Invitrogen

SOX11 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (17) SOX11 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

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SOX11 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of SOX11 in immersion fixed HeLa human cervical epithelial carcinoma cell line. Samples were incubated in SOX11 polyclonal antibody (Product # PA5-47557) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm.

SOX11 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of SOX11 in immersion fixed paraffin-embedded sections of human brain (hippocampus). Samples were incubated in SOX11 polyclonal antibody (Product # PA5-47557) using a dilution of 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons.

SOX11 Antibody in Western Blot (WB) — Western blot was performed using SOX11 Polyclonal Antibody (Product # PA5-47557) and a 55 kDa band corresponding to SOX11 was observed across cell lines tested except BeWo and HL-60 which are reported to be negative. Whole cell extracts (30 µg lysate) of SHSY-5Y (Lane 1), IMR-32 (Lane 2), BeWo (Lane 3)HL-60 (Lane 4) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Rabbit anti-Sheep IgG (H+L) Secondary Antibody, HRP (Product # 61-8620, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

SOX11 Antibody in ChIP Assay (ChIP) — Chromatin Immunoprecipitation (ChIP) assay of endogenous SOX11 protein using Anti-SOX11 Antibody: ChIP was performed using Anti-SOX11 Polyclonal Antibody (Product # PA5-47557) 5 µg, on sheared chromatin from SH-SY5Y cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to transcriptional start site of PRKACA, DBN1 and SAT2 satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

SOX11 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. SH-SY5Y cells were transfected with SOX11 siRNA and reduction of signal was observed in Western Blot using SOX11 Polyclonal Antibody (Product # PA5-47557). {KD}

Product Details

PA5-47557

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	3-15 µg/mL	-
Immunocytochemistry (ICC/IF)	5-15 µg/mL	-
ChIP assay (ChIP)	2.5 µg/10^6 cells	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Sheep / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived recombinant human SOX11 Val2-Pro173 View immunogen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 5% trehalose
Contains	No Preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2608887

Product Specific Information

In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) SOX1, rhSOX2, rhSOX3, rhSOX5, rhSOX6, rhSOX7, rhSOX9, rhSOX10, rhSOX12, rhSOX14, rhSOX15, rhSOX17, and rhSOX21 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

This intronless gene encodes a member of the SOX family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The protein may function in the developing nervous system and play a role in tumorigenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: SRY; SRY (sex-determining region Y)-box 11; SRY-related HMG-box gene 11; Transcription factor SOX-11

Gene Aliases: MRD27; SOX11

UniProt ID: (Human) P35716

Entrez Gene ID: (Human) 6664

Molecular Function: HMG box transcription factor

Function(s)

Process(es)

transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription coactivator activity enhancer sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding translation factor activity, RNA binding

SOX11 Polyclonal Antibody

Product Details

PA5-47557

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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