Invitrogen

SOX15 Polyclonal Antibody

View all (7) SOX15 antibodies

Datasheet

Protocols

Questions & Answers

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (2)

FIGURE: 1 / 2

SOX15 Antibody (720155) in WB

Western blot analysis was performed on whole cell extracts (50 µg lysate) of Panc-1 (Lane1), Hela (Lane 2), L6 (Lane 3), Mouse Testis (Lane 4) and Rat testis (Lane 5). The blots were probed with Anti-SOX15 Rabbit Polyclonal Antibody (Product # 720155, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 25 and 19 kDa band corresponding to isoforms of SOX15 was observed according to the cell lines and tissues tested. Kno... View More

Product Image Gallery

SOX15 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence was performed on Methanol fixed NTERA-2 cells for detection of SOX-15 using Anti-SOX-15 Rabbit Polyclonal Antibody (Product # 720155, 2 µg/mL), alpha-Tubulin Monoclonal Antibody (Product # 32-2500, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000), Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor® 594conjugate (Product # A-11032, 1:400) respectively. Panel a) shows representative cells that were stained for detection and localization of A protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938,). Panel c) represents cytoskeletal alpha-tubulin staining (red). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of SOX-15. Panel e) represents control cells with no primary Antibody to assess background.

Product Details

720155

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

Immunocytochemistry (ICC/IF)

1-2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptides corresponding to Human SOX15 (aa 121-133, 195-210, 33-48)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 30% glycerol, 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2610200

Product Specific Information

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

This antibody is predicted to react with Monkey, Pig and Goat.

Target Information

SOX15 is a member of the SOX (SRY-related HMG-box) family of transcription factors and is involved in the regulation of embryonic development and in the determination of cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Cite this product

Bioinformatics

Protein Aliases: Protein SOX-12; Protein SOX-15; Protein SOX-20; SRY (sex determining region Y)-box 15; SRY (sex determining region Y)-box 20; SRY box 15; SRY-box containing gene 15

Gene Aliases: Sox-15; SOX12; SOX15; SOX20; SOX26; SOX27

UniProt ID: (Human) O60248, (Mouse) P43267

Entrez Gene ID: (Human) 6665, (Mouse) 20670, (Rat) 363632

Molecular Function: HMG box transcription factor