICC/IF)

SON Antibody in Western Blot (WB)

SON Antibody in Western Blot (WB)

Product Image Gallery

SON Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of SON in human cell line A-431 shows localization to nuclear speckles. Samples were probed using a SON Polyclonal Antibody (Product # PA5-65107).

SON Antibody in Western Blot (WB) — Western blot analysis of SON in human cell line RT-4 and human cell line U-251 MG. Samples were probed using a SON Polyclonal Antibody (Product # PA5-65107).

SON Antibody in Western Blot (WB) — Fig. 3 RCOR2 associates with nuclear speckles at their core region. A RCOR2 (green) and SRRM2 (red) immunolabeling in HT22 cells visualized at super-resolution confocal acquisition. Hoechst is visualized in blue color. Right panels show zoomed-in individual nuclear speckles. B Western blot analysis of RCOR2, SRRM2, SON, and SRSF7 distribution on cytosolic, nuclear and chromatin fractions of HT22 cells. GAPDH and H3 were assayed as cytosolic and chromatin markers, respectively. Western blot is representative of two independent experiments. Numbers in nuclear fractions indicate the NaCl concentration (mM) at which the soluble fraction was extracted. S: supernatant. P: pellet. C Western blot analysis of co-immunoprecipitation of RCOR2, SON and HP1alpha in nuclear soluble fractions enriched in RCOR2 from HT22 fractionation experiments. Ab: antibody used to immunoprecipitate RCOR2. IgG: immunoglobulin G used as a specificity control. D , E Western blot analysis of co-immunoprecipitation of RCOR2 and nuclear speckle components using HT22 (D) and N2A (E) total cell extracts as input. LSD1 was assayed as a positive control for RCOR2 interactors. Ab: Antibody used for immunoprecipitation of RCOR2. IgG: Immunoglobulin G used as a specificity control

Product Details

PA5-65107

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human SON. Recombinant protein control fragment (Product #RP-104639).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2662627

Product Specific Information

Immunogen sequence: TCMVSETPAMS AEPTVLASEP PVMSETAETF DSMRASGHVA SEVSTSLLVP AVTTPVLAES ILEPPAMAAP ESSAMAVLES SAVTVLESST VTVL

Highest antigen sequence identity to the following orthologs - mouse 56%, rat 54%.

Target Information

The protein encoded by this gene binds to a specific DNA sequence upstream of the upstream regulatory sequence of the core promoter and second enhancer of human hepatitis B virus (HBV). Through this binding, it represses HBV core promoter activity, transcription of HBV genes, and production of HBV virions. The protein shows sequence similarities with other DNA-binding structural proteins such as gallin, oncoproteins of the MYC family, and the oncoprotein MOS. It may also be involved in protecting cells from apoptosis and in pre-mRNA splicing. Several transcript variants encoding different isoforms have been described for this gene, but the full-length nature of only two of them has been determined. [provided by RefSeq].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Revealing RCOR2 as a regulatory component of nuclear speckles.

Epigenetics & chromatin

Rivera C,Verbel-Vergara D,Arancibia D,Lappala A,González M,Guzmán F,Merello G,Lee JT,Andrés ME

Published figure using SON polyclonal antibody (Product # PA5-65107) in Western Blot

Wed Nov 24 00:00:00 EST 2021

Revealing RCOR2 as a regulatory component of nuclear speckles.

Epigenetics & chromatin

Rivera C,Verbel-Vergara D,Arancibia D,Lappala A,González M,Guzmán F,Merello G,Lee JT,Andrés ME

Published figure using SON polyclonal antibody (Product # PA5-65107) in Western Blot

Wed Nov 24 00:00:00 EST 2021

Revealing RCOR2 as a regulatory component of nuclear speckles.

Epigenetics & chromatin

Rivera C,Verbel-Vergara D,Arancibia D,Lappala A,González M,Guzmán F,Merello G,Lee JT,Andrés ME

Published figure using SON polyclonal antibody (Product # PA5-65107) in Western Blot

Wed Nov 24 00:00:00 EST 2021

Revealing RCOR2 as a regulatory component of nuclear speckles.

Epigenetics & chromatin

Rivera C,Verbel-Vergara D,Arancibia D,Lappala A,González M,Guzmán F,Merello G,Lee JT,Andrés ME

Published figure using SON polyclonal antibody (Product # PA5-65107) in Western Blot

Wed Nov 24 00:00:00 EST 2021

Revealing RCOR2 as a regulatory component of nuclear speckles.

Epigenetics & chromatin

Rivera C,Verbel-Vergara D,Arancibia D,Lappala A,González M,Guzmán F,Merello G,Lee JT,Andrés ME

Published figure using SON polyclonal antibody (Product # PA5-65107) in Western Blot

Wed Nov 24 00:00:00 EST 2021

Bioinformatics

Protein Aliases: BASS1; Bax antagonist selected in Saccharomyces 1; Negative regulatory element-binding protein; NRE-binding protein; Protein DBP-5; Protein SON; SON3

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Gene Aliases: BASS1; C21orf50; DBP-5; DBP5; HSPC310; HSPC312; KIAA1019; NREBP; SON; SON3

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UniProt ID: (Human) P18583

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Entrez Gene ID: (Human) 6651

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nucleic acid binding DNA binding RNA binding protein binding poly(A) RNA binding RS domain binding RNA metabolism protein RNA processing factor RNA splicing factor nucleic acid metabolism protein

Process(es)

microtubule cytoskeleton organization mitotic cytokinesis mRNA processing RNA splicing negative regulation of apoptotic process regulation of mRNA splicing, via spliceosome regulation of cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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