Invitrogen

SOX2 Polyclonal Antibody

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SOX2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of uterus, endometrial glands using Product # PA1-16968.

SOX2 Antibody in Western Blot (WB) — Western Blot detection of SOX2 in mouse brain lysate using Product # PA1-16968 (0.5 µg/mL).

SOX2 Antibody in Flow Cytometry (Flow) — FACS staining of NTERA-2 cells using Product # PA1-16968 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary antibody.

SOX2 Antibody in Immunocytochemistry (ICC) — ICC staining of NTera2 cells using Product # PA1-16968 for SOX2 (Green) at a 1:50 dilution and an anti-alpha-Tubulin (Red) at a 1:1000 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.

SOX2 Antibody — Fig. 1 Loss of Oct4 and maintenance of Sox2 expression during retinoic acid-induced differentiation. Oct4 expression begins to decline after 24-hour exposure to retinoic acid (1 muM), with the main transition to an Oct4- state occurring between 24 and 48 h of RA treatment. After 72 h, ~ 70% of the population retain an Oct4-Sox2 + phenotype ( a , c ). A schematic depicting the change in gene expression in ESC populations towards the predominant progenitor state during two differentiation protocols: LIF-withdrawal in serum-containing media and retinoic acid addition ( b ). Quantification of flow cytometry data in a demonstrates the maintenance of the Oct4-Sox2 + state between 48 and 72 h of RA treatment ( c ), n = 9 (three biological replicates, three technical replicates for each biological replicate). Scale bar: 50 um

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1:100

Immunocytochemistry (ICC)

1:50-1:250

Immunofluorescence (IF)

1:50-1:250

Immunohistochemistry (Paraffin) (IHC (P))

1:125-1:250

Western Blot (WB)

0.5 µg/mL

Published Applications

Flow Cytometry (Flow)

See 1 publication below

Immunofluorescence (IF)

See 3 publications below

Western Blot (WB)

See 2 publications below

Immunohistochemistry (IHC)

See 1 publication below

Immunocytochemistry (ICC)

See 2 publications below

Miscellaneous PubMed (MISC)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse, Sheep

Published species

Fish, Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide made to the N-terminal region of human SOX2 protein (within residues 1-100). [Swiss-Prot# P48431]

Conjugate

Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2195781

Product Specific Information

The target sequence has 92% sequence homology with chicken, 86% sequence homology with xenopus, and 71% sequence homology with zebrafish.

Suggested positive control: antigen standard for SOX2 (transient overexpression lysate), mouse brain lysate, mouse brain protein, uterual endometrial glands.

Target Information

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ANOP3; MCOPS3; sex determining region Y box 2; sex determining region Y-box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2

Gene Aliases: ANOP3; lcc; MCOPS3; Sox-2; SOX2; ysb

UniProt ID: (Human) P48431, (Sheep) P54231, (Mouse) P48432

Entrez Gene ID: (Human) 6657, (Sheep) 101110563, (Mouse) 20674

Molecular Function: HMG box transcription factor nucleic acid binding transcription factor