Invitrogen

SOX2 Polyclonal Antibody

9 Published Figures

View all (52) SOX2 antibodies

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Questions & Answers

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SOX2 Antibody in Immunocytochemistry (ICC/IF) — ICC staining of NTera2 cells using Product # PA1-16968 for SOX2 (Green) at a 1:50 dilution and an anti-alpha-Tubulin (Red) at a 1:1000 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.

SOX2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of uterus, endometrial glands using Product # PA1-16968.

SOX2 Antibody in Western Blot (WB) — Western Blot detection of SOX2 in mouse brain lysate using Product # PA1-16968 (0.5 µg/mL).

SOX2 Antibody in Flow Cytometry (Flow) — FACS staining of NTERA-2 cells using Product # PA1-16968 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary antibody.

SOX2 Antibody — Fig. 1 Loss of Oct4 and maintenance of Sox2 expression during retinoic acid–induced differentiation. Oct4 expression begins to decline after 24-hour exposure to retinoic acid (1 μM), with the main transition to an Oct4- state occurring between 24 and 48 h of RA treatment. After 72 h, ~ 70% of the population retain an Oct4-Sox2 + phenotype (a, c). A schematic depicting the change in gene expression in ESC populations towards the predominant progenitor state during two differentiation protocols: LIF-withdrawal in serum-containing media and retinoic acid addition (b). Quantification of flow cytometry data in a demonstrates the maintenance of the Oct4-Sox2 + state between 48 and 72 h of RA treatment (c), n = 9 (three biological replicates, three technical replicates for each biological replicate). Scale bar: 50 µm {TM}

SOX2 Antibody in Immunocytochemistry (ICC/IF) — Figure 10 Expression and localization of hsa-miR-590-3p and SOX2 in SNU449 cells. (a) hsa-miR-590-3p signal in SNU449 cells under 20x magnification using ISH (red circle). (b) SOX2 fluorescent signal in SNU449 cells under 20x magnification using ICC (white circle). (c) DAPI staining of the nucleus. (d) Merged image of (b) and (c). (e) U6 signal SNU449 cells under 20x magnification using ISH as a positive control (red circles). (f) Negative control in SNU449 cells under 20x magnification using ISH using scrambled probes. Scale for (a)-(f): 20 mu m.

SOX2 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Western Blot and Immunocytochemistry

SOX2 Antibody in Immunocytochemistry (ICC/IF) — Fig 3 The SETD7 knock-down causes defects in the silencing of pluripotency genes. (A) Western blot showing the levels of OCT4 at different days during the in vitro differentiation of ES[4] transduced with a non target shRNA (shSCR) and a shRNA that targets SETD7 (shSETD7) (B) Immunolocalization of SOX2 (green) and OCT4 (red) expression at day 4 and day 15 of in vitro differentiation of ES[4] transduced with a non target shRNA (shSCR) and a shRNA that targets SETD7 (shSETD7) (C) Quantification of the percentage of embryoid bodies negative or positive for OCT4 staining at day 4 and day 7 of differentiation of cells treated with vehicle (DMSO), 1muM or 5muM PFI-2. (D) Immunolocalization of OCT4 (red) in embryoid bodies at day 4 and day 7 of in vitro differentiation of ES[4] treated with vehicle (DMSO) or 5muM PFI-2.

SOX2 Antibody in Immunocytochemistry (ICC/IF) — Published figure using SOX2 polyclonal antibody (Product # PA1-16968) in Immunocytochemistry

SOX2 Antibody in Immunohistochemistry (IHC) — Figure 7 Immunohistochemistry on tissue microarrays of GBM for YBX1 and its interacting proteins - EGFR, MAPK1, CD44, TNC, MMP13 and SOX2. IHC analysis of the proteins tested using in-house prepared tissue microarrays, confirmed overexpression of YBX1 and its interacting proteins in multiple tumor specimens. The details of tissue microarrays used and IHC procedure are described in the Methods and the staining scoring details are shown in Supplementary Table S11 .

SOX2 Antibody in Western Blot (WB) — Figure 8 SOX2 mRNA and protein expression in HepG2 and SNU449 using RT-PCR and western blotting, respectively. (a) Graphical representation of SOX2 mRNA expression in HepG2 and SNU449 using RT-PCR. (b) Graphical representation of SOX2 protein expression using western blotting. RT-PCR and western blotting band intensities were measured using ImageJ and normalized against GAPDH and beta -tubulin, respectively, and statistically analyzed using Prism GraphPad. P values were computed using one-way ANOVA (with a Bonferroni posttest). P values less than 0.05 are considered significant ( * P value < 0.05, ** P value < 0.01, and *** P value < 0.001). Negatives were carried out for RT-PCR experiments. Results are a representation of three independent experiments.

SOX2 Antibody in Western Blot (WB) — Fig 1 SETD7 is expressed at very low levels in pluripotent human cells and induced during differentiation. (A) Average rank of the top 700 most differentially expressed genes between pluripotent (iPSCs or ESCs) and fibroblasts, including those upregulated in pluripotent cells (left panel) and upregulated in fibroblasts (right panel). (B) SETD7 mRNA levels in human ESCs grown under self-renewal conditions (UndES[4]), in vitro differentiated human ESCs (DifES[4]), human fibroblasts (HFF), two lines of human keratinocytes (HEK1 and HEK2) and two lines of iPSCs generated from keratinocytes ([H]KiPS4F and KiPS4F1). Mean and standard deviation of three technical replicates is shown. Induction of SETD7 mRNA levels during ES[4] differentiation was confirmed in more than four independent differentiation experiments. (C) Western blot showing SETD7 protein levels in pluripotent and somatic cells. Loading control beta actin (ACTB) is also shown. (D) Western blot showing protein levels of SETD7, AFP, OCT4 and SOX2 in under self-renewing conditions and in vitro differentiated human ESCs. Loading control alpha tubulin (TUBA) is also shown. One representative experiment out of three is shown. (E) Genomic visualization of the levels of H3K72me3, H3K4me3, H3K4me2, H3K36me3 and RNA polymerase II (Pol II) in the human embryonic stem cell line H1 around the SETD7 gene according to ENCODE. A non-methylated CpG island is depicted in green. (F) Levels of H3K4me2 and H3K27me3 at SETD7 gene promoter r

SOX2 Antibody in Flow Cytometry (Flow) — Fig. 1 Loss of Oct4 and maintenance of Sox2 expression during retinoic acid-induced differentiation. Oct4 expression begins to decline after 24-hour exposure to retinoic acid (1 muM), with the main transition to an Oct4- state occurring between 24 and 48 h of RA treatment. After 72 h, ~ 70% of the population retain an Oct4-Sox2 + phenotype ( a , c ). A schematic depicting the change in gene expression in ESC populations towards the predominant progenitor state during two differentiation protocols: LIF-withdrawal in serum-containing media and retinoic acid addition ( b ). Quantification of flow cytometry data in a demonstrates the maintenance of the Oct4-Sox2 + state between 48 and 72 h of RA treatment ( c ), n = 9 (three biological replicates, three technical replicates for each biological replicate). Scale bar: 50 um

SOX2 Antibody — Figure 11 Graphical representation of the expression of hsa-miR-590-3p and SOX2 in SNU449 cells. The cell fluorescence of SOX2 and the signal intensity of hsa-miR-590-3p were assessed using ImageJ and compared using Prism GraphPad. Low-expressing hsa-miR-590-3p cells show increased SOX2-corrected total cell fluorescence (CTCF) compared to high-expressing has-miR-590-3p cells, which show low SOX2 CTCF. Results are a representation of three independent experiments.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:125-1:250

-

Immunocytochemistry (ICC/IF)

1:50-1:250

View 6 publications 6 publications

Flow Cytometry (Flow)

1:100

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Sheep

Published species

Fish, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide made to the N-terminal region of human SOX2 protein (within residues 1-100). [Swiss-Prot# P48431]

Conjugate

Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2195781

Product Specific Information

The target sequence has 92% sequence homology with chicken, 86% sequence homology with xenopus, and 71% sequence homology with zebrafish.

Suggested positive control: antigen standard for SOX2 (transient overexpression lysate), mouse brain lysate, mouse brain protein, uterual endometrial glands.

Target Information

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: sex determining region Y-box 2; SOX 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY-box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2

Gene Aliases: ANOP3; lcc; MCOPS3; Sox-2; SOX2; ysb

UniProt ID: (Human) P48431, (Sheep) P54231, (Mouse) P48432

Entrez Gene ID: (Human) 6657, (Sheep) 101110563, (Mouse) 20674

Molecular Function: HMG box transcription factor

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation eye development endodermal cell fate specification chromatin organization regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell cycle arrest response to wounding regulation of gene expression glial cell fate commitment pituitary gland development adenohypophysis development positive regulation of cell-cell adhesion forebrain development somatic stem cell population maintenance tissue regeneration regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade positive regulation of cell differentiation negative regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter inner ear development negative regulation of epithelial cell proliferation response to growth factor negative regulation of canonical Wnt signaling pathway neuronal stem cell population maintenance cell fate specification positive regulation of neuroblast proliferation transcription, DNA-templated regulation of transcription from RNA polymerase II promoter multicellular organism development sensory perception of sound response to organic substance stem cell population maintenance forebrain neuron differentiation cerebral cortex development negative regulation of Wnt signaling pathway male genitalia development positive regulation of epithelial cell differentiation olfactory placode formation response to retinoic acid inner ear morphogenesis tongue development cell fate commitment negative regulation of cell differentiation positive regulation of neuron differentiation negative regulation of osteoblast differentiation positive regulation of Notch signaling pathway pigment biosynthetic process lung alveolus development embryonic organ development anatomical structure formation involved in morphogenesis neuron fate commitment diencephalon morphogenesis stem cell differentiation regulation of neurogenesis detection of mechanical stimulus involved in sensory perception of sound detection of mechanical stimulus involved in equilibrioception retina morphogenesis in camera-type eye lens induction in camera-type eye epithelial tube branching involved in lung morphogenesis

Process(es)

transcription regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding miRNA binding sequence-specific DNA binding transcription regulatory region DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding chromatin binding transcription factor binding chromatin DNA binding protein heterodimerization activity